Geographic distribution of European, African and Asian samples.

<p>See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0134840#pone.0134840.s012" target="_blank">S1 Table</a> for abbreviation codes. Symbols: “▪” samples genotyped in this study, “+” data from the 1000 Genomes Project.</p

Plot of F_ST values of the markers under potential positive selection found in the F_ST vs heterozygosity analysis.

<p>On the left markers found only in the context of the three continents, on the right markers found only in Europe and North Africa and in the middle (grey) the SNPs identified in both analyses.</p

Significant positive selection results in a minimum of two different tests.

<p>Observed (Obs) F_ST with selection significance (highlighted P value<0.01) for the three continents (Africa, Asia and Europe) and for Europe and North Africa. Global cross-continental F_ST scores in the CEU, CHB, and YRI samples from the 1000 Genomes Selection Browser. Significant high and low FST values (extreme top 5% values of the distribution of F_ST values across the genome) are highlighted in bold. P values were calculated on the basis of higher rank scores in the genomic distribution. SPA scores for continental European, and North African allele gradients (highlighted in bold SPA scores above 99th percentile).</p

Contour map of the MAF of the CAD risk SNP rs1333042 potentially positive selected in the 9p21 region.

<p>Contour map of the MAF of the CAD risk SNP rs1333042 potentially positive selected in the 9p21 region.</p

LD blocks for the region 10q11 in 26 populations from Europe, Africa and Asia.

<p>Markers previously associated with CAD only in Europe are highlighted in blue and in North Africa in red.</p

Significant balancing selection results in a minimum of two different tests.

<p>Observed (Obs) F_ST with selection significance (highlighted P value<0.01) for the three continents (Africa, Asia and Europe), and for Europe and North Africa. Global cross-continental F_ST scores and Tajima’s D test results in the CEU, CHB, and YRI samples from the 1000 Genomes Selection Browser. Significant high and low F_ST values (extreme top 5% values of the distribution of F_ST values across the genome), and signals of potential balancing selection (P value≤0.05) are highlighted in bold. Both F_ST and Tajima D P values were calculated on the basis of higher rank scores in the genomic distribution.</p

PC plot of the 22 population from Europe (circle), Middle East (circle) and North Africa (diamond) based on the variation of 190 independent SNPs located in the CAD risk regions 1p13, 1q41, 9p21 and 10q11.

<p>PC plot of the 22 population from Europe (circle), Middle East (circle) and North Africa (diamond) based on the variation of 190 independent SNPs located in the CAD risk regions 1p13, 1q41, 9p21 and 10q11.</p

LD difference p-values for the 4 genomic regions analysed.

<p>Pairwise comparisons between North Europe (NEUR), South Europe (SEUR), North Africa (NAFR), Sub-Saharan Africa (SAFR), and Asia.</p

LD blocks for the region 9p21 in 26 populations from Europe, Africa and Asia.

<p>Markers previously associated with CAD only in Europe are highlighted in blue, in Caucasians together with African Americans and Chinese in black and in North Africa in red.</p

Meta-analyses association parameters of risk variants with P value<0.1 in both analysis (from MACH and IMPUTE2 datasets) after being pruned by LD lower than 0.2.

<p>SE: Standard Error.</p