Appendix C. Assumptions involved in the determination of stable isotope composition of phytoplankton: justification and literature comparison.

Assumptions involved in the determination of stable isotope composition of phytoplankton: justification and literature comparison

Appendix A. Input data used for allochthony modeling in SIAR 4.0 for R.

Input data used for allochthony modeling in SIAR 4.0 for R

Appendix B. Validation of the assumed distribution of ω (dietary water contribution per trophic level) in zooplankton, and coupled sensitivity analysis.

Validation of the assumed distribution of ω (dietary water contribution per trophic level) in zooplankton, and coupled sensitivity analysis

The contribution of AAP bacteria to total bacterial abundance (%AAP, squares) and the BChl<i>a</i> content per cell (circles) as function of the DOC to Chl<i>a</i> (DOC:Chl<i>a</i>) ratio.

<p>The data have been log-transformed, the line is the least-square regression, and details of the regression models are given in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0124035#pone.0124035.t001" target="_blank">Table 1</a>.</p

Regression models relating AAP bacteria variables to total bacteria or describing the cross-lake patterns in AAP bacteria and total bacteria as a function of environmental variables.

<p>AAP: Aerobic anoxygenic phototrophic bacterial abundance; BA: Total bacterial abundance; Total size: Total prokaryotic cell size; BV: total biovolume; SpBChl<i>a</i>: Pigment content per cell; %BChl<i>a</i>: relative contribution of BChl<i>a</i> to total pigments; Secchi: Secchi disc depth; DOC: Dissolved organic carbon.</p><p>Regression models relating AAP bacteria variables to total bacteria or describing the cross-lake patterns in AAP bacteria and total bacteria as a function of environmental variables.</p

Seasonal patterns in the contribution of AAP bacteria to (a) total bacterial abundance and to biovolume, and (b) in the average cell size of AAP and total bacteria.

<p>The box-and-whisker plots show the average, 75% quartiles and extremes values, and stars indicate significant differences (p<0.05).</p

The relationship of total bacterial abundance (triangles) and AAP bacterial abundance (circles) with total phosphorous concentration (a), Secchi depth (b), and DOC concentration (c).

<p><b>BChl<i>a</i> contents per cell as a function of mean lake chlorophyll concentration (d)</b>. Data have been log-transformed, the line is the least-square regression, and details of the regression models are given in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0124035#pone.0124035.t001" target="_blank">Table 1</a>.</p

The average and distribution of the relative abundance of AAP bacteria (%AAP), and of the pigment content per cell volume (fg BChl<i>a</i> μm^-3) in the epilimnion and in the metalimion of selected lakes.

<p>The box-and-whisker plots show the average, 75% quartiles and extremes values in the epilimnetic and metalimnetic layers during lake summer stratification.</p

Relationship between total prokaryote abundance and (a) AAP bacterial abundance and (b) cell size.

<p><b>(c) Contribution of AAP bacteria to total abundance (%AAP) versus their contribution to total bacterial biovolume (%BV).</b> The line in (a) and (b) is the least-square regression, and details of the regression models are given in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0124035#pone.0124035.t001" target="_blank">Table 1</a>. The line in (c) is the 1:1 line. Data have been log-transformed, and the plots comprise all data collected between July 2007 and October 2008.</p

Effects of urea fertilization rate (mg N L^-1 week^-1) on mean planktonic parameters.

<p>Results averaged for days 7–21. Response variables include (a) phytoplankton biomass (as Chl <i>a</i>), (b) bacterial density, (c) gross primary production (GPP), (d) bacterial C consumption (BCC; productivity + respiration), (e) the approximate metabolic balance of plankton communities, measured as GPP: BCC, and (f) dissolved oxygen concentration (DO). Data in each panel includes July (black circles and thick black lines), August (grey triangles and grey lines), and September (white squares and thin black lines) experiments. Solid lines indicate best-fit regression models detailed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0188652#pone.0188652.t001" target="_blank">Table 1</a>, dashed lines indicate direction of change for trial in which statistically-significant regression models could not be fit. Error bars = ± 1 S.E, and <i>n</i> = 9.</p