Photomicrographs showing p-SMAD1/5/8 immunoreactivity.

<p>Photomicrographs showing similar p-SMAD1/5/8 immunoreactivity (brown DAB staining) in the endothelium of rat pulmonary vasculature in (<b>A</b>) control lung at gestation e19.5 (manification ×100) and (<b>B</b>) LCDH lung, at gestation e19.5 (×100). p-SMAD1/5/8 immunoreactivity in the airway epithelium in (<b>C</b>) control lung at gestation e21.5 (manification ×25) and (<b>D</b>) LCDH lung, at gestation e21.5 (×25).</p

Western blots and graphs showing p-ERK1/2, total ERK1/2 and actin immunoreactivity.

<p>Western blot showing p-ERK1/2, total ERK1/2 and actin immunoreactivity (<b>a</b>). p-ERK1/2 immunoreactivity relative to actin (<b>b</b>) and total ERK1/2 (<b>c</b>) in control lung (grey boxes) and LCDH lung (white boxes) expressed as median (bar) and inter-quartile ranges (box).</p

Photomicrographs showing TIE-2 immunoreactivity.

<p>Photomicrographs showing similar TIE-2 immunoreactivity (brown DAB staining) in the endothelium of rat pulmonary vasculature at gestation e20.5 in (<b>A</b>) control lung (manification ×100) and (<b>B</b>) LCDH lung (×100).</p

The experimental model.

<p>Outline of the experimental model.</p

Western blots and graphs showing ANG-1 and actin immunoreactivity.

<p>Western blots showing ANG-1 and actin immunoreactivity in control and LCDH lung (<b>a</b>). ANG-1 immunoreactivity in control (grey) and LCDH lung (white boxes) expressed as median (bar) and inter-quartile ranges (box) (<b>b</b>). Statistical analysis found a significant linear trend (#) in the fall in ANG-1.</p

Western blots and graphs showing TIE-2 and actin immunoreactivity.

<p>Western blots showing TIE-2 and actin immunoreactivity in control and LCDH lung (<b>a</b>). TIE-2 immunoreactivity in control (grey) and LCDH lung (white boxes) expressed as median (bar) and inter-quartile ranges (box) (<b>b</b>). Statistical analysis found a significant linear trend (#) in the rise in TIE-2.</p

Western blot and graph showing p-SMAD1/5/8 and actin immunoreactivity.

<p>Western blot showing p-SMAD1/5/8 and actin immunoreactivity (+ve cell = positive control cell lysate, BMP stimulated C6 cells) (<b>a</b>). p-SMAD1/5/8 immunoreactivity in control (grey) and LCDH lung (white boxes) expressed as median (bar) and inter-quartile ranges (box) (<b>b</b>).</p

Western blot and graph showing phosphorylated (p) TIE-2 immunoreactivity.

<p>Western blot showing p-TIE-2 immunoreactivity in e20.5 lung in three contempraneously prepared sample pairs, 1,2 and 3 (C = control, H = LCDH). Total TIE-2 expression in sample pair 3 is shown (*C3 & *H3) as a positive control (<b>a</b>). Normalised p-TIE-2 immunoreactivity in control (grey) and LCDH lung (white boxes) expressed as median and inter-quartile ranges (<b>b</b>).</p