28 research outputs found
Mycotoxin production from fungi isolated from grapes
Aims: In order to assess the potential for producing mycotoxins, fungi were isolated from
wine producing grapes.
Methods and Results: The isolates were identified and Penicillium expansum, the most well
recognized mycotoxin producer, was analysed for mycotoxin production by TLC. Many of the
strains produced patulin and/or citrinin, often depending on whether they were grown on a
grape or yeast extract sucrose media.
Conclusions: Citrinin was produced by all strains grown in the yeast extract sucrose medium,
but only one strain (from 51) was able to produce this compound in grape juice medium.
Patulin was produced in the yeast extract medium by 20 strains and in grape juice medium by
33 strains.
Significance and Impact of the Study: The presence of mycotoxins in wine producing
grapes is discussed. Grapes contamination with patulin seems not to contribute to wine
contamination, and no ochratoxin producing fungi was identified.The British Council, Conselho de Reitores das Universidades Portuguesas (CRUP) - Windsor Programme - grant no 29/00
Wetting films on chemically heterogeneous substrates
Based on a microscopic density functional theory we investigate the
morphology of thin liquidlike wetting films adsorbed on substrates endowed with
well-defined chemical heterogeneities. As paradigmatic cases we focus on a
single chemical step and on a single stripe. In view of applications in
microfluidics the accuracy of guiding liquids by chemical microchannels is
discussed. Finally we give a general prescription of how to investigate
theoretically the wetting properties of substrates with arbitrary chemical
structures.Comment: 56 pages, RevTeX, 20 Figure
Challenges for standardization of Clostridium difficile typing methods
Typing of Clostridium difficile facilitates understanding of the epidemiology of the infection. Some evaluations have shown that certain strain types (for example, ribotype 027) are more virulent than others and are associated with worse clinical outcomes. Although restriction endonuclease analysis (REA) and pulsed-field gel electrophoresis have been widely used in the past, PCR ribotyping is the current method of choice for typing of C. difficile. However, global standardization of ribotyping results is urgently needed. Whole-genome sequencing of C. difficile has the potential to provide even greater epidemiologic information than ribotyping
Die Rolle der Bohrtechnik bei der Untertage-Umwandlung von Kohle
SIGLECopy held by FIZ Karlsruhe; available from UB/TIB Hannover / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekDEGerman