Flow cytometry analysis details and representative cytograms in a CM patient.

<p>MP were discriminated by their size and structures (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0013415#pone-0013415-g001" target="_blank">Fig. <b>1A</b></a>). And events in MP gate were further analyzed to differentiate Annexin V⁺ MP from the background signal. An internal standard, constituted of fluorosphere beads of a known size and at a known concentration was used to help set the MP gate and calculate the MP level. Representative cytograms: <b>1B</b> to <b>1H</b> Illustrations of the differences in MP positive events in cytograms representing total MP and cell-derived MP in a CM patient. (<b>B</b>): T-MP:annexin V⁺; (<b>C</b>): P-MP:CD41⁺; (<b>D</b>): E-MP:CD105⁺; (<b>E</b>): R-MP:CD235a⁺; (<b>F</b>): E-MP:CD51⁺; (<b>G</b>): M-MP:CD11b⁺; (<b>H</b>): L-MP:CD3⁺.These MP positive events were used in the calculations of the number of MP/µl of plasma (see the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0013415#s2" target="_blank">methods</a> section).</p

Correlations between MP and some clinical/biological parameters.

<p>A to E: correlations between P-MP numbers and temperature, fever resolution time (FRT), Blantyre coma score (BCS), coma resolution time (CRT) and platelet counts, respectively. F: correlation between R-MP and hemoglobin levels (Hgb).</p

Total and cellular MP levels per µl of plasma at follow-up/discharge (7 days after admission:

<p><i>A</i>) versus levels on admission. (<b>A</b>): T-MP:annexin V⁺; (<b>B</b>): P-MP:CD41⁺; (<b>C</b>): E-MP:CD105⁺; (<b>D</b>): R-MP:CD235a⁺; (<b>E</b>): M-MP:CD11b⁺; (<b>F</b>): L-MP:CD3⁺. Dot plot representations showing how in most of the cases, MP levels decreases in patients with CM at discharge (<i>D</i>). Control group is represented to show that patient levels are returning to control levels. C: Controls; SA: severe malaria anemia patients on admission; CM: cerebral malaria patients on admission; CM+SA: patients with the combine symptoms of CM and SMA on admission; SA(D): severe malaria anemia patients at discharge; CM(D): cerebral malaria patients at discharge; CM+SA(D): patients with the combine symptoms of CM and SMA at discharge; MP levels are given in MP/µl of plasma. The values of the deceased patients are presented as black stars on <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0013415#pone-0013415-g003" target="_blank">Figure 3</a>. *: p<0.05, **: p<0.001, ***: p<0.0001.</p