Histological score for mice four days after intra-articular galectin-3 (gal-3) injection

<p><b>Copyright information:</b></p><p>Taken from "Extracellular localization of galectin-3 has a deleterious role in joint tissues"</p><p>http://arthritis-research.com/content/9/1/R20</p><p>Arthritis Research & Therapy 2007;9(1):R20-R20.</p><p>Published online 27 Feb 2007</p><p>PMCID:PMC1860078.</p><p></p> Total score, cartilage score and bone histomorphometric score. Data are expressed as median and (range) and are presented in box plot, where the boxes represent the 1st and 3rd quartiles, the line within the box represents the median, and the lines outside the box represent the spread of the values. Control (Ctl): mice injected with PBS. versus control group; = four animals per group

Effects of exogenous galectin-3 (gal-3) on markers of subchondral bone osteoblasts

<p><b>Copyright information:</b></p><p>Taken from "Extracellular localization of galectin-3 has a deleterious role in joint tissues"</p><p>http://arthritis-research.com/content/9/1/R20</p><p>Arthritis Research & Therapy 2007;9(1):R20-R20.</p><p>Published online 27 Feb 2007</p><p>PMCID:PMC1860078.</p><p></p> Osteoblasts were treated with 1 or 10 μg/ml of recombinant human gal-3 in the presence or absence of vitamin D. Both alkaline phosphatase and osteocalcin expression were analyzed by real time RT-PCR. Insert illustrates the protein level of osteocalcin. = 4

Representative histological sections of specimens from mice stained with safranin O or toluidine blue (magnification ×100)

<p><b>Copyright information:</b></p><p>Taken from "Extracellular localization of galectin-3 has a deleterious role in joint tissues"</p><p>http://arthritis-research.com/content/9/1/R20</p><p>Arthritis Research & Therapy 2007;9(1):R20-R20.</p><p>Published online 27 Feb 2007</p><p>PMCID:PMC1860078.</p><p></p> Control group (a,e). Mice were injected with 0.1 μg (b,f), 1 μg (c,g), or 10 μg (d,h) galectin-3

Effects of exogenous galectin-3 (gal-3) on type I collagen expression in osteoblasts

<p><b>Copyright information:</b></p><p>Taken from "Extracellular localization of galectin-3 has a deleterious role in joint tissues"</p><p>http://arthritis-research.com/content/9/1/R20</p><p>Arthritis Research & Therapy 2007;9(1):R20-R20.</p><p>Published online 27 Feb 2007</p><p>PMCID:PMC1860078.</p><p></p> Osteoblasts were treated with 1 or 10 μg/ml of recombinant human gal-3 in the presence or not of vitamin D. Collagen type I α1 chain expression was analyzed by real time RT-PCR. *versus control (Ctl; without vitamin D3 or gal-3); **versus 1 μg gal-3 alone; = 4

Signalling pathways of inhibition by galectin-3 (gal-3) of vitamin D-stimulated osteocalcin production

<p><b>Copyright information:</b></p><p>Taken from "Extracellular localization of galectin-3 has a deleterious role in joint tissues"</p><p>http://arthritis-research.com/content/9/1/R20</p><p>Arthritis Research & Therapy 2007;9(1):R20-R20.</p><p>Published online 27 Feb 2007</p><p>PMCID:PMC1860078.</p><p></p> Osteoblasts were treated with 5 μg/ml of recombinant human gal-3 in the presence of vitamin Dand osteocalcin was determined. Inhibitor concentrations were: KT5720, 2 μM; KT5823, 2 μM; Genistein (Genist.), 1 μM; Taxifollin, 1 μM; wortmannin (Wortma.), 250 nM; PD98059, 10 μM; and SB202190, 2 μM. *versus the autologous control; = 5