Optimisation of germination time and temperature on the concentration of bioactive compounds in Brazilian soybean cultivar BRS 133 using response surface methodology

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)The objective was to optimise the effect of germination time and temperature on the concentration of soluble protein. lunasin, 13131, lectin, saponins and isoflavones in soybean seeds from cultivar BRS 133. Isoflavone and saponin concentrations were analysed by HPLC. Lunasin, Bowman-Birk inhibitor and lectin were analysed by ELISA and western blot. The effects of the variations in germination time and temperature on bioactive Compounds were analysed using the response surface methodology (RSM), with a 2(2) central composite rotational design. Germination of soybean for 42 h at 25 degrees C resulted in an increase of 61.7% of lunasin, decrease of 58.7% in lectin and 70.0% in lipoxygenase activity. Optimal increases in the concentrations of isoflavone aglycones were observed in combination of 63 h of germination and 30 degrees C. A significant increase of 32.2% in the concentration of soy saponins was observed in combination of 42 h of germination at 25 degrees C. (C) 2009 Elsevier Ltd. All rights reserved.1192636642Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)SoSoja do Brasil Ltda.Sun Foods-BrazilEmbrapa Technology Transfer, BrazilUnicamp's Foundation for Teaching, Research and Extension (FAEPEX) [149/08]Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Unicamp's Foundation for Teaching, Research and Extension (FAEPEX) [149/08

Effect of time and temperature on bioactive compounds in germinated Brazilian soybean cultivar BRS 258

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)The objective was to determine the effect of time and temperature on the concentration of bioactive compounds during germination of Brazilian soybean cultivar BRS 258. The concentration of bioactive compounds was optimized using Response Surface Methodology, with a 2(2) central composite rotational design and germination time and temperature as independent variables. Germination was carried out in a germination chamber, using paper in trays containing 500 g of seeds. Germination temperature and time modified the concentrations of bioactive compounds within the ranges studied. An increase in germination time at 25 degrees C decreased the concentration of Bowman-Birk inhibitor, lectin and lipoxygenase. After 63 h of germination, a temperature increase from 20 degrees C to 30 degrees C resulted in a decrease of lipoxygenase activity by 22.5%. Optimal increases in the concentrations of isoflavone aglycones (daidzein and genistein) and saponin glycosides were observed with a 63 h germination time at 30 degrees C. Both germination time and temperature had an influence on the composition and concentration of bioactive compounds in germinated soybean flour. Equations determined can be used to predict concentrations of bioactive compounds in germinated soybeans in relation to climate changes and thus optimize their potential use in human health and nutrition. (c) 2009 Elsevier Ltd. All rights reserved.437SI18561865Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES

A high-protein soybean cultivar contains lower isoflavones and saponins but higher minerals and bioactive peptides than a low-protein cultivar

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Soybean is a major source of protein and other nutrients and non-nutrient bioactives for human health. The objective was to compare the bioactive compounds of a low-protein (BRS 133) soybean in comparison to a high-protein (BRS 258) soybean cultivar. The high-protein soybean contained 17% lower carbohydrates and a lower chemical score (63) in relation to the low-protein soybean, which had a higher chemical score (76), associated with the higher methionine content (1.2%). Cultivar BRS 258 had more calcium (15.5%), phosphorus (30.1%), iron (18.7%). copper (9.0%) and zinc (11.5%), and a higher concentrations of lunasin, BBI and lectin (20.3%, 19.0% and 27.1%, respectively) than the low-protein cultivar. BRS 133 had 75.4% higher concentration of total isoflavones (5.1% of total aglycones) and 31.0% total saponins, as compared to BRS 258. It was concluded that the low-protein soybean cultivar contained higher isoflavones and saponins, but lower levels of minerals and bioactive peptides, such as lunasin. (C) 2009 Elsevier Ltd. All rights reserved.12011521Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Embrapa Soybean and Embrapa Technology Transfer, BrazilFAEPEXFoundation for Teaching, Research and Extension (Unicamp)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES