O CUIDADO EM SAÚDE E AS IMPLICAÇÕES PARA OS CUIDADORES DOMICILIARES

RESUMO: O artigo trata da temática do cuidado domiciliar em saúde, modalidade de atendimento que tem crescido em vários países, impulsionada pelas transições demográficas e epidemiológicas dos últimos anos. Com foco em duas experiências, o trabalho apresenta os resultados de uma pesquisa com o objetivo de analisar o perfil dos cuidadores de dois programas de atenção em saúde domiciliar sendo, um público e outro privado desenvolvido em cidades localizadas na Região da Grande Florianópolis. Trata-se de uma pesquisa qualitativa com aplicação de entrevistas com cuidadores de pessoas em internação domiciliar. Como principais resultados aponta-se que a família tem sido cada vez mais responsabilizada pelo cuidado em saúde, principalmente as mulheres alterando a dinâmica familiar que implica nas condições objetivas de vida

Additional file 1: of Castanea sativa Mill. bark extract exhibits chemopreventive properties triggering extrinsic apoptotic pathway in Jurkat cells

Morphological analysis of Jurkat cells in the absence and presence of CSM bark extract. Apoptosis-associated nuclear condensation and fragmentation were evaluated in untreated (A) and treated (B), (50 μg·mL−1 CSM bark extract for 72 h) Jurkat cells by fluorescence microscopy at 100× magnification. 3 × 105 Jurkat cells were loaded into cytospin chambers and centrifuged ad 450 rpm for 10 min. Cells were then fixed in formaldehyde 3.7%, permeabilised in 0.15% triton X-100 and nuclei were stained with 0.5 μM Hoechst 33,258 as reported by Henry et al. [51]. White arrows indicate condensed and/or fragmented nuclei as a marker of apoptosis. (TIFF 4797 kb

Effect of SFN 30 µM on RNA damage induced by doxorubicin, spermine, SNAP and H₂O₂ in HL-60 cells.

<p>RIN values were calculated after pre-treatment of cells with SFN for 24 h (6 h for H₂O₂). Data are means ± SEM of three independent experiments.</p

Effect of SFN on RNA integrity on Jurkat cell-free system.

<p>A representative electropherogram of RNA size distribution (A) and RIN values (B) after 3 h of treatment with SFN of a cell-free system. Data are means ± SEM of three independent experiments.</p

Effect of spermine plus SFN on RNA damage in Jurkat cells.

<p>RIN values calculated after pre-treatment (A) or co-treatment (B) of cells with SFN plus spermine and representative electropherograms of RNA size distribution after treatment with spermine plus SFN (C). Data are means ± SEM of three independent experiments.</p

Effect of SNAP plus SFN on RNA damage in Jurkat cells.

<p>RIN values calculated after pre-treatment (A) or co-treatment (B) of cells with SFN plus SNAP and representative electropherograms of RNA size distribution after treatment with SNAP plus SFN (C). Data are means ± SEM of three independent experiments.</p

Effect of SFN on RNA integrity on Jurkat cell system.

<p>A representative electropherogram of RNA size distribution (A) and RIN values (B) calculated after cell treatment with SFN for 24 h. Data are means ± SEM of three independent experiments.</p

Effect of SFN and spermine on glutathione levels in Jurkat cells.

<p>Effect of SFN, spermine and SFN plus spermine on glutathione level determined by microplate fluorescence reader. Cells were treated for 3 h (A) or 24 h (B) with SFN (10 µM), spermine (0.5 mM) or SFN (10 µM)+spermine (0.5 mM). Results are means ± SEM of four independent experiments with triplicate dishes.</p

Effect of H₂O₂ plus SFN on RNA damage in Jurkat cells.

<p>RIN values calculated after pre-treatment (A) or co-treatment (B) of cells with SFN plus H₂O₂ and representative electropherograms of RNA size distribution after treatment with H₂O₂ plus SFN (C). Data are means ± SEM of three independent experiments.</p

Induction of apoptosis by SFN plus spermine or doxorubicin in Jurkat cells.

<p>Fraction of apoptotic cells following pre- or co-treatment conducted with SFN (10 µM) and spermine (0.5 mM) or doxorubicin (0.01 mM), respectively. Results are means ± SEM of four independent experiments with triplicate dishes.</p