Biological identification of springtails (Hexapoda: Collembola) from the Canadian Arctic, using mitochondrial DNA barcodes

We evaluated sequence diversity in the mitochondrial cytochrome-c oxidase I (COI; EC 1.9.3.1) gene as a tool for resolving differences among species of Arctic springtails. The Collembola examined in this analysis were collected from Igloolik, Cornwallis, and Somerset islands and included representatives from all major families found in the Arctic. Members of 13 genera and 19 species were examined, including 4 species of the genus Folsomia and 3 species of the genus Hypogastrura. In all cases, species were successfully discriminated. Sequence divergences within species were generally less than 1%, whereas divergences between species were greater than 8% in all cases. Divergences among individuals of one species of Folsomia were much higher (up to 13%), but this likely represents the presence of an undescribed sibling species. We conclude that DNA barcoding is a powerful tool for identifying species of Collembola and should regularly be useful as a complement to traditional, morphological taxonomy

Testing use of mitochondrial COI sequences for the identification and phylogenetic analysis of New Zealand caddisflies (Trichoptera)

We tested the hypothesis that cytochrome c oxidase subunit 1 (COI) sequences would successfully discriminate recognised species of New Zealand caddisflies. We further examined whether phylogenetic analyses, based on the COI locus, could recover currently recognised superfamilies and suborders. COI sequences were obtained from 105 individuals representing 61 species and all 16 families of Trichoptera known from New Zealand. No sequence sharing was observed between members of different species, and congeneric species showed from 2.3 to 19.5% divergence. Sequence divergence among members of a species was typically low (mean = 0.7%; range 0.0–8.5%), but two species showed intraspecific divergences in excess of 2%. Phylogenetic reconstructions based on COI were largely congruent with previous conclusions based on morphology, although the sequence data did not support placement of the purse-cased caddisflies (Hydroptilidae) within the uncased caddisflies, and, in particular, the Rhyacophiloidea. We conclude that sequence variation in the COI gene locus is an effective tool for the identification of New Zealand caddisfly species, and can provide preliminary phylogenetic inferences. Further research is needed to ascertain the significance of the few instances of high intra-specific divergence and to determine if any instances of sequence sharing will be detected with larger sample sizes

Testing surfactants as additives for clay improvement: compaction and suction effects

This paper presents an exploratory study on surfactants as additives to improve soil properties. It is hypothesized that surfactant molecules populate the air-water interfaces reducing surface tension and suction thus allowing a control of the mechanical response of the soil. Suction measurements by means of a high suction tensiometer, compaction tests and Atterberg limits were conducted in mixtures of sand and kaolin, with and without a surfactant solution. The results revealed a prominent effect on suction, but to a lesser extent on the Atterberg limits and compaction behavior (the maximum dry density). This targeted effect of the surfactants suggests its molecules populate, not only the air-water interfaces decreasing surface tension, but may be adsorbing to the clay particles and forming micelles in the pore water as well. Therefore the interplay between the three may influence the soil behavior

Performance Management: A model and research agenda

Performance Management deals with the challenge organizations face in defining, measuring and stimulating employee performance with the ultimate goal to improve organizational performance. Thus, Performance Management involves multiple levels of analysis and is clearly linked to the topics studied in strategic HRM as well as performance appraisal. This paper presents a model for Performance Management combining insights from strategic HRM and I/O psychology. The model incorporates multi-level elements, and adds to previous models by explicitly incorporating employee perceptions, the role of direct supervisors and possible reversed causality. Challenges for future research are also presented

Reflexivity in Teams: A Measure and Correlates

Reflexivity -the extent to which teams reflect upon and modify their functioning- has been identified as a possible key factor in the effectiveness of work teams. The aim of the present study was to develop a questionnaire to measure (aspects of) reflexivity, with a focus on team reflection. The questionnaire was tested in two different samples, namely a first sample of 59 teams from fourteen different organizations (Study 1) and a confirmation sample of 59 school management teams (Study 2). In both samples, two factors of reflection were identified. These were labeled evaluation/learning and discussing processes/principles. Scale statistics showed good psychometric properties for the scales in both studies. We conclude that the scales form a parsimonious and valid instrument to assess reflexivity in teams

High levels of intraspecific genetic divergences revealed for Antarctic springtails: evidence for small-scale isolation during Pleistocene glaciation

We examined levels of genetic variability within and among populations of three Antarctic springtail species (Arthropoda: Collembola) and tested the hypothesis that genetic divergences occur among glacially-isolated habitats. The study was conducted in southern Victoria Land, Ross Dependency, Antarctica, and samples were collected from locations in the vicinity of the Mackay Glacier. We analyzed mtDNA (cytochrome c oxidase subunit I; COI) sequence variability for 97 individuals representing three species (Gomphiocephalus hodgsoni, N = 67; Cryptopygus nivicolus, N = 20; and Antarcticinella monoculata, N = 8). Haplotype diversity and genetic divergences were calculated and used to indicate population variability and also to infer divergence times of isolated populations using molecular clock estimates. Two of the three species showed high levels of genetic divergence. Gomphiocephalus hodgsoni, a widespread and common species, showed 7.6% sequence divergence on opposite sides of the Mackay Glacier. The more range restricted C. nivicolus showed 4.0% divergence among populations. The third species, A. monoculata, was found in only one location. Molecular clock estimates based on sequence divergences suggest that populations separated within the last 4 Mya. We conclude that habitat fragmentation resulting from Pliocene (5 Mya) and Pleistocene (2 Mya to 10 Kya) glaciations has promoted and maintained high levels of diversity among isolated springtail populations on relatively small spatial scales. The region surrounding the Mackay Glacier is likely to have provided refugia for springtail populations during glacial maxima and remains an area of high genetic and species diversity for Collembola within the Ross Sea region

Resolving the taxonomic status of potential biocontrol agents belonging to the neglected genus lipolexis Förster (Hymenoptera, Braconidae, Aphidiinae) with descriptions of six new species

Lipolexis is a small genus in the subfamily Aphidiinae represented by one species in Europe (Lipolexis gracilis Förster) and by four in Asia (Lipolexis wuyiensis Chen, L. oregmae Gahan, L. myzakkaiae Pramanik and Raychaudhuri and L. pseudoscutellaris Pramanik and Raychaudhuri). Although L. oregmae is employed in biological control programs against pest aphids, the last morphological study on the genus was completed over 50 years ago. This study employs an integrative approach (morphology and molecular analysis (COI barcode region)), to examine Lipolexis specimens that were sampled worldwide, including specimens from BOLD database. These results establish that two currently recognized species of Lipolexis (L. gracilis, L. oregmae) are actually a species complex and also reveal phylogenetic relationships within the genus. Six new species are described and a global key for the identification of Lipolexis species is provided.info:eu-repo/semantics/publishedVersio

Analytic approximation and an improved method for computing the stress-energy of quantized scalar fields in Robertson-Walker spacetimes

An improved method is given for the computation of the stress-energy tensor of a quantized scalar field using adiabatic regularization. The method works for fields with arbitrary mass and curvature coupling in Robertson-Walker spacetimes and is particularly useful for spacetimes with compact spatial sections. For massless fields it yields an analytic approximation for the stress-energy tensor that is similar in nature to those obtained previously for massless fields in static spacetimes.Comment: RevTeX, 8 pages, no figure

Reionization: Characteristic Scales, Topology and Observability

Recently the numerical simulations of the process of reionization of the universe at z>6 have made a qualitative leap forward, reaching sufficient sizes and dynamic range to determine the characteristic scales of this process. This allowed making the first realistic predictions for a variety of observational signatures. We discuss recent results from large-scale radiative transfer and structure formation simulations on the observability of high-redshift Ly-alpha sources. We also briefly discuss the dependence of the characteristic scales and topology of the ionized and neutral patches on the reionization parameters.Comment: 4 pages, 5 figures (4 in color), to appear in Astronomy and Space Science special issue "Space Astronomy: The UV window to the Universe", proceedings of 1st NUVA Conference ``Space Astronomy: The UV window to the Universe'' in El Escorial (Spain

Non-Vacuum Bianchi Types I and V in f(R) Gravity

In a recent paper \cite{1}, we have studied the vacuum solutions of Bianchi types I and V spacetimes in the framework of metric f(R) gravity. Here we extend this work to perfect fluid solutions. For this purpose, we take stiff matter to find energy density and pressure of the universe. In particular, we find two exact solutions in each case which correspond to two models of the universe. The first solution gives a singular model while the second solution provides a non-singular model. The physical behavior of these models has been discussed using some physical quantities. Also, the function of the Ricci scalar is evaluated.Comment: 15 pages, accepted for publication in Gen. Realtiv. Gravi