Cluster plot obtained after the HRM analysis using the VNTR-4bis and VNTR-Lb5.

<p>Cluster plot obtained after the HRM analysis using the VNTR-4bis and VNTR-Lb5.</p

The materials and methods implemented in this study for the genotyping of <i>Leptospira</i> at the subspecies level, and practical applications.

<p>The materials and methods implemented in this study for the genotyping of <i>Leptospira</i> at the subspecies level, and practical applications.</p

Melting curve analysis of pathogenic <i>Leptospira</i> strains after real-time PCR amplification using the G1/G2 and LFb1F/R primer sets.

<p>Melting curve analysis of pathogenic <i>Leptospira</i> strains after real-time PCR amplification using the G1/G2 and LFb1F/R primer sets.</p

Determination of the <i>Leptospira</i> species and genotypes with reference strains.

<p>Determination of the <i>Leptospira</i> species and genotypes with reference strains.</p

Determination of species and genotypes by real-time PCR with different sets of primers.

<p>*Sixteen reference strains were used to develop the typing method.</p><p>Determination of species and genotypes by real-time PCR with different sets of primers.</p

The materials and methods implemented in this study for the genotyping of <i>Leptospira</i> at the species level, and practical applications.

<p>The materials and methods implemented in this study for the genotyping of <i>Leptospira</i> at the species level, and practical applications.</p

Clusters of species and DNA extracted from sera with the VNTR 4bis and Lb5 primers shown in Fig 5.

<p>Clusters of species and DNA extracted from sera with the VNTR 4bis and Lb5 primers shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0127430#pone.0127430.g005" target="_blank">Fig 5</a>.</p

Number of included and confirmed patients, according to gender and age, gender odds ratio and confidence intervals, Guadeloupe and Martinique, 2011.

<p>Number of included and confirmed patients, according to gender and age, gender odds ratio and confidence intervals, Guadeloupe and Martinique, 2011.</p

Epidemiological and statistical approach used to estimate global leptospirosis incidence, French West Indies, 2011.

<p>Epidemiological and statistical approach used to estimate global leptospirosis incidence, French West Indies, 2011.</p

Number of included patients and results of leptospirosis diagnosis in Guadeloupe and Martinique, 2011.

<p>Number of included patients and results of leptospirosis diagnosis in Guadeloupe and Martinique, 2011.</p