Reactivity of <i>flaviviruses</i>, <i>phlebovirus</i> and <i>alphaviruses</i> in the CHIKV RT-RPA assay using two different primers pairs.

<p>The RF2+RR2 detected only the CHIKV, but not other viruses. While RF+RR3 detected both CHIKV and O'nyong'nyong virus.</p

Probit regression analyses of RT-RPA assays using two primer combinations.

<p>(A) RF+RR3 and (B) RF2+RR2. The data sets of eight RT-RPA assay runs using serial dilutions of <i>in vitro</i> transcribed nsP1 RNA ranging from 10⁷ to 10¹ RNA molecules were included in the analysis. Triangle sensitivity value at 95% probability.</p

List of the oligonucleotides of the RT-RPA and real-time RT-PCR assays.

<p>List of the oligonucleotides of the RT-RPA and real-time RT-PCR assays.</p

Results of screening clinical samples using real-time RT-RPA and RT-PCR assays.

<p>Thirty-six samples were positive in both assays with 100% agreement. Twenty-nine dots are shown as seven spots overlap. No correlation was found between RT-PCR and RT-RPA values (R = 0.2).</p

Real-Time RT-RPA run with different combinations of the designed primers.

<p>Four primer combinations were tested with the LR isolate (P): RF+RR2, RF2+RR2, RF+RR3 or RF2+RR3. The negative control (N) was water. The RF+RR3 yielded the best RPA fluorescence signal.</p

List of the 18 isolates representing the three CHIKV genotypes.

<p>All isolates were screened with real-time RT-PCR assays and RT-RPA.</p