Autophagic marker in plantaris muscle.

<p>Plantaris LC3-I (<b>A</b>) and LC3-II (<b>B</b>) protein levels, LC3-II/LC3-I ratio (<b>C</b>) and representative imunnoblots (<b>D</b>) in Sham and MI groups. Correlation between plantaris LC3-II protein expression and distance run in a graded treadmill exercise test (<b>E</b>, Sham n = 9, MI n = 10). Data presented as mean ± SEM. AU, arbitrary unit. The number of animals in each analysis is shown within the bars.</p

qRT-PCR Primer Sequences.

<p>qRT-PCR Primer Sequences.</p

Mitophagy and mitochondrial fission in soleus muscle.

<p>Soleus Bnip3 (<b>A</b>), DRP1 (<b>B</b>) and Fis1 (<b>C</b>) protein levels, and representative immunoblots (<b>D</b>) in Sham and MI groups. Correlation between soleus <i>BNIP3</i> mRNA levels and distance run in a graded treadmill exercise test (<b>E</b>, Sham n = 8, MI n = 10). Data presented as mean ± SEM. The number of animals in each analysis is shown within the bars.</p

Skeletal muscle autophagy-related genes expression.

<p>Soleus (<b>A</b>) and plantaris (<b>B</b>) <i>BECN1</i>, <i>ATG7</i>, <i>MAP1LC3B</i>, <i>GABARAPL1</i>, <i>ATG12</i>, <i>BNIP3</i>, <i>LAMP2</i> and <i>CTSL1</i> mRNA levels of in Sham and MI groups. Data presented as mean ± SEM. *indicates p≤0.05 vs. Sham. In soleus muscle were analyzed 11 animals in Sham group and 10 animals in MI group. In plantaris muscle were analyzed 11 animals in each group.</p

Skeletal muscle oxidative stress.

<p>Soleus (<b>A</b>) and plantaris (<b>B</b>) lipid hydroperoxide levels in Sham and MI groups. Correlation between lipid hydroperoxide levels and Bnip3 protein expression in soleus (<b>C</b>, Sham n = 8, MI n = 8) and plantaris (<b>D</b>, Sham n = 10, MI n = 11) muscles in Sham and MI groups. Data presented as mean ± SEM. *indicates p≤0.05 vs. Sham. AU, arbitrary unit. The number of animals in each analysis is shown within the bars.</p

Exercise tolerance and skeletal muscle trophicity.

<p>Exercise tolerance measured by distance run (<b>A</b>), soleus weight corrected to body weight (BW) (<b>B</b>), plantaris weight corrected to BW (<b>C</b>), soleus (<b>D</b>) and plantaris (<b>E</b>) type I and type II fiber cross-sectional area (CSA) in Sham and MI groups. Data are presented as mean ± SEM. *indicates p≤0.05 vs. Sham. The number of animals in each analysis is shown within the bar.</p

Skeletal muscle Cathepsin L.

<p>Soleus (<b>A</b>) and plantaris (<b>B</b>) Cathepsin L activity in Sham and MI groups. Correlations between <i>CTSL1</i> mRNA levels and skeletal muscle fiber cross-sectional area (CSA) in soleus (<b>C</b>, Sham n = 7, MI n = 5) and plantaris (<b>D</b>, Sham n = 6, MI n = 6) muscles. Data presented as mean ± SEM. *indicates p≤0.05 vs. Sham. The number of animals in each analysis is shown within the bars.</p

Mitophagy and mitochondrial fission in plantaris muscle.

<p>Plantaris Bnip3 (<b>A</b>), DRP1 (<b>B</b>) and Fis1 (<b>C</b>) protein levels, and representative immunoblots (<b>D</b>) in Sham and MI groups. Correlation between plantaris <i>BNIP3</i> mRNA levels and distance run in a graded treadmill exercise test (<b>E</b>, Sham n = 8, MI n = 10). Data presented as mean ± SEM. *indicates p≤0.05 vs. Sham. The number of animals in each analysis is shown within the bars.</p

Autophagic marker in soleus muscle.

<p>Soleus LC3-I (<b>A</b>) and LC3-II (<b>B</b>) protein levels, LC3-II/LC3-I ratio (<b>C</b>) and representative imunnoblots (<b>D</b>) in Sham and MI groups. Correlation between soleus LC3-II protein expression and distance run in a graded treadmill exercise test (<b>E</b>, Sham n = 9, MI n = 11). Data presented as mean ± SEM. AU, arbitrary unit. The number of animals in each analysis is shown within the bars.</p

Soleus muscle mitochondrial content.

<p>Soleus mitochondrial complexes I (<b>A</b>), III (<b>B</b>) and V (<b>C</b>) protein levels, IDH2/VDAC ratio (<b>D</b>) and representative immunoblots (<b>E</b>) in Sham and MI groups. Data presented as mean ± SEM. The number of animals in each analysis is shown in the bar.</p