Additional file 2: Tables S1a and S1b. of Increased expression of the PI3K catalytic subunit p110δ underlies elevated S6 phosphorylation and protein synthesis in an individual with autism from a multiplex family

Complete list of p values for all paired comparisons made in Figs. 1 and 2. a List of p values of Dunnett’s post hoc analyses of each AGRE cell line compared to the healthy control. Shown are values for post hoc analyses for total S6, phosphorylated S6, and phospho-S6/S6 ratios. b List of false discovery rate-corrected p values of pairwise comparisons of phospho-S6/S6 ratios of SSC cell lines. Highlighted in yellow are comparisons with p < 0.05, which were considered significantly different. (DOC 66 kb

Additional file 3: Figure S2. of Increased expression of the PI3K catalytic subunit p110δ underlies elevated S6 phosphorylation and protein synthesis in an individual with autism from a multiplex family

Protein expression levels of PI3K catalytic subunits p110α (a), p110β (b), and p110δ (c) are unchanged in cell lines S1 and S11 compared to their healthy siblings. Representative Western blots are shown on the top, and densitometric quantifications of three to four separate experiments are on the bottom (paired t tests, a, S1: n = 4, t(3) = 1.75, p = 0.178; S11: n = 3, t(2) = 0.60, p = 0.607; b, S1: n = 4, t(3) = 1.79, p = 0.171; S11: n = 4, t(3) = 2.09, p = 0.128; c, S1: n = 4, t(3) = 0.79, p = 0.487; S11: n = 4, t(3) = 1.36, p = 0.266). Shown are means + SEM. (TIF 376 kb

Additional file 4: Tables S2a and S2b. of Increased expression of the PI3K catalytic subunit p110δ underlies elevated S6 phosphorylation and protein synthesis in an individual with autism from a multiplex family

a, p values of Dunnett’s post hoc analyses of p110δ protein expression analyses of the multiplex family shown in Fig. 5b. b, p values of Dunnett’s post hoc analyses of phospho-S6/S6 ratios measured by ELISA of the multiplex family shown in Fig. 5c. All individual family members were compared to the unaffected sister A4-S. (DOC 7 kb

Correlation between age and responding latencies elicited by left finger movement.

<p>Two charts show the statistical correlation between age and latencies of movement-evoked magnetic fields (MEFs) from left finger movement. There are positive correlations between age and the latencies of MEFs in healthy controls. However, there is no significant correlation between age and the latencies of MEFs in children with migraine. The Y-axes are latencies of MEFs in milliseconds (ms); the X-axes are ages of children in years (Years). “ML” indicates children with migraine with left finger movement; “NL” indicates health controls with left finger movement. “Rn” indicates the correlation in health controls; “Rm” indicates the correlation in children with migraine.</p

Sound-cue finger tapping task.

<p>A tone is sent to the participant’s left or right ear in a randomized order: The participant is instructed to press a button on her/his left side when the tone is sent to the left ear; the participant is instructed to press a button on her/his right side when the tone is sent to the right ear. Each button will send a unique signal to the MEG system in real-time and the MEG system will record and store the unique signals to the MEG dataset for analysis of movement-related neuromagnetic responses.</p

Magnetic source activation of high-gamma oscillations elicited by left finger movement.

<p>Magnetic Source Imaging (MSI), the combination of magnetoencephalography (MEG) results and magnetic resonance imaging (MRI), shows the source activation elicited by left finger movement in both children with migraine and healthy controls. The red and yellow areas indicate regions of neuromagnetic activation (or synchronized neural firing). The neuromagnetic activation elicited by left finger movement is localized in the contralateral motor cortex in healthy controls (“Normal”). The neuromagnetic activation elicited by left finger movement is localized in the contralateral motor cortex as well as the premotor (“16 Y”) and supplementary (“12 Y”) motor areas in children with migraine (“Migraine”).</p

The most significant regression modeling equations for auditory evoked responses to binaural tone stimulation.

*<p><i>p<0.001</i>.</p

Magnetic source activation of high-gamma oscillations elicited by right finger movement.

<p>Magnetic Source Imaging (MSI), the combination of magnetoencephalography (MEG) results and magnetic resonance imaging (MRI), shows the source activation elicited by right finger movement in both children with migraine and healthy controls. The red and yellow areas indicate regions of neuromagnetic activation (or synchronized neural firing). The neuromagnetic activation elicited by right finger movement is localized in the contralateral motor cortex in healthy controls (“Normal”). The neuromagnetic activation elicited by right finger movement is localized in the contralateral motor cortex as well as the premotor and supplementary motor areas in children with migraine (“Migraine”).</p

Scatter plot of the peak latencies of the M50, M70 and M100 components in the left and right auditory hemispheres relative to chronological age of the children.

<p>Linear regression lines, using the least-squares method, are also plotted to show general trends for each hemisphere. The M70 components show a clear developmental change with the highest correlation coefficient.</p

Demographic and Clinical Features of Children with Migraine.

<p>Demographic and Clinical Features of Children with Migraine.</p