1 research outputs found
Catalyst-Free Aqueous Hyperpolarized [1-<sup>13</sup>C]Pyruvate Obtained by Re-Dissolution Signal Amplification by Reversible Exchange
Despite
great successes in oncology, patient outcomes are often
still discouraging, and hence the diagnostic imaging paradigm is increasingly
shifting toward functional imaging of the pathology to better understand
individual disease biology and to personalize therapies. The dissolution
Dynamic Nuclear Polarization (d-DNP) hyperpolarization method has
enabled unprecedented real-time MRI sensing of metabolism and tissue
pH using hyperpolarized [1-13C]pyruvate as a biosensor
with great potential for diagnosis and monitoring of cancer patients.
However, current d-DNP is expensive and suffers from long hyperpolarization
times, posing a substantial translational roadblock. Here, we report
the development of Re-Dissolution Signal Amplification By Reversible
Exchange (Re-D SABRE), which relies on fast and low-cost hyperpolarization
of [1-13C]pyruvate by chemical exchange with parahydrogen
at microtesla magnetic fields. [1-13C]pyruvate is precipitated
from catalyst-containing methanol using ethyl acetate and rapidly
reconstituted in aqueous media. 13C polarization of 9 ±
1% is demonstrated after redissolution in water with residual iridium
mass fraction of 8.5 ± 1.5 ppm; further improvement is anticipated
via process automation. Re-D SABRE makes hyperpolarized [1-13C]pyruvate biosensor available at a fraction of the cost (<$10,000)
and production time (≈1 min) of currently used techniques and
makes aqueous hyperpolarized [1-13C]pyruvate “ready”
for in vivo applications