Histology and histomorphometry ISA file (Metadata)

<p>The file contains (i) general information on the experiment and the protocols related to the dissection and histology and histomorphometry evaluations on the “i_Organism” tab, (ii) information on each animal in the “s_Organism” tab, (iii) information on the samples that were measured in the “s_DP_Histo” tab, (iv) the links between the metadata and the histopathology measurements given in the Data records 15 in the "a_DP_Histopathology” tab, (v) the links between<br>the metadata and the histomorphometry measurements given in the Data records 15<br>in the “a_DP_Histomorphometry” tab</p

Hematology ISA file (Metadata)

<p>The file contains (i) general information on the experiment and the protocols related to the dissection and hematology measurement on the “i_Organism” tab, (ii) information on each animal in the “s_Organism” tab, (iii) information on the samples that were measured in the “s_DP_Hematology” tab, and (iv) information on the measurements performed for hematology in the “a_DP_Hematology” tab.</p

BALF FACS analyses reports

<p>pdf reports of all BALF FACS analyses</p

Lung Histopathology and Histomorphometry Raw Data

<p>The file contains histopathology scores and histomorphometry measurements . Briefly, it has the exposure group information on the first row, the time point information on the second row, and the animal number information in the third row. Then, the first column has the name of the finding evaluated, or the parameter in the histomorphometry measured and the second column the level at which this was done.</p

Smoke chemistry

<p>The file contains the concentrations of 58 harmful and potentially harmful constituents measured in aerosols from 3R4F and the pMRTP.</p

Proteomics 2D gel ISA file (Metadata)

<p>The file contains (i) general information on the experiment and the protocols related to 2d gel proteomics on the “i_Organism” tab, (ii) information on each animal in the “s_Organism” tab, (iii) information on the samples that were measured in the “s_DP_Prot” tab, (iv) the links between the metadata and the image files given in the Data records 17 in the “a_DP_Prot” tab, (v) information on the data that was normalized in the “s_PR_Prot” tab, (vi) the links between the metadata and the normalized matrix given in the file 2D-PAGE data - normalized matrix</p

2D-PAGE data - normalized matrix

<p>Normalilzed matrix of protein measurements in lung by 2d-gel analysis.</p

Proteomics reverse phase protein array data

<p>For the correction of anti-species secondary antibody staining, arrays were assayed in the absence of primary antibodies. For the measurement of spotted protein, one blank chip (i.e., without antibody incubation) was stained with SYPROÒRuby. Scanned images were analyzed using ZeptoVIEW 3.1 software (Bayer Technology Services GmbH). Normalized fluorescence intensities (NFIs) for each sample and protein target were calculated as reference fluorescence intensities of primary antibody stained arrays (RFIprimary) corrected for secondary antibody staining (RFIsecondary), as well as relative spotted protein concentration (RFIprotein), determined by (RFIprimary − RFIsecondary) / RFIprotein, using ZeptoVIEW 3.1 software. NFI values were used for subsequent analysis.</p> <p>A series of bovine serum albumin references were spotted on each array. Each sample underwent serial dilution (100%, 75%, 50%, and 25%) to assess linearity. Prior to RPA analysis, all antibodies were validated by western blotting to assess their specificity.</p

Proteomics 2D gel images

<p>images of the 2d gels for lung proteomics analysis</p

Toxicity of aerosols of nicotine and pyruvic acid (separate and combined) in Sprague–Dawley rats in a 28-day OECD 412 inhalation study and assessment of systems toxicology

<div><p></p><p>Toxicity of nebulized nicotine (Nic) and nicotine/pyruvic acid mixtures (Nic/Pyr) was characterized in a 28-day Organization for Economic Co-operation and Development 412 inhalation study with additional transcriptomic and lipidomic analyses. Sprague–Dawley rats were nose-only exposed, 6 h/day, 5 days/week to filtered air, saline, nicotine (50 µg/l), sodium pyruvate (NaPyr, 33.9 µg/l) or equimolar Nic/Pyr mixtures (18, 25 and 50 µg nicotine/l). Saline and NaPyr caused no health effects, but rats exposed to nicotine-containing aerosols had decreased body weight gains and concentration-dependent increases in liver weight. Blood neutrophil counts were increased and lymphocyte counts decreased in rats exposed to nicotine; activities of alkaline phosphatase and alanine aminotransferase were increased, and levels of cholesterol and glucose decreased. The only histopathologic finding in non-respiratory tract organs was increased liver vacuolation and glycogen content. Respiratory tract findings upon nicotine exposure (but also some phosphate-buffered saline aerosol effects) were observed only in the larynx and were limited to adaptive changes. Gene expression changes in the lung and liver were very weak. Nic and Nic/Pyr caused few significant changes (including Cyp1a1 gene upregulation). Changes were predominantly related to energy metabolism and fatty acid metabolism but did not indicate an obvious toxicity-related response. Nicotine exposure lowered plasma lipids, including cholesteryl ester (CE) and free cholesterol and, in the liver, phospholipids and sphingolipids. Nic, NaPyr and Nic/Pyr decreased hepatic triacylglycerol and CE. In the lung, Nic and Nic/Pyr increased CE levels. These data suggest that only minor biologic effects related to inhalation of Nic or Nic/Pyr aerosols were observed in this 28-day study.</p></div