Optimized Synthesis of Gold Nanoparticles using Green Chemical Process and its Invitro Anticancer Activity Against HepG2 and A549 Cell Lines

International audienceIn the present study, the biosynthesis of gold nanoparticles (AuNPs) was achieved by using marine brown seaweed Padina tetrastromatica (PT) as a reducing and capping agent. The optimized Au Nps were performed by changing the concentration of algae extract, pH and temperature was analyzed by UV-vis spectrophotometer. The synthesized Au NPs were characterized byXRD, FTIR, SEM, TEM, EDX and SAED. The X-ray diffraction showed the Au NPs which can state by the presence of peaks at (1 1 1), (2 0 0), (2 2 0) and (2 2 2). The FTIR result clearly showed that the extracts containing-OH as a functional group (sugar molecules) act in capping the nanoparticles synthesis. SEM images revealed that all particles were spherical in shape. TEM image confirms the spherical in shape with an average size ranges from 8-10 nm. Synthesized Au NPs were evaluate for the in vitro cytotoxic activity of human liver cancer (HepG2) and lung cancer (A549) cell line at the different concentrations compared with standard drug cyclophosphamide

Biosynthesis and Antimicrobial Activity of Semiconductor Nanoparticles against Oral Pathogens

Dental care is an essential phenomenon in human health. Oral pathogens can cause severe break which may show the way to serious issues in human disease like blood circulation and coronary disease. In the current study, we demonstrated the synthesis and antimicrobial activity of cadmium sulphide and zinc sulphide nanoparticles against oral pathogens. The process for the synthesis of cadmium sulphide (CdS) and zinc sulphide (ZnS) nanoparticles is fast, novel, and ecofriendly. Formation of cadmium sulphide (CdS) and zinc sulphide (ZnS) nanoparticles was confirmed by surface plasmon spectra using UV-Vis spectrophotometer. The morphology of crystalline phase of nanoparticles was determined from transmission electron microscopy (TEM) and X-ray diffraction (XRD) spectra. The average size of cadmium sulphide (CdS) and zinc sulphide (ZnS) nanoparticles was in the range of 10 nm to 25 nm and 65 nm, respectively, and the observed morphology was spherical. The results indicated that the proteins, which contain amine groups, played a reducing and controlling responsibility during the formation of cadmium sulphide (CdS) and zinc sulphide (ZnS) nanoparticles in the colloidal solution. The antimicrobial activity was assessed against oral pathogens such as Streptococcus sp. Staphylococcus sp. Lactobacillus sp., and Candida albicans and these results confirmed that the sulphide nanoparticles are exhibiting good bactericidal activity

Biosynthesis of silver nanoparticles and their versatile antimicrobial properties

Silver nanoparticles (AgNP) have been used for over a century for many purposes including as germicides. Unique physical, chemical and biological properties of Ag NPs make them suitable for a wide range of applications in different industries and biomedical fields. Green nanobiotechnology with synthesis of NPs using biomolecules (protein, enzyme, DNAand plant extracts) have become a rapidly developing research area. Green synthesis methods have overcome the disadvantages of traditional physical and chemical synthesis approaches, such as high cost, long time scales and toxicity. In the green route, the biomolecules act as both reducing and/or stabilizing agents to produce biocompatible NPs. Promising results on antimicrobial activity of AgNP against several pathogenic microorganisms have been reported in literature. The growth of multiple antibiotic resistant bacteria could be inhibited by using Ag NP. This review mainly discusses the synthesis routes and characterization of biomolecules capped Ag NPs and their enhanced antimicrobial properties towards various human and plant pathogens