Dietary Lutein Attenuates Bleomycin-Induced Pulmonary Fibrosis in Mice

Idiopathic Pulmonary Fibrosis is a chronic lung disease characterized by lung inflammation and progression to fibrosis. Anti-fibrotic efficacy of lutein was studied in mouse model of bleomycin induced pulmonary fibrosis. The mice were orally administrated with 100 or 200 mg/kg of lutein three days prior to intra-tracheal instillation of bleomycin on day 0 and continued up to day 21. Total cell counts and protein levels in branchoalveolar lavage fluid and superoxide dismutase, and hydroxyproline levels were evaluated. Treatment with lutein at 100 and 200 mg/kg prevented bleomycin induced mortality and body weight loss. Lutein administration attenuated bleomycin induced increase of total and differential cell count and myeloperoxidase, inflammatory cell infiltration in lung tissue. Similarly lutein administration restored superoxide dismutase activity lowered by bleomycin instillation. The combined results revealed the protective effect of lutein by suppressing the inflammation, oxidative stress and enhancing the antioxidant potential which may helps in pulmonary fibrosis

ORAL ADMINISTRATION OF BOSENTAN ATTENUATES THE BLEOMYCIN INDUCED PULMONARY FIBROSIS IN MICE

Idiopathic Pulmonary Fibrosis (IPF) is characterized by alveolitis with epithelial, endothelial damage leading to fibrosis. As signaling of endothelin-1 was involved in IPF, the effect of bosentan a non-specific endothelin receptor antagonist was determined in a mouse model of bleomycin induced pulmonary fibrosis. In the present study, mice were instilled with intra-tracheal instillation of bleomycin (0.05U) and were administered with bosentan at 100 mg/kg body weight. The treatment with bosentan significantly (p ≤ 0.05) prevented bleomycin induced mortality and loss of body weight. On day 7, bosentan significantly (p ≤ 0.05) attenuated bleomycin induced increase of total and differential inflammatory cell counts, total proteins, edema, MPO activity and inflammatory cell infiltration in lung tissue. The activities of superoxide dismutase and catalase were restored by bosentan treatment which lowered in bleomycin administered mice. Bosentan treatment significantly attenuated bleomycin induced increase in fibrosis score, collagen deposition and hydroxyproline levels. On day 21, treatment with bosentan significantly attenuated α-smooth muscle actin and collagen-I gene expression levels and matrix metalloproteinases 2 and 9 activities. The results revealed that bosentan exerted enhanced protection against bleomycin induced inflammation and fibrosis