66 research outputs found
Research Notes : Inheritance of hard seeds in soybeans
During the past three years we have studied the inheritance of hard seeds in soybeans. These studies have been supported in part by INTSOY and in part by the Rockefeller Foundation. They were begun with the help of Dr. H. C. Minor and Dr. E. H. Paschal III who had evaluated potential parental material for the hard-seed characteristic and who continued to help through advice, handling plant materials in Puerto Rico, and providing certain facilities
Dissecting Quantitative Trait Loci for Agronomic Traits Responding to Iron Deficeincy in Mungbean [Vigna Radiata (L.) Wilczek]
Calcareous soil is prevalent in many areas of the world agricultural land causing substantial yield loss of crops. We previously identified two quantitative trait locus (QTL) qIDC3.1 and qIDC2.1 controlling leaf chlorosis in mungbean grown in calcareous soil in two years (2010 and 2011) using visual score and SPAD measurement in a RIL population derived from KPS2 (susceptible) and NM10-12-1 (resistant). The two QTLs together accounted for 50% of the total leaf chlorosis variation and only qIDC3.1 was confirmed, although heritability estimated for the traits was as high as 91.96%. In this study, we detected QTLs associated with days to flowering , plant height, number of pods per plants, number of seeds per pods, and seed yield per plants in the same population grown under the same environment with the aim to identify additional QTLs controlling resistance to calcareous soil in mungbean. Single marker analysis revealed 18 simple sequence repeat markers, while composite interval mapping identified 33 QTLs on six linkage groups (1A, 2, 3, 4, 5 and 9) controlling the five agronomic traits. QTL cluster on LG 3 coincided with the position of qIDC3.1, while QTL cluster on LG 2 was not far from qIDC2.1. The results confirmed the importance of qIDC3.1 and qIDC2.1 and revealed four new QTLs for the resistance to calcareous soil
Qtls Controlling Seed Weight and Days to Flowering in Mungbean [Vigna Radiata (L.) Wilczek], Their Conservation in Azuki Bean [V. Angularis (Ohwi) Ohwi & Ohashi] and Rice Bean [V. Umbellata (Thunb.) Ohwi & Ohashi]
Mungbean (Vigna radiata (L.) Wilczek) is a socio-economically important legume crop of Asia. Varieties with large seed size and early maturity are preferred in commercial production. In this study, we identified quantitative trait loci (QTL) controlling seed weight and days to flowering in mungbean. The mapping population comprises 155 F2-derived lines from a cross between Kamphaeng Saen 1 (large-seeded and early flowering) and V4718 (small-seeded and late flowering). The F2 population was analyzed with 67 simple sequence repeat markers. The F2:3 families were evaluated for 100-seed weigh and days to flowering in two years, 2008 (one season) and 2011 (two seasons). Composite interval mapping identified six QTLs for 100-seed weight and 5 QTLs for days to flowering. Three genomic regions harbored QTLs for both seed weight and days to flowering, revealing association between the two traits. Comparison of QTLs for both traits found in this study with those reported in azuki bean (Vigna angularis (Willd.) Ohwi & Ohashi) and rice bean (Vigna umbellata (Thunb.) Ohwi & Ohashi) revealed that several QTLs are conserved among the three Vigna species
Gene Mapping of a Mutant Mungbean (Vigna radiata L.) Using New Molecular Markers Suggests a Gene Encoding a YUC4-like Protein Regulates the Chasmogamous Flower Trait
Mapping QTL for bruchid resistance in rice bean (Vigna umbellata)
This research articles was published in Euphytica journal, volume 207, 2016The damage caused to stored seed by bruchids (Callosobruchus maculatus) is considered to be a major production constraint in rice bean (Vigna umbellata). Breeding for genetically determined resistance is the most environmentally benign and cost-effective means to mitigate the losses to bruchid infestation. Here, a screen of rice bean germplasm identified two sources of resistance, and determined the genetic basis of the resistance using a quantitative trait locus (QTL) mapping approach. The two resistant accessions (LRB238 and JP100304) were each crossed to a common susceptible cultivar (LRB26) to generate F2 mapping populations, one of which (LRB238 × LRB26) was genotyped with a range of Vigna sp. microsatellite assays and by sequence related amplified polymorphism (SRAP) fingerprinting. The resulting linkage map comprised ten linkage groups and covered a genetic distance of 872.1 cM with a mean inter-marker distance of 32.05 cM. The subsequent QTL analysis detected the presence of 11 QTL, distributed over all ten linkage groups, most of which were associated with the % damage caused to the seed. Two major QTL, Cmpd1.5 (flanked by the SRAP markers E2M9-270 and E12M7-311) and Cmpd1.6 (flanked by the SRAP marker E7M10-141 and the microsatellite locus CEDG259) mapped within 11.9 cM and 13.0 cM of the flanking markers, respectively, accounted for, 67.3 and 77.4 % of the variance respectively, for % damaged seeds. A bulked segregation analysis carried out in the JP100304 × LRB26 population revealed that the resistance donor harboured some resistance factors not represented in LRB238
タイにおける耐暑性アズキ近縁野生種の分布調査
To seek new gene sources for improved high temperature tolerance in azuki bean (Vigna angularis), a collaborative expedition to collect wild relatives in the genus Vigna was conducted from 15th November to 12nd December, 1999. A total of 62 samples, 14 of Vigna exilis, 1 of V. grandiflora, 3 of V. hirtella, 9 of V. minima, 3 of V. tnnervia, 31 of V. umbellata and 1 of V. unguiculata were collected. In addition to seed samples, nodule samples and herbarium specimens were also collected
Variation and Inheritance of Hardseededness in Soybeans (Glycine Max (L.) Merr.)
88 p.Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1980.A series of experiments dealing with variation as well as inheritance of hardseededness in soybeans was conducted from July 1977 to July 1979. Field and greenhouse plantings were made at Isabela Experiment Station, Puerto Rico and the Department of Agronomy, University of Illinois, respectively.Three regression models (linear, second degree polynomial, and exponential) were employed to explain the relationship between days after putting seeds in a germinator and hard seed percentage in two soybean varieties, Barchet and PI 326578. The exponential regression model was the best in explaining the relationship using field data whereas the second degree polynomial model was the best using greenhouse data. All models fitted the data better when first day observations were excluded.Two determinate varieties (Barchet and PI 326578) and two indeterminate varieties (PI 307865 and PI 323566) were grown in the field to study relationships between positions of seeds on a plant and hard seed percentages. Relationships were detected between hard seed percentages and nodal positions on the main stems of Barchet and PI 307865 and on the primary branches of PI 323566. No relationship was found between hard seed percentages and seed positions in a pod.When field grown seeds from Barchet and PI 326578 were planted in the greenhouse, the resulting plants grown from normal seeds yielded hard seed percentages similar to those from plants grown from hard seeds. No correlation was found in proportion of hard seeds from a plant grown in a different environment. Percentages of hard seeds were much higher on greenhouse grown than on field grown plants. Genotype by environment interaction of this trait was also observed.Inheritance studies using crosses between low and high hard seed percentage varieties (Barchet x Hardee, Barchet x SJ 2, Hardee x PI 326578, and SJ 2 x PI 326578) indicated that two to four loci of major genes controlled the observed variation in hard seed percentages. Genes for normal seeds were dominant. Modifiers seemed to involve in variation of this trait in the cross between low and medium hard seed percentage varieties (Barchet x PI 323566), as well as in the cross between high hard seed percentage varieties (Barchet x 326578), however, the former cross displayed a heterotic effect in the progenies whereas the latter showed only minor (modifier) genes with additive effects.A linkage probably exists between one major gene locus for hardseededness and the i locus which is involved in seed and coat color.U of I OnlyRestricted to the U of I community idenfinitely during batch ingest of legacy ETD
Research Notes : Inheritance of hard seeds in soybeans
During the past three years we have studied the inheritance of hard seeds in soybeans. These studies have been supported in part by INTSOY and in part by the Rockefeller Foundation. They were begun with the help of Dr. H. C. Minor and Dr. E. H. Paschal III who had evaluated potential parental material for the hard-seed characteristic and who continued to help through advice, handling plant materials in Puerto Rico, and providing certain facilities.</p
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