9 research outputs found
Effect of Rabbit Epididymal Antimicrobial Peptide, REHbβP, on LPS-Induced Proinflammatory Cytokine Responses in Human Vaginal Cells In Vitro
Antimicrobial peptides (AMP's) protect epithelial surfaces including epididymis against pathogens and play a key role in orchestrating various defensive responses. Recently, we have identified one such AMP, rabbit epididymal hemoglobin-β subuit (REHbβP) from the epididymal fluid of rabbit, Oryctologus cuniculus. The demonstration of a protective role of REHbβP in epididymal epithelial cells (EPEC's) led us to investigate: (1) the identification of LPS interactive domain in REHbβP, and (2) whether the REHbβP of rabbit origin mediates vaginal cellular immune responses of another species (human). HeLa-S3, human vaginal epithelial cells (hVECs) were exposed to LPS or the LPS-stimulated cells treated with REHbβP or neutral peptide, nREHbβP. Effect of LPS and cytokines (IL-6 and IL-1α) and chemokines (IL-8, MCP-1) levels was determined in the culture supernatants. In response to the LPS, hVECs synthesized these mediators and the levels were significantly higher than controls. This enhancing effect was ameliorated when the LPS-induced hVECs were treated with REHbβP. Similar results were obtained on NF-κB protein and hBD-1 mRNA expression. Confocal microscopy studies revealed that REHbβP attenuated the LPS-induced internalization of E. coli by macrophages. The chemotaxis studies performed using Boyden chamber Transwell assay, which showed elevated migration of U937 cells when the supernatants of LPS-induced hVECs were used, and the effect was inhibited by REHbβP. REHbβP was found to be localized on the acrosome of rabbit spermatozoa, suggesting its role in sperm protection beside sperm function. In conclusion, REHbβP may have the potential to develop as a therapeutic agent for reproductive tract infections (RTI's)
Effect of different shading intensities on growth and yield of cherry tomato
Cherry tomato (Solanum lycopersicon var cerasiforme) is small size fruits, with a bright red colour resembling to cherry and becoming popular in the retail chains which are marketed at a premium price. The field experiment was conducted to study the effect of different (35, 50 and 75 per cent) shading intensities on growth and yield (qha-1 ) of cherry tomato. Significantly maximum yield was recorded in 35 per cent shading intensity and genotype Kalash Seeds Product (KSP)-113 (579.44 and 503.88 q ha-1 , respectively). Among the different shading intensities and genotypes, maximum plant height was observed in 75 per cent shading intensity and genotype KSP -113 at 30 days interval (74.70 and 60.95 cm, respectively). The minimum days to 50% flowering of cherry tomato were observed in cherry tomatoes grown under 35 per cent shading intensity (45.00 days) as compared to other shading intensities while minimum days to 50% flowering were observed in genotype KSP-113 (44.00 days). The maximum length of the cluster (9.58 cm), the weight of cluster (27.67 g), number of fruits per cluster (9.42) and number of pickings (11.67) were observed in 35 per cent shading intensities and in genotype KSP-113. The cultivation of KSP-113 genotype under 35 per cent shading intensity was found to be most sustainable for improving growth and yield of cherry tomato during the summer season
Effect of different shading intensities on growth and yield of cherry tomato
Cherry tomato (Solanum lycopersicon var cerasiforme) is small size fruits, with a bright red colour resembling to cherry and becoming popular in the retail chains which are marketed at a premium price. The field experiment was conducted to study the effect of different (35, 50 and 75 per cent) shading intensities on growth and yield (qha-1 ) of cherry tomato. Significantly maximum yield was recorded in 35 per cent shading intensity and genotype Kalash Seeds Product (KSP)-113 (579.44 and 503.88 q ha-1 , respectively). Among the different shading intensities and genotypes, maximum plant height was observed in 75 per cent shading intensity and genotype KSP -113 at 30 days interval (74.70 and 60.95 cm, respectively). The minimum days to 50% flowering of cherry tomato were observed in cherry tomatoes grown under 35 per cent shading intensity (45.00 days) as compared to other shading intensities while minimum days to 50% flowering were observed in genotype KSP-113 (44.00 days). The maximum length of the cluster (9.58 cm), the weight of cluster (27.67 g), number of fruits per cluster (9.42) and number of pickings (11.67) were observed in 35 per cent shading intensities and in genotype KSP-113. The cultivation of KSP-113 genotype under 35 per cent shading intensity was found to be most sustainable for improving growth and yield of cherry tomato during the summer season