2 research outputs found
Development of Betalain Producing Callus Lines from Colored Quinoa Varieties (<i>Chenopodium quinoa</i> Willd)
Betalains
are water-soluble plant pigments of hydrophilic nature
with promising bioactive potential. Among the scarce edible sources
of betalains is the grain crop quinoa (<i>Chenopodium quinoa</i> Willd), with violet, red, and yellow grains being colored by these
pigments. In this work, callus cultures have been developed from differently
colored plant varieties. Stable callus lines exhibited color and pigment
production when maintained on Murashige and Skoog medium supplemented
with the plant growth regulators 6-benzylaminopurine (8.88 μM)
and 2,4-dichlorophenoxyacetic acid (6.79 μM) with a reduction
of the nitrogen source to 5.91 mM. Pigment analysis by HPLC-DAD and
ESI-MS/MS fully describes the content of individual pigments in the
cell lines and allows the first report on the pigments present in
quinoa seedlings. Phyllocactin and vulgaxanthin I are described as
novel pigments in the species and show the potential of <i>C.
quinoa</i> culture lines in the production of compounds of nutritional
value