In ovo time-lapse analysis after dorsal neural tube ablation shows rerouting of chick hindbrain neural crest

Previous analyses of single neural crest cell trajectories have suggested important roles for interactions between neural crest cells and the environment, and amongst neural crest cells. To test the relative contribution of intrinsic versus extrinsic information in guiding cells to their appropriate sites, we ablated subpopulations of premigratory chick hindbrain neural crest and followed the remaining neural crest cells over time using a new in ovo imaging technique. Neural crest cell migratory behaviors are dramatically different in ablated compared with unoperated embryos. Deviations from normal migration appear either shortly after cells emerge from the neural tube or en route to the branchial arches, areas where cell-cell interactions typically occur between neural crest cells in normal embryos. Unlike the persistent, directed trajectories in normal embryos, neural crest cells frequently change direction and move somewhat chaotically after ablation. In addition, the migration of neural crest cells in collective chains, commonly observed in normal embryos, was severely disrupted. Hindbrain neural crest cells have the capacity to reroute their migratory pathways and thus compensate for missing neural crest cells after ablation of neighboring populations. Because the alterations in neural crest cell migration are most dramatic in regions that would normally foster cell-cell interactions, the trajectories reported here argue that cell-cell interactions have a key role in the shaping of the neural crest migration

In ovo time-lapse analysis of chick hindbrain neural crest cell migration shows cell interactions during migration to the branchial arches

Hindbrain neural crest cells were labeled with DiI and followed in ovo using a new approach for long-term time-lapse confocal microscopy. In ovo imaging allowed us to visualize neural crest cell migration 2-3 times longer than in whole embryo explant cultures, providing a more complete picture of the dynamics of cell migration from emergence at the dorsal midline to entry into the branchial arches. There were aspects of the in ovo neural crest cell migration patterning which were new and different. Surprisingly, there was contact between neural crest cell migration streams bound for different branchial arches. This cell-cell contact occurred in the region lateral to the otic vesicle, where neural crest cells within the distinct streams diverted from their migration pathways into the branchial arches and instead migrated around the otic vesicle to establish a contact between streams. Some individual neural crest cells did appear to cross between the streams, but there was no widespread mixing. Analysis of individual cell trajectories showed that neural crest cells emerge from all rhombomeres (r) and sort into distinct exiting streams adjacent to the even-numbered rhombomeres. Neural crest cell migration behaviors resembled the wide diversity seen in whole embryo chick explants, including chain-like cell arrangements; however, average in ovo cell speeds are as much as 70% faster. To test to what extent neural crest cells from adjoining rhombomeres mix along migration routes and within the branchial arches, separate groups of premigratory neural crest cells were labeled with DiI or DiD. Results showed that r6 and r7 neural crest cells migrated to the same spatial location within the fourth branchial arch. The diversity of migration behaviors suggests that no single mechanism guides in ovo hindbrain neural crest cell migration into the branchial arches. The cell-cell contact between migration streams and the co-localization of neural crest cells from adjoining rhombomeres within a single branchial arch support the notion that the pattern of hindbrain neural crest cell migration emerges dynamically with cell-cell communication playing an important guidance role

Evaluation of Lumicyano cyanoacrylate fuming process for the development of latent fingermarks on plastic carrier bags by means of a pseudo operational comparative trial

There are a number of studies discussing recent developments of a one-step fluorescent cyanoacrylate process. This study is a pseudo operational trial to compare an example of a one-step fluorescent cyanoacrylate product, Lumicyano™, with the two recommended techniques for plastic carrier bags; cyanoacrylate fuming followed by basic yellow 40 (BY40) dyeing and powder suspensions. 100 plastic carrier bags were collected from the place of work and the items were treated as found without any additional fingermark deposition. The bags were split into three and after treatment with the three techniques a comparable number of fingermarks were detected by each technique (average of 300 fingermarks). The items treated with Lumicyano™ were sequentially processed with BY40 and an additional 43 new fingermarks were detected. Lumicyano™ appears to be a suitable technique for the development of fingermarks on plastic carrier bags and it can help save lab space and time as it does not require dyeing or drying procedures. Furthermore, contrary to other one-step cyanoacrylate products, existing cyanoacrylate cabinets do not require any modification for the treatment of articles with Lumicyano™. To date, there is little peer reviewed articles in the literature on trials related to Lumicyano™ and this study aims to contribute to fill this gap

Pseudo-operational trials of Lumicyano solution and Lumicyano powder for the detection of latent fingermarks on various substrates

This study presents pseudo-operational trials comparing a one-step fluorescent cyanoacrylate process with a number of other enhancement techniques on a variety of substrates. This one-step process involves a product, 4% Lumicyano, which is a solution consisting of 4% by weight of a powdered dye (Lumicyano powder) dissolved in a cyanoacrylate-based solution (Lumicyano solution). The cyanoacrylate in the Lumicyano solution may be of a higher quality than that used in the two-step products.One hundred items were collected from the place of work for each trial. Trial 1 involved a comparison of 4% Lumicyano with the conventional two-step cyanoacrylate fuming-dye staining for the detection of latent fingermarks on plastic carrier bags. Trial 2 assessed the quality of the Lumicyano solution (with no powdered dye) but used in a two-step process with basic yellow 40 (BY40). Trial 1, using 4% Lumicyano powder and traditional cyanoacrylate → BY40 detected a similar amount of fingermarks (~295); however, sequential BY40 treatment (i.e., after 4% Lumicyano) detected an additional 30% marks. Trial 2 resulted in the detection of 565 marks after Lumicyano solution → BY40 in comparison to 489 marks after traditional cyanoacrylate fuming and BY40 staining. Trials 3 through 5 compared 4% Lumicyano, 1,2-indanedione-zinc, and ninhydrin on junk mail, magazines, and cardboard used for food or cosmetic packaging; the detection rate was low for all techniques and substrates. Trial 6 on cardboard packaging using 4% Lumicyano, black iron-oxide powder suspension, and magnetic powder also provided a low detection rate. Trial 7, using 4% Lumicyano → BY40, solvent black 3, and iron-oxide powder suspensions on cardboard packaging from a fast food chain, indicated that 4% Lumicyano → BY40 might be a suitable alternative to solvent black 3 and iron-oxide powder suspensions for suspected greasy marks

A comparison of the use of vacuum metal deposition versus cyanoacrylate fuming for visualisation of fingermarks and grab impressions on fabrics

Both vacuum metal deposition (VMD) and cyanoacrylate fuming (CAF) are techniques used to visualise latent fingermarks on smooth non-porous surfaces such as plastic and glass. VMD was initially investigated in the 1970s as to its effectiveness for visualising prints on fabrics, but was abandoned when radioactive sulphur dioxide was found to be more effective. However, interest in VMD was resurrected in the 1990s when CAF was also used routinely. We now report on studies to determine whether VMD or CAF is the more effective technique for the detection of marks on fabrics. Four different fabrics, nylon, polyester, polycotton and cotton, were utilised during this study, along with 15 donors who ranged in their age and ability to leave fingermarks, from good to medium to poor, thus reflecting the general population. Once samples were collected they were kept for a determined time (1, 2, 3, 4, 5, 6, 7, 14, 21 or 28 days) and then treated using either the gold and zinc metal VMD process or standard cyanoacrylate fuming.The smoother fabrics, such as nylon, consistently produced greater ridge detail whereas duller fabrics, like cotton tended only to show empty prints and impressions of where the fabric had been touched, rather than any ridge details. The majority of fabrics did however allow the development of touch marks that could be targeted for DNA taping which potentially could lead to a DNA profile. Of the two techniques VMD was around 5 times more effective than CAF, producing a greater amount of ridge detail, palmar flexion creases and target areas on more samples and fabrics

Life History and Habitat Use of the Juvenile Alabama Shad (\u3ci\u3eAlosa alabamae\u3c/i\u3e) in Northern Gulf of Mexico Rivers

The Alabama shad, Alosa alabamae, is an anadromous species that is in decline and has seen extirpations from impoundments as well as decreased water quality. Alabama shad live in the Gulf of Mexico and ascend Northern Gulf of Mexico Drainages to reproduce early in the year (January-May). The juveniles spend the majority of the year in these freshwater systems before emigrating out to the Gulf of Mexico as late as December. This dissertation focuses on the juvenile life stages that occur within the Northern Gulf of Mexico drainages. Spawning conditions of the river, as well as the habitat and diet, are studied and documented. Habitat use of the juvenile Alabama shad shifted from sand bars to open channel and banks which was seen in progression as the juveniles increased in size and maturity. Important physicochemical parameters that influenced the presence of Alabama shad within these habitats ranged from temperature, flow velocity, and conductivity. Diet of the juvenile Alabama shad consisted of particulate detritus within Alabama shadtemperatures. These findings suggest that the management of these drainages should be done independently on a drainage-by-drainage basis. Habitats are consistent between drainages, but the physicochemical factors driving the presence of this species are unique to each drainage. It is also suggested that maintaining natural flows for habitat maintenance and reproductive cues is important toward conservation of the species

Distinct modes of floor plate induction in the chick embryo

To begin to reconcile models of floor plate formation in the vertebrate neural tube, we have performed experiments aimed at understanding the development of the early floor plate in the chick embryo. Using real-time analyses of cell behaviour, we provide evidence that the principal contributor to the early neural midline, the future anterior floor plate, exists as a separate population of floor plate precursor cells in the epiblast of the gastrula stage embryo, and does not share a lineage with axial mesoderm. Analysis of the tissue interactions associated with differentiation of these cells to a floor plate fate reveals a role for the nascent prechordal mesoderm, indicating that more than one inductive event is associated with floor plate formation along the length of the neuraxis. We show that Nr1, a chick nodal homologue, is expressed in the nascent prechordal mesoderm and we provide evidence that Nodal signalling can cooperate with Shh to induce the epiblast precursors to a floor-plate fate. These results indicate that a shared lineage with axial mesoderm cells is not a pre-requisite for floor plate differentiation and suggest parallels between the development of the floor plate in amniote and anamniote embryos

Robotic Astronomy with the Faulkes Telescopes and Las Cumbres Observatory Global Telescope

We present results from ongoing science projects conducted by members of the Faulkes Telescope (FT) team and Las Cumbres Observatory Global Telescope (LCOGT). Many of these projects incorporate observations carried out and analysed by FT users, comprising amateur astronomers and schools. We also discuss plans for the further development of the LCOGT network.Comment: 8 pages, 2 figures, conference proceedings from "Workshop on Robotic Autonomous Observatories", held at Malaga, Spain from 18-21 May 2009, acccepted for publication in Advances in Astronom