Luminescence generated over time after treating a <i>B</i>. <i>cenocepacia</i> K56-2 <i>oxyR</i>:<i>lux</i> promoter fusion mutant biofilm with 0.03% H₂O₂, Cip (4 x MIC), Mer (4 x MIC) or Tob (2 x MIC) (up to 6 h) compared to luminescence in biofilms exposed to LB alone (blue).

<p>Data are shown of a single representative experiment.</p

H2DCFDA fluorescence in treated (Tob 4 x MIC, Cip 16 x MIC, Mer 16 x MIC, 24 h) versus untreated planktonic cultures of <i>B</i>. <i>multivorans</i> LMG 13010, <i>B</i>. <i>vietnamiensis</i> LMG 10929, <i>B</i>. <i>cepacia</i> LMG 1222, <i>B</i>. <i>metallica</i> LMG 24068 and <i>B</i>. <i>cenocepacia</i> K56-2.

<p>Error bars represent SEM. Statistically significant differences are indicated with an asterisk, p < 0.05, n ≥ 3.</p

Fluorescence generated over time in treated (4 x MIC, up to 13 h) (red circles) and untreated (blue squares) biofilms (left) and planktonic cultures (right).

<p>(A) Cultures incubated with (closed circles and squares) or without (open circles and squares) H2DCFDA. (B) Cultures incubated with (closed circles and squares) or without (open circles and squares) HPF. Data are shown of a single representative experiment. Error bars represent SEM (calculated on 3 technical replicates).</p

EPR determination of radicals formed in treated and untreated (blue) planktonic cultures of <i>B</i>. <i>cenocepacia</i> K56-2.

<p>Cultures were treated with Tob (4 x MIC, 30 min, red), Cip (4 x MIC, 30 min and 180 min, orange) or Mer (4 x MIC, 30 min and 180 min, green). The probe was added 30 min before measurement. Error bars represent SEM. Statistically significant differences are indicated with an asterisk, p < 0.05, n = 4.</p

Correlation between fold change in survival and fold change in fluorescence after treatment with Tob or Cip (4 x MIC, 24 h) in combination with an antioxidant compared to treatment with Tob or Cip alone.

<p>Correlation between fold change in survival and fold change in fluorescence after treatment with Tob or Cip (4 x MIC, 24 h) in combination with an antioxidant compared to treatment with Tob or Cip alone.</p

Fluorescence generated over time after treatment with Tob (4 x MIC, up to 20 h) (red), Cip (16 x MIC, up to 20 h) (orange) or Mer (16 x MIC, up to 20 h) (green) in biofilms and planktonic <i>B</i>. <i>cenocepacia</i> K56-2 cultures compared to fluorescence in pH-matched controls (blue).

<p>Cultures were pre-incubated with H2DCFDA and treated with antibiotics or control solutions with the same pH. Data are shown of a single representative experiment. Error bars represent SEM (calculated on 3 technical replicates).</p

Fluorescence generated over time in biofilms and planktonic <i>B</i>. <i>cenocepacia</i> K56-2 cultures after treatment with Tob (red) in different concentrations (4xMIC, MIC, MIC/4, up to 8 h) compared to fluorescence in pH-matched control (blue).

<p>Cultures were pre-incubated with H2DCFDA and treated with antibiotics or control solutions with the same pH. Data are shown of a single representative experiment. Error bars represent SEM (calculated on 3 technical replicates).</p