<i>C</i>. <i>elegans</i> spliceosomal genes showing similarity to <i>H</i>. <i>schachtii</i> transcripts and their similarity scores for genes of seven other PPNs.

<p><i>C</i>. <i>elegans</i> spliceosomal genes showing similarity to <i>H</i>. <i>schachtii</i> transcripts and their similarity scores for genes of seven other PPNs.</p

<i>H</i>. <i>schachtii</i> transcripts putatively encoding CAZymes similar to those of plant parasitic nematodes.

<p><i>H</i>. <i>schachtii</i> transcripts putatively encoding CAZymes similar to those of plant parasitic nematodes.</p

Comparative analysis of <i>H</i>. <i>schachtii</i> transcripts with genomes of four PPNs.

<p>A). Percentage of <i>H</i>. <i>schachtii</i> transcripts with matches to genomic contigs of <i>H</i>. <i>glycines</i>, <i>G</i>. <i>pallida</i>, <i>M</i>. <i>incognita</i> and <i>M</i>. <i>hapla</i>. B). Percentage of genomic contigs of <i>H</i>. <i>glycines</i>, <i>G</i>. <i>pallida</i>, <i>M</i>. <i>incognita</i> and <i>M</i>. <i>hapla</i> with similarity to <i>H</i>. <i>schachtii</i> transcripts. C). Comparison of homologues of CEGs in the <i>H</i>. <i>schachtii</i> transcriptomes to those in genomes of <i>H</i>. <i>glycines</i>, <i>G</i>. <i>pallida</i>, <i>M</i>. <i>incognita</i> and <i>M</i>. <i>hapla</i>.</p

Distribution of <i>H</i>. <i>schachtii</i> transcripts amongst genes/ESTs of nematodes of different lifestyles.

<p>Distribution of <i>H</i>. <i>schachtii</i> transcripts amongst genes/ESTs of nematodes of different lifestyles.</p

Life cycle of the beet cyst nematode, <i>Heterodera schachtii</i>.

<p>Life cycle of the beet cyst nematode, <i>Heterodera schachtii</i>.</p

Alignment of a <i>H</i>. <i>schachtii</i> contig and virus protein showing two domains with significant similarity.

<p>Alignment of a <i>H</i>. <i>schachtii</i> contig and virus protein showing two domains with significant similarity.</p

<i>H</i>. <i>schachtii</i> transcripts similar to those encoding parasitism effectors of plant parasitic nematodes.

<p><i>H</i>. <i>schachtii</i> transcripts similar to those encoding parasitism effectors of plant parasitic nematodes.</p

Reads and assembly data of the <i>H</i>. <i>schachtii</i> transcriptome.

<p>A). Size distribution of contigs assembled with CAP3 and CLC Genomics Workbench 7.0.4. B). Size distribution of singletons after read assembly with CAP3 and CLC Genomics Workbench 7.0.4. C). Transcriptome and assembly statistics of CAP3 and CLC Genomics Workbench 7.0.4.</p

Concordance of molecular serotyping results of pneumococcal control strains.

<p>^armPCR: real-time Multiplex PCR.</p><p>N/A* = serotype not included in the rmPCR panel.</p><p>No ID ^b. = sequence identity of ≤98% with sequences in GenBank.</p><p>Neg^c = negative sequetyping PCR result.</p><p>^dThe Sequetyping assay mistyped serotype 18C as 18B.</p><p>Concordance of molecular serotyping results of pneumococcal control strains.</p

Similarity of 16F-like capsular polysaccharide (<i>cps</i>) gene loci.

<p>Sequences from pneumococci serotyped as 16F Quellung but sequetyped as 9V was compared to reference 9V (CR931648) and 16F (CR931668) <i>cps</i> sequences. Artemis Comparison Tool (ACT) was used to generate and view gene homology. The top lines represent the forward and reverse strand of a serotype 9v reference, the middle lines represent the queried 16F strain and the bottom lines shows the 16F reference. The portion of the <i>wzh</i> gene that is amplified by the sequetyping assay is shown by the blue rectangle. The clear blocks below the blue box shows regions were the genes that are not similar. BLASTN matches are shown as red bands between sequences, indicating the degree of similarity between the sequences.</p