Examining Potential Mechanisms for Increasing Emotional Willingness

Research has demonstrated that women who have experienced a rape are at an increased risk for developing subsequent psychological and behavioral consequences (e.g., mood disturbances, anxiety symptoms, substance abuse). More recently, it has been suggested that an unwillingness to experience negative emotions may contribute to these adverse consequences. One proposed strategy for increasing emotional willingness, and thereby decreasing these psychological and behavioral consequences, is to increase acceptance of one's emotional experiences. This investigation examined whether an experimental manipulation designed to increase emotional acceptance resulted in greater emotional willingness among rape survivors. Participants consisted of 38 women who experienced a rape since the age of 18. Participants were assigned to one of three conditions (acceptance, distraction, time control) and instructed to practice the skills provided during the session and record their experiences for a week. At the end of this week, participants' emotional willingness and ability to engage in functional behaviors when distressed were assessed by a trauma-relevant, distressing behavioral task. Participants also completed a self-report measure to assess for emotional willingness. Although group conditions did not differ in emotional willingness as assessed by the behavioral task, the acceptance and the time control conditions reported significantly greater increase in emotional willingness as compared to the distraction condition. Furthermore, findings suggested that differences in emotional willingness may be partially mediated by self-report non-reactivity to emotional experiences for the acceptance condition. Time control condition demonstrated decreased ability to engage in a goal-directed behavior when distressed whereas the acceptance and distraction condition did not. Finally, results suggest that distraction skills may be perceived as less tolerable based on greater non-completer rates and lower rated agreement with provided skills as compared to acceptance skills. Implications and future directions are further discussed

IMPAIRMENT IN FACIAL AFFECT RECOGNITION: DEFICIT OR ANXIETY?

Although individuals with social phobia are generally considered to exhibit social skills deficits, the existence of a potential deficit in the area of facial affect recognition remains largely unexplored. The current study investigated if individuals with high social anxiety are less able to accurately determine facial affect as compared with individuals with low social anxiety. Furthermore, this study examined whether or not this impairment is an actual deficit or results from an increased level of anxiety. Fifty-nine subjects completed an affect-labeling task at a baseline level of anxiety and again following a 5 minute speech designed to elicit anxiety. Results indicated that socially anxious and non-socially anxious individuals did not differ in accuracy of facial affect identification either at a baseline level or after engaging in a moderately stressful public performance. Based on these results, facial affect recognition does not appear to represent a skills deficit in socially anxious individuals

The Association Between Heroin Use and Anxiety Sensitivity Among Inner-City Individuals in Residential Drug Use Treatment

The current study represents an initial investigation of the association between heroin use and anxiety sensitivity (AS). Within a sample of 172 inner-city treatment seeking drug users, AS was compared across past year (1) heroin users with no crack/cocaine use (n=12); (2) crack/cocaine users with no heroin use (n=66); (3) users of both heroin and crack/cocaine (n=45); and (4) individuals with no use of heroin or crack/cocaine (n=49). Consistent with expectations, primary heroin users evidenced higher levels of AS than all other groups, with these differences also evidenced for the physical and social subscales. Differences in AS total score and physical subscale score persisted after controlling for demographic variables, depressive symptoms, and primary use of drugs other than heroin and crack/cocaine including alcohol, nicotine, marijuana, and hallucinogens. Findings suggest a unique relationship between AS and heroin, and set the stage for future work explicating the direction of the observed association

Temperamental and Environmental Risk Factors for Borderline Personality Disorder Among Inner-City Substance Users in Residential Treatment

Borderline Personality Disorder (BPD) is widely considered the result of biological vulnerability and environmental adversity. Despite growing evidence for the role of several temperamental and environmental risk factors in the development of BPD, the unique contribution of each to the development of this disorder remains unclear. Furthermore, the extent to which these factors are associated with BPD among underserved and diverse populations is unknown. The current study examined the temperamental and environmental factors uniquely associated with BPD among a sample of 93 inner-city individuals receiving residential substance use treatment. Results indicate that BPD was associated with higher impulsivity and emotional instability/vulnerability, lower well-being, and several interpersonal manifestations of positive and negative temperament (i.e., greater alienation and lower achievement and social closeness). BPD was also associated with several forms of childhood maltreatment, including emotional and physical abuse and neglect. However, only emotional instability or vulnerability, impulsivity, and emotional abuse emerged as unique predictors of BPD status

Anxiety Sensitivity: A Unique Predictor of Dropout Among Inner-City Heroin and Crack/Cocaine Users in Residential Substance Use Treatment

The present study examined the extent to which anxiety sensitivity (AS) at treatment entry was related to prospective treatment dropout among 182 crack/cocaine and/or heroin dependent patients in a substance use residential treatment facility in Northeast Washington DC. Results indicated that AS incrementally and prospectively predicted treatment dropout after controlling for the variance accounted for by demographics and other drug use variables, legal obligation to treatment (i.e., court ordered vs. self-referred), alcohol use frequency, and depressive symptoms. Findings are discussed in relation to the role of AS in treatment dropout and substance use problems more generally

Carbomer-based adjuvant elicits CD8 T-cell immunity by inducing a distinct metabolic state in cross-presenting dendritic cells.

There is a critical need for adjuvants that can safely elicit potent and durable T cell-based immunity to intracellular pathogens. Here, we report that parenteral vaccination with a carbomer-based adjuvant, Adjuplex (ADJ), stimulated robust CD8 T-cell responses to subunit antigens and afforded effective immunity against respiratory challenge with a virus and a systemic intracellular bacterial infection. Studies to understand the metabolic and molecular basis for ADJ's effect on antigen cross-presentation by dendritic cells (DCs) revealed several unique and distinctive mechanisms. ADJ-stimulated DCs produced IL-1β and IL-18, suggestive of inflammasome activation, but in vivo activation of CD8 T cells was unaffected in caspase 1-deficient mice. Cross-presentation induced by TLR agonists requires a critical switch to anabolic metabolism, but ADJ enhanced cross presentation without this metabolic switch in DCs. Instead, ADJ induced in DCs, an unique metabolic state, typified by dampened oxidative phosphorylation and basal levels of glycolysis. In the absence of increased glycolytic flux, ADJ modulated multiple steps in the cytosolic pathway of cross-presentation by enabling accumulation of degraded antigen, reducing endosomal acidity and promoting antigen localization to early endosomes. Further, by increasing ROS production and lipid peroxidation, ADJ promoted antigen escape from endosomes to the cytosol for degradation by proteasomes into peptides for MHC I loading by TAP-dependent pathways. Furthermore, we found that induction of lipid bodies (LBs) and alterations in LB composition mediated by ADJ were also critical for DC cross-presentation. Collectively, our model challenges the prevailing metabolic paradigm by suggesting that DCs can perform effective DC cross-presentation, independent of glycolysis to induce robust T cell-dependent protective immunity to intracellular pathogens. These findings have strong implications in the rational development of safe and effective immune adjuvants to potentiate robust T-cell based immunity