Additional file 1: of DNMT1 regulates expression of MHC class I in post-mitotic neurons

Figure S1. Transfection efficiency of Cy3-labelled siRNA in CGNs. CGNs were transfected with Cy3-labelled non-targeting siRNA as described in Materials and Methods. After 72 h incubation the cells were fixated and mounted using mounting media containing DAPI. Epifluorescence images were obtained for DAPI, Cy3-siRNA and overlay. Arrows with thick arrowhead, transfected cell; thin arrowhead, non-transfected cell. Figure S2. Generation of a functional positive control of DNMT1 knockdown. (A) Gene expression of NNAT, CD24A, ICAM1, RUNX1, and S100A10 [46] in CGNs upon knockdown of DNMT1 relative to untreated cells. Bonferroni-corrected one-sample t-test: * p1.5 for NNAT and S100A10 relative to treatment with non-targeting siRNA. (C) Positive control values for three successful and four unsuccessful experiments (mean ± SD). Table S1. siRNAs from DharmaconTM used in the study. Table S2. Taqman probes used in the study. (DOCX 930 kb