Biochemical and Pathomorphological Study of Potassium Dichromate-induced Nephrotoxicity in Wistar Rat

An experiment was conducted to study biochemical and pathomorphological alterations induced by potassium dichromate toxicity. Forty colony bred Albino Wistar strain rats of both sexes, divided uniformly into four equal groups Group A, Group B, Group C, and Group D. Each Group contains five male and five female. Group A rats received only deionised water and served as control. Group B (low dose), Group C (mid dose) and Group D (high dose) rats were given potassium dichromate orally by gavage for 28 days at the rate of 0.625 mg/kg body weight (b.wt.), 1.25 mg/kg b.wt. and 2.5 mg/kg b.wt. respectively. A dose dependant significant rise in plasma alanine aminotransferase (ALT), plasma aspartate aminotransferase (AST), plasma alkaline phosphatase (ALP), creatinine and blood urea nitrogen was observed in treatment group, whereas, a significant decrease in total protein and albumin was observed in treatment group. Histopathological sections of kidney, liver, lung and testes revealed varying degrees of congestion, haemorrhage, degeneration and necrosis in rats of different treatment groups. The present study indicates nephric and hepatic toxicity in albino wistar rats due to potassium dichromate toxicity

Učinak ricinoleične kiseline iz ricinusova ulja na proizvodna svojstva i hemato-biokemijski profil krava pasmine Kankrej u laktaciji

The aim of the present study was to evaluate the effects of dietary ricinoleic acid from castor oil on the milk yield, composition, fatty acid profile and haemato-biochemical profile in lactating Kankrej cows. Twenty lactating Kankrej cows were assigned to the following groups: control (CON), basal diet without any additive and treatment (RAS), basal diet with 2 g/animal/day of ricinoleic acid from castor oil. Dietary ricinoleic acid had no (P>0.05) effect on dry matter intake and feed efficiency. Milk yield (milk, 4% fat corrected milk and energy corrected milk) were not affected (P>0.05) by the feeding of ricinoleic acid. The milk composition showed no significant differences between the groups. A significantly (P0.05) in haemato-biochemical profile between the CON and RAS groups. It was concluded that supplementation of 2 g/animal/day ricinoleic acid from castor oil had no effect on milk yield, milk composition and haemato-biochemical profile. However, it increased the milk fat percentages of unsaturated fatty acids and polyunsaturated fatty acids, and decreased milk saturated fatty acids, without any adverse effect on the health status of the cows.Cilj istraživanja bio je procijeniti učinak ricinoleične kiseline iz ricinusova ulja na prinos mlijeka, kemijski sastav mlijeka, profil masnih kiselina u mlijeku te na hemato-biokemijski profil krava pasmine Kankrej u laktaciji. Ukupno 20 krava u laktaciji podijeljeno je u: kontrolnu skupinu (CON), skupinu koja je dobivala osnovnu hranu, bez dodataka i pokusnu skupinu (RAS), koja je dobivala osnovnu hranu i 2 g po životinji dnevno ricinoleične kiseline iz ricinusova ulja. Ricinoleična kiselina nije imala učinka (P>0,05) na unos suhe tvari i iskorištavanje hrane. Dodatak ricinoleične kiseline nije utjecao (P>0,05) na prinos mlijeka (mlijeko, obogaćeno mlijeko s 4 % masnoće i energijski obogaćeno mlijeko). Sastav mlijeka nije pokazao znakovite razlike među skupinama. Uočen je znakovito veći postotak C4:0, C6:0 i C8:0 u mlijeku u skupini RAS (P0,05) u hemato-biokemijskom profilu skupina CON i RAS. Zaključeno je da dodatak 2 g ricinoleične kiseline po životinji dnevno ne utječe na prinos i sastav mlijeka te hemato- biokemijski profil, međutim povećava postotak mliječne masti, nezasićenih masnih kiselina i višestruko nezasićenih masnih kiselina, a smanjuje postotak zasićenih masnih kiselina bez posljedica za zdravlje krava