Optimització i validació de metodologies analítiques per a la determinació de contaminants orgànics prioritaris en mostres vegetals

Treballs Finals de Grau de Química, Facultat de Química, Universitat de Barcelona, Any: 2018, Tutors: Xavier Cetó Alseda, Josep Maria Bayona TermensPolycyclic aromatic hydrocarbons (PAHs) are a large class of organic compounds widespread in the environment and are generated mainly by incomplete combustion of fossil fuels or by pyrolysis in industrial processes. According to IARC, some of these compounds have been catalogued as carcinogenic (Group 1), probably carcinogenic (Group 2A), possibly carcinogenic (Group 2B) and not classifiable as to carcinogenicity (Group 3) to humans, who are exposed to these compounds by inhalation of contaminated air or via dietary intake. Nowadays, there is no analytical methodology that guarantees the extraction and the detection of PAHs at a trace level in horticultural crops. For this reason, this project attempts the optimization of an analytical procedure in different vegetable matrices (lettuce, tomatoes, cauliflowers) using the ultrasound assisted extraction technique, followed by a clean-up step employing adsorption chromatography with alumina as a stationary phase and its detection with gas chromatography coupled to a tandem mass spectrometry (GC – MS/MS). With this analytical procedure developed, the limits of detection (LODs) were calculated for each vegetable as 432 ng/g - 16000 ng/g (lettuce in fresh weight), 439 ng/g - 16257 ng/g (tomato in fresh weight) and 456 ng/g - 16257 ng/g (cauliflower in fresh weight) but the recoveries and precision were poor for all the matrices evaluated (0.8% - 37.3% and 83.6%, respectively) stressing the complexit

Effects of tetracycline, sulfonamide, fluoroquinolone, and lincosamide load in pig slurry on lettuce: Agricultural and human health implications

The application of pig slurry as fertilizer in agriculture provides nutrients, but it can also contain veterinary medicines, including antibiotic residues (ABs), which can have an ecotoxicological impact on agroecosystems. Furthermore, uptake, translocation, and accumulation of ABs in crops can mobilize them throughout the food chain. This greenhouse study aims to assess AB uptake from soil fertilized with pig slurry and its phenotypical effects on Lactuca sativa LThe authors gratefully acknowledge the financial support of the Spanish Ministry of Science and Innovation through project AGL 2017- 89518-R. IDAEA-CSIC is a Severo Ochoa Centre of Excellence (Spanish Ministry of Science and Innovation, Project CEX 2018-000794-S). Monica Escola Casas wishes to thank the Beatriu de Pinos 2018 grant program (MSCA grant agreement number 801370) for the funding. The authors likewise thank Miquel Massip, Daniel Fenero, and Nerea Gran- ados for their technical assistance in the greenhouse facilityPostprint (published version