Supplemental material from Characterization in humans of <i>in vitro</i> leucocyte maximal telomerase activity capacity and association with stress

Protocol for isolation, stimulation and lysis of PBM

Barplot comparing metabolic ratios as indicators of β-oxidation (right) and carnitine palmitoyl transferase-1 activity (left) across trimesters among pregnant women.

<p>Median (+ Interquartile range/2) was plotted * p-value < 0.00017, p-value was calculated by Mann-Whitney U Test between trimesters.</p

Barplot comparing plasma non-esterified fatty acid concentrations across trimesters among pregnant women.

<p>Median (+ Interquartile range/2) was plotted * p-value < 0.00017, p-value was calculated by Mann-Whitney U Test between trimesters.</p

Barplot comparing plasma concentrations of tricarboxylic acid cycle intermediates and keto acids across trimesters among pregnant women.

<p>Median (+ Interquartile range/2) was plotted * p-value < 0.00017, p-value was calculated by Mann-Whitney U Test between trimesters.</p

Maternal and child characteristics.

<p>Maternal and child characteristics.</p

Maternal Pre-pregnancy BMI and Child ADHD Symptoms and Executive Function.

<p>Children born to mothers who were obese preconceptionally showed a) higher ADHD symptoms than children born to normal weight or overweight mothers and b) impaired executive function compared to children born to normal weight or overweight mothers. *p<0.05; **p<0.01.</p

Mediation Analysis for Effects of Maternal Pre-pregnancy BMI on Child ADHD Symptoms via Child Executive Function.

<p>The association between pre-pregnancy BMI and severity of child ADHD symptoms was significantly reduced when the measure of performance efficiency in the executive function task was included in the model; Sobel Test: t = 2.39 (±0.002, SEM), p = 0.02.</p

Estimates of effects of mother’s risk factors and mother’s genotype (CRHBP SNP rs1053989) on infant birth weight in grams.

*<p>Prob≤0.10.</p>**<p>Prob≤0.05.</p>***<p>Prob≤0.01.</p

Subject characteristics.

*<p>α = 0.05.</p>**<p>α = 0.01.</p>***<p>α = 0.001.</p

Significant SNP genotype effects on birth weight beyond risk factors.

1<p>Model A: Birth weight = Gestation Age + Child Sex + SNP.</p>2<p>Model B: Birth weight = Gestation Age + Child Sex + Pre-pregnancy BMI + Parity + Smoking + SNP.</p>3<p>% Genetic Variance from combined genotypes.</p>*<p>Prob≤0.10.</p>**<p>Prob≤0.05.</p>***<p>Prob≤0.01.</p