2 research outputs found
PHYTOCHEMICAL ANALYSIS AND ESTIMATION OF ANTI OXIDANT POTENTIAL OF PHYTOSOMES FORMULATIONS OF MORINDA LUCIDA BENTH
Introduction: Â Lucid morinda (L) A medium-sized evergreen tree of the Morinda genus, the Benth (M. lucida, Rubiaceae) is a tropical West African rainforest plant that is also known as the Brimstone tree. In several West African nations, it is utilized in traditional medicine to cure a variety of human ailments. Phytosomes are freshly developed herbal formulations that have superior bioavailability and effects than traditional phyto molecules or botanical extracts because they are more readily absorbed. The goals of the current study were to evaluate ethanolic leaf extract of M. lucida's phytosomes, in vitro antioxidant properties, qualitative and quantitative phytochemical analyses, and phytosome formation.
Methods: The ethanolic plant extract and cholesterol-containing phospholipids were used to make the phytosome. The entrapment efficiency, particle size and size distribution, optical microscopic research, stability tests, and in vitro dissolution investigations were used to characterize phytosomes. Alkaloids, glycosides, sterols, phenols, terpenoids, tannins, and saponins were found by phytochemical examination.
Results: The ethanolic leaf extract of M. lucida contained 0.721 mg of total phenols and 0.464 mg of total alkaloids per 100 mg. Using industry-standard procedures, the ethanolic leaf extract's in vitro antioxidant activity was evaluated against the DPPH assay technique. Combining phospholipids and M. lucida can have a synergistic impact, which can be measured using the DPPH model for free radical scavenging activity. The optimised batch F10's particle size and entrapment efficiency were found to be 223.30±0.41 nm and 76.46±0.61 nm, respectively.
Conclusion: It can be employed as a targeted medicine delivery system in the future for applications such as liver, brain, heart, etc. protection. The use of novel approaches rather than traditional methods for delivering herbal drugs increases the bioavailability of polar extracts and boosts patient compliance.
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Peer Review History:
Received: 28 October 2022; Revised: 27 November; Accepted: 3 January 2023, Available online: 15 January 2023
Academic Editor: Dr. Gehan Fawzy Abdel Raoof Kandeel, Pharmacognosy Department, National Research Centre, Dokki, 12622, Giza, Egypt, [email protected]Â
Received file:Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Reviewer's Comments:
Average Peer review marks at initial stage: 5.5/10
Average Peer review marks at publication stage: 7.0/10
Reviewers:
Dr. George Zhu, Tehran University of Medical Sciences, Tehran, Iran, [email protected]
Dr. Hasniza Zaman Huri, University of Malaya Medical Centre, Kuala Lumpur, [email protected]
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IN VITRO ASSESSMENT OF ANTIMICROBIAL AND ANTIOXIDANT ACTIVITIES OF EXTRACTS OF YEMENI FAGONIA SCHWEINFURTHII HADIDI
CYTOTOXIC EFFECT, ANTIOXIDANT POTENTIAL, AND PHYTOCHEMICAL STUDY OF THE ETHYL ACETATE EXTRACT OF PLEIOGYNIUM TIMORENSE SEED
PHYTOCHEMICAL SCREENING AND ANTIDIABETIC ACTIVITY OF METHANOLIC EXTRACT OF CAYLUSEA ABYSSINICA LEAVES
Introduction: Â Controlling type 2 diabetes with a treatment having no side effects remains a challenge for researchers even if the side effects are reduced and there may be a chance for reduced adverse reactions or severe side effects due to drug interaction. These interactions could result from concurrent use of dietary supplements or pharmacological therapy in addition to the medications. The current reading's objective was to assess the qualitative and quantitative phytochemical analyses, and antidiabetic potentials of methanol leaf extract of Caylusea abyssinica (C. abyssinica) in diabetic rats.
Methods: The qualitative and quantitative phytochemical analyses of total alkaloids phenol, and flavonoids were determined using the well-known test procedure outlined in the literature. The obtained C. abyssinica methanol leaf extract was used to examine in vitro anti-diabetic activity (α-Amylase inhibition), oral acute toxicity, and in vivo anti-diabetic activity against streptozotocin-induced diabetes rat model. Alkaloids, carbohydrates, reducing sugars, saponins, phenolic compounds, glycosides, tannins, and flavonoids were all established in the samples after phytochemical examination.
Results: The amount of phenolics in the methanol leaf extract was (295.50 mg/g), with flavonoids coming in second (136.66 mg/g) and alkaloids coming in third (11.23 mg/g). The extract's ability to inhibit α-amylase was investigated. The study's findings show that the chosen plants had significant in vitro anti-diabetic effect. Up to 2000 mg/kg given over 14 days, the methanol extract's acute toxicity trials did not reveal any harmful effects. Rats were given streptozotocin (60 mg/kg; i.p.) to induce diabetes, and glibenclamide (500 mcg/kg body weight) was utilized as the usual medication. Body weight, HDL, total protein, SGOT, SGPT, cholesterol, blood sugar levels, and triglycerides were all assessed in this study. Comparing diabetic rats to normal (control) rats, blood sugar and total protein, SGOT, SGPT, cholesterol levels, and triglyceride concentrations were all considerably (p<  0.001) reduced after oral administration of methanol extract of C. abyssinica at doses of 100 and 200 mg/kg and increased the level of HDL and body weight.
Conclusion: As a result of the aforementioned findings, it can be said that C. abyssinica methanol leaf extract significantly reduces blood sugar levels in streptozotocin-induced diabetic rats.
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Peer Review History:
Received: 5 November 2022; Revised: 11 December; Accepted: 8 January 2023, Available online: 15 January 2023
Academic Editor: Dr. Tamer Elhabibi, Suez Canal University, Egypt, [email protected]
Received file:Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Reviewer's Comments:
Average Peer review marks at initial stage: 5.0/10
Average Peer review marks at publication stage: 7.0/10
Reviewers:
Dr. Sangeetha Arullappan, Universiti Tunku Abdul Rahman, Malaysia, [email protected]
Prof. Hüsniye Kayalar, Ege University, Turkey, [email protected]
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ANTIDIABETIC AND ANTIHYPERLIPIDEMIC ACTIVITY OF DRACAENA CINNABARI BALF. RESIN ETHANOLIC EXTRACT OF SOQATRA ISLAND IN EXPERIMENTAL ANIMALS
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