L'apporto della tecnologia di protoplasti per il miglioramento delle specie legnose

93 ref., Polycopie des cours a l'Universite de Bologne, Italie*INRA, Centre d'Angers Diffusion du document : INRA, Centre d'AngersInternational audienc

Somatic hybridization between Pyrus x Prunus species

Somatic hybridization between[i] Pyrus x Prunus[/i] specie

The time-course evolution of viability and competence for proliferation of woody plant protoplasts following cold-storage

International audienc

Protoplast technology for the breeding of top-fruit trees (Prunus, Pyrus, Malus, Rubus) and woody ornamentals

International audienc

Assessments of graft-compatibility of somatic hybrids, Pyrus communis var., pyraster L. (+) Prunus avium x Pseudocerasus, and species of the subfamilies Pomoideae and Prunoideae, Rosaceae

Assessments of graft-compatibility of somatic hybrids, [i]Pyrus communis[/i] var., pyraster L. (+) [i]Prunus avium[/i] x [i]Pseudocerasus[/i], and species of the subfamilies Pomoideae and Prunoideae, Rosaceae. VII. International Congress on Plant Tissue and Cell Cultur

Regeneration de plantes a partir de protoplastes de pommier (Malus X domestica Borkh). Etude des exigences de culture pour differents genotypes apparentes de ploidie differente

*INRA, Station d'Amelioration des Especes Fruitieres et Ornementales, 42 rue Georges Morel, B.P. 57, 49071, Beaucouze Cedex Diffusion du document : INRA, Station d'Amelioration des Especes Fruitieres et Ornementales, 42 rue Georges Morel, B.P. 57, 49071, Beaucouze Cedex Diplôme : Dr. d'Universit

From stem protoplasts to complete plants of golden delicious apple (Malus Xdomestica Borich.)

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Protoplasts

8 ref., 2 tables, chap. 4International audienc

Flow cytometric analysis and molecular characterization of Agrobacterium tumefaciens-mediated transformants of Medicago truncatula

Sur la publication, l'auteur S. Djennane est mal orthographié (Djenanne).International audienceLeaf explants from leaflets collected from either in vivo grown or in vitro grown seedlings of Medicago truncatula genotype R108-1 were co-cultivated with bacterial cells of Agrobacterium tumefaciens strains EHA105 or C58pMP90. Each of these strains was carrying the pCambia 1390 plasmid harbouring a hygromycin resistance gene cassette. Explants were then incubated on a medium containing 10 mg/l hygromycin and 800 mg/l augmentin to suppress Agrobacterium growth, and subcultured 4-5 times every 2 weeks for the proliferation of calli. After 8-10 weeks, callusing explants were transferred to hormone-free medium with 10 mg/l hygromycin and 400 mg/l augmentin for shoot regeneration. After rooting, a total of about 300 putative transformants were grown into plantlets, transferred to soil, acclimatized, and then moved to the greenhouse. Of these, a total of 43 independent PCR positive primary transformants and their T1 and T2 progeny were subjected to flow cytometric analysis, to assessing their trueness-to-type, as well as to southern blot analysis

Flow cytometry measurements contribute to<em> Pisum taxonomy</em>

International audiencePea (Pisum sativum L.) has been widely used in early hybridization studies, as model for experimental morphology and physiology, and was Mendel’s model species to untangle the laws of inheritance, which puts it at the foundation of modern genetics. Its large genome size (4775 Mbp as assessed by Feulgen method in 1976) slowed down progress in pea genomics compared with other plant species, but the recent availability of genome sequences of various legume species now permits genome wide comparison and allows to identify genes underlying agronomically important traits by combining candidate gene and synteny approaches. The efficient use of existing genomic resources is a key to success in these goals and several types of molecular marker sets as well as both transcriptome and proteome datasets exist. Despite this impressive background, and the fact that P. sativum is one of the most frequently used internal standards for flow cytometry studies with other species, there is still a need to further clarify the taxonomy within species of the genus Pisum. Thus, we have analysed by flow cytometry 42 accessions from a range of geographic origins and belonging to two wild species: P. sativum subsp. elatius (e.g. including former P. elatius and P. humile/syriacum), P. fulvum and cultivated: P. abyssinicum, P. sativum as well as some primitive P. sativum cultigens (such as subsp. transcaucassicum, asiaticum), where some of them had been identified differently or tentatively in the past based on botanical characteristics. In these studies, all materials were analysed simultaneously with Medicago truncatula as the internal standard, and with various fluorochromes (DAPI, Propidium Iodide, Chromomycine A3) to assess the relative nuclear DNA content, genome size and AT/GC ratio. For some of the species studied this is the first report on these traits