1 research outputs found
The Combined Effect of Encapsulating Curcumin and C6 Ceramide in Liposomal Nanoparticles against Osteosarcoma
This
study examines the antitumor potential of curcumin and C6
ceramide (C6) against osteosarcoma (OS) cell lines when both are encapsulated
in the bilayer of liposomal nanoparticles. Three liposomal formulations
were prepared: curcumin liposomes, C6 liposomes and C6-curcumin liposomes.
Curcumin in combination with C6 showed 1.5 times enhanced cytotoxic
effect in the case of MG-63 and KHOS OS cell lines, in comparison
with curcumin liposomes alone. Importantly, C6-curcumin liposomes
were found to be less toxic on untransformed primary human cells (human
mesenchymal stem cells) in comparison to OS cell lines. In addition,
cell cycle assays on a KHOS cell line after treatment revealed that
curcumin only liposomes induced G<sub>2</sub>/M arrest by upregulation
of cyclin B1, while C6 only liposomes induced G<sub>1</sub> arrest
by downregulation of cyclin D1. C6-curcumin liposomes induced G<sub>2</sub>/M arrest and showed a combined effect in the expression levels
of cyclin D1 and cyclin B1. The efficiency of the preparations was
tested <i>in vivo</i> using a human osteosarcoma xenograft
assay. Using pegylated liposomes to increase the plasma half-life
and tagging with folate (FA) for targeted delivery <i>in vivo</i>, a significant reduction in tumor size was observed with C6-curcumin-FA
liposomes. The encapsulation of two water insoluble drugs, curcumin
and C6, in the lipid bilayer of liposomes enhances the cytotoxic effect
and validates the potential of combined drug therapy