Presentation of the 7 kits used in the study for detecting viruses (see text for correspondence between letters and commercial denominations).

<p>Yes: group of pathogens detected; w diff: with differentiation between types. No: group of pathogens not detected.</p

Performances of the 6 kits evaluated in this study for a panel of 9 viruses or groups of viruses with reference to the combination of duplex PCR tests (Argene/bioMérieux) used as gold standard.

a<p>Kit having no probe for the detection of HKU1 coronavirus.</p>b<p>Kit having a probe for detecting 229E coronavirus only.</p>c<p>All the tested kits did not detect all the coronavirus types (see notes ^a and ^b).</p

Practicability of each kit using the NUCLISENS easyMAG as extraction instrument (see text for correspondence between letters and commercial denominations).

<p>Practicability of each kit using the NUCLISENS easyMAG as extraction instrument (see text for correspondence between letters and commercial denominations).</p

List of pathogens that were considered to be present in the panel of the 88 specimens according the combination of duplex PCR tests (Argene/bioMérieux) used as gold standard.

a<p>type 1 (n = 2), type 3 (n = 3) and type 4 (n = 5).</p>b<p>type NL63 (n = 6), type OC43 (n = 8), type 229E (n = 1), type HKU1 (n = 6) and untyped (n = 4).</p

Schematic synopsis of the design of the study.

<p>(See text for correspondence between letters and commercial denominations).</p

Workflow of the macro created for OSN counting.

The different steps of the macro created from the Fiji/imageJ software are shown in the table above. The images (A1 to A6) below are there to help better understand the table. A3: Yellow arrows indicate particle separation. A4: Red arrows indicate the particles with a surface area 2 that are excluded for counting. *The manual inputs include 3 steps: 1.2, 2.1 and 3.1. At completion, ‘OSNs counting’ generates a data table containing the number of green labelled OSNs, the number of red labelled OSNs and the number of colocalized OSNs (A6).</p