Scotopic a-wave characteristic in WT and <i>Fmr1</i> KO mice.

<p>Retinal function was evaluated using to ElectroRetinoGram (ERG) (n = 20 for WT and n = 17 for <i>Fmr1</i> KO). For each (<b>A</b>) typical ERG obtained at light intensity −2.88 log(cd.s.m⁻²), the decreasing part of the a-wave is fitted to calculate the extrapolated (<b>B</b>) maximal a-wave amplitude (<i>A</i>_max) and (<b>C</b>) S</p><p>_A</p> parameter reflecting the photoreceptor sensitivity. In <i>Fmr1</i> KO mice compared to WT mice, <i>A</i>_max is significantly decreased by 26% (p<0.05), and the S<p>_A</p> parameter is not significantly different (p = 0.08). (<b>D</b>) a-wave latency was not different between groups. Data are presented as Mean ± SEM. Student’s t test, *p<p></p

<i>Fmr1/</i>Fmrp expression in WT and <i>Fmr1</i> KO retinas.

<p>(<b>A</b>) <i>Fmr1</i> mRNA expression was quantified by qPCR (n = 8 per group). Data are expressed as 2^−ΔCt values and normalized to 18S RNA internal control. Significant expression of <i>Fmr1</i> was only found in WT retina. (<b>B</b>) Fmrp expression was assessed by Western-blot analysis (n = 8 per group). All protein variations were normalized to β-actin. Fmrp was present in WT retinas whereas no detectable protein was observed in <i>Fmr1</i> KO. Data are presented as Mean ± SEM. Three independent experiments were performed with similar results. Student’s t test, ****p</p