Additional file 3: Figure S2. of Functional analysis of androgen receptor mutations that confer anti-androgen resistance identified in circulating cell-free DNA from prostate cancer patients

Activation of wild-type AR by dihydrotestosterone (DHT), progesterone, estradiol, and hydrocortisone. The graphs represent the average ± SEM of three independent experiments with four replicates each. (TIF 252 kb

Additional file 1: Table S1. of Functional analysis of androgen receptor mutations that confer anti-androgen resistance identified in circulating cell-free DNA from prostate cancer patients

The results of the validation of a subset of detected mutations on MiSeq, Illumina. The run was designed to test 23 mutations in 11 cfDNA samples (both amplified and non-amplified). WGA samples are marked with *, n/a – sample not sequenced on MiSeq. Only two calls were not supported on MiSeq. S889G call in VC-012-t1 unamplified cfDNA was not detected on original 454 run, or on MiSeq resequencing. (DOCX 23 kb