Mating competitiveness experiment 2 ♂/1 ♀.

<p>For total replicates, the RIDL and ROCK controls and the actual competition experiment (RIDL vs. ROCK), values are given for the number of crosses ( = no. of females laying viable eggs) and for the mean number of eggs laid per female with Standard Errors (±SE). When comparing data from the total replicates, ROCK females fertilised by RIDL males laid more eggs (Mann-Witney, U = 1863, p = 0.043) whereas in the competition experiment (RIDL vs. ROCK), there were fewer observed fertilisations by RIDL males than expected (χ² test, χ² = 6.75, df = 1, p = 0.009).</p

Mating competitiveness experiment 10 ♂/5 ♀.

<p>This dataset includes 3 replicates of 6 ROCK/4 RIDL and 4 ROCK/6 RIDL. For transgenic and non transgenic crosses: number of crosses ( = no. of females laying viable eggs), mean no. of eggs laid per female with Standard Error (±SE), total no. of emerging males and females and mean emergence rate (no. of adults/no. of larvae) with Standard Error (±SE). There was no significant difference in the number of eggs laid by females fertilised either by RIDL or ROCK males (Mann-Witney, U = 4402, p = 0. 41).</p

Lifespan of RIDL and ROCK males.

<p>Proportions of <i>Ae. aegypti</i> males surviving when (i) 150 males (RIDL or ROCK) were caged alone (grey lines) or (ii) when 30 males (RIDL or ROCK) were caged with 120 ROCK females (black lines). Data are the average of two replicates for each experiment (males with or without females). The LT₅₀ values ( = median longevity values) were 39 for ROCK males alone, 32 for RIDL males alone, 11 for ROCK males caged with females and 9 for RIDL males caged with females. All mosquitoes were maintained off-TET.</p

Vertical transmission of Chikungunya virus in <i>Ae. aegypti</i> as determined by measuring log₁₀ RNA copies/pool of larvae and adults by real time RT-PCR.

<p>Horizontal line represent mean value for each group. Asterisks are indicating significant differences between the two groups (**p<0.01).</p

Egg hatch rate for different proportions of ROCK and RIDL males.

<p>For each ratio of strains (ROCK/RIDL): mean hatching rate (no. of larvae/no. of eggs, white bars) and mean emergence rate (no. of adults/no. of larvae, black bars) with Standard Errors (±SE). Above the figure, values (n) indicate the no. of females laying viable eggs.</p

Additional file 4: Figure S4. of Efficacy of ULV and thermal aerosols of deltamethrin for control of Aedes albopictus in Nice, France

Results of the GLMM binomial analysis of the influence of the treatments on parity rates of the natural population of Aedes albopictus in Nice. (TIF 5172 kb

Additional file 8: Table S4. of Efficacy of ULV and thermal aerosols of deltamethrin for control of Aedes albopictus in Nice, France

Results of the GLMM with negative binomial distribution analysis of the influence of the treatment on parity rate. The dependent variable is parity rate and the independent variable pre-post treatment. (DOCX 15 kb

Additional file 5: Table S1. of Efficacy of ULV and thermal aerosols of deltamethrin for control of Aedes albopictus in Nice, France

Biological interpretation of the variables and results of the GLMM analysis. We used the model with interactions to explain the effect of the treatment. (DOCX 11 kb

Additional file 3: Figure S3. of Efficacy of ULV and thermal aerosols of deltamethrin for control of Aedes albopictus in Nice, France

Route of the fogging at zone A, Les Terrasses de Saint Julien. (TIF 400 kb

Goodness-of-fit assessment for the forecasting model applied to the validation and calibration sites, based on 100 bootstrap model projections.

<p>a) according to the monitoring week of the year and b) according to the week of model initialization.</p