Dynamical separation of spherical bodies in supersonic flow

An experimental and computational investigation of the unsteady separation behaviour of two spheres in Mach-4 flow is carried out. The spherical bodies, initially contiguous, are released with negligible relative velocity and thereafter fly freely according to the aerodynamic forces experienced. In experiments performed in a supersonic Ludwieg tube, nylon spheres are initially suspended in the test section by weak threads which are detached by the arrival of the flow. The subsequent sphere motions and unsteady flow structures are recorded using high-speed (13 kHz) focused shadowgraphy. The qualitative separation behaviour and the final lateral velocity of the smaller sphere are found to vary strongly with both the radius ratio and the initial alignment angle of the two spheres. More disparate radii and initial configurations in which the smaller sphere centre lies downstream of the larger sphere centre each increases the tendency for the smaller sphere to be entrained within the flow region bounded by the bow shock of the larger body, rather than expelled from this region. At a critical angle for a given radius ratio (or a critical radius ratio for a given angle), transition from entrainment to expulsion occurs; at this critical value, the final lateral velocity is close to maximum due to the same ‘surfing’ effect noted by Laurence & Deiterding (J. Fluid Mech., vol. 676, 2011, pp. 396–431) at hypersonic Mach numbers. A visualization-based tracking algorithm is used to provide quantitative comparisons between the experiments and high-resolution inviscid numerical simulations, with generally favourable agreement

Fluid Mechanics of Everyday Objects

High speed Schlieren videos were produced highlighting the fluid mechanics found in everyday objects. This video (entry 102369) was submitted as part of the Gallery of Fluid Motion 2013, which is a showcase of fluid dynamics videos.Comment: Both a high-resolution version and a low-resolution version of the submitted video are available for downloa

Differential Interferometric Measurement of Instability at Two Points in a Hypervelocity Boundary Layer

The focused laser differential interferometer (FLDI) was used to investigate disturbances in a hypervelocity boundary layer on a sharp five degree half-angle cone. The T5 hypervelocity free-piston driven reflected-shock tunnel was used as the test facility; in such a facility the study of thermo-chemical/fluid-dynamic energy exchange is emphasized. Two sensitive FLDI probe volumes were aligned along a generator of the cone that recorded time-traces of density fluctuation at sufficient time resolution, spatial resolution, and signal to noise ratio, so that the boundary layer instability could be resolved. This arrangement of the FLDI allows for the interpretation of disturbances at two points and the correlation between them. The acoustic instability is detected with narrow-band peaks in the spectral response at a number of frequencies (500 kHz to 1.29 MHz). The data indicate that the instability driving the boundary layer to turbulence is acoustic in nature. Preliminary analysis indicates that there is not a significant difference between N2 and air acoustic boundary layer disturbance amplification factors for the representative cases presented. Computation of acoustic damping by thermo-chemical relaxation processes is presented for the same representative cases, and indicates that there is a negligible amount of absorption for both air and N_2 at the observed disturbance frequencies

The Dynamics of Internet Traffic: Self-Similarity, Self-Organization, and Complex Phenomena

The Internet is the most complex system ever created in human history. Therefore, its dynamics and traffic unsurprisingly take on a rich variety of complex dynamics, self-organization, and other phenomena that have been researched for years. This paper is a review of the complex dynamics of Internet traffic. Departing from normal treatises, we will take a view from both the network engineering and physics perspectives showing the strengths and weaknesses as well as insights of both. In addition, many less covered phenomena such as traffic oscillations, large-scale effects of worm traffic, and comparisons of the Internet and biological models will be covered.Comment: 63 pages, 7 figures, 7 tables, submitted to Advances in Complex System

Crossover recombination and synapsis are linked by adjacent regions within the N terminus of the Zip1 synaptonemal complex protein

Accurate chromosome segregation during meiosis relies on the prior establishment of at least one crossover recombination event between homologous chromosomes. Most meiotic recombination intermediates that give rise to interhomolog crossovers are embedded within a hallmark chromosomal structure called the synaptonemal complex (SC), but the mechanisms that coordinate the processes of SC assembly (synapsis) and crossover recombination remain poorly understood. Among known structural components of the budding yeast SC, the Zip1 protein is unique for its independent role in promoting crossover recombination; Zip1 is specifically required for the large subset of crossovers that also rely on the meiosis-specific MutSgamma complex. Here we report that adjacent regions within Zip1\u27s N terminus encompass its crossover and synapsis functions. We previously showed that deletion of Zip1 residues 21-163 abolishes tripartite SC assembly and prevents robust SUMOylation of the SC central element component, Ecm11, but allows excess MutSgamma crossover recombination. We find the reciprocal phenotype when Zip1 residues 2-9 or 10-14 are deleted; in these mutants SC assembles and Ecm11 is hyperSUMOylated, but MutSgamma crossovers are strongly diminished. Interestingly, Zip1 residues 2-9 or 2-14 are required for the normal localization of Zip3, a putative E3 SUMO ligase and pro-MutSgamma crossover factor, to Zip1 polycomplex structures and to recombination initiation sites. By contrast, deletion of Zip1 residues 15-20 does not detectably prevent Zip3\u27s localization at Zip1 polycomplex and supports some MutSgamma crossing over but prevents normal SC assembly and Ecm11 SUMOylation. Our results highlight distinct N terminal regions that are differentially critical for Zip1\u27s roles in crossing over and SC assembly; we speculate that the adjacency of these regions enables Zip1 to serve as a liaison, facilitating crosstalk between the two processes by bringing crossover recombination and synapsis factors within close proximity of one another

Differential Interferometric Measurement of Instability at Two Points in a Hypervelocity Boundary Layer

The focused laser differential interferometer (FLDI) was used to investigate disturbances in a hypervelocity boundary layer on a sharp five degree half-angle cone. The T5 hypervelocity free-piston driven reflected-shock tunnel was used as the test facility; in such a facility the study of thermo-chemical/fluid-dynamic energy exchange is emphasized. Two sensitive FLDI probe volumes were aligned along a generator of the cone that recorded time-traces of density fluctuation at sufficient time resolution, spatial resolution, and signal to noise ratio, so that the boundary layer instability could be resolved. This arrangement of the FLDI allows for the interpretation of disturbances at two points and the correlation between them. The acoustic instability is detected with narrow-band peaks in the spectral response at a number of frequencies (500 kHz to 1.29 MHz). The data indicate that the instability driving the boundary layer to turbulence is acoustic in nature. Preliminary analysis indicates that there is not a significant difference between N2 and air acoustic boundary layer disturbance amplification factors for the representative cases presented. Computation of acoustic damping by thermo-chemical relaxation processes is presented for the same representative cases, and indicates that there is a negligible amount of absorption for both air and N_2 at the observed disturbance frequencies

Correlations between tumor-infiltrating and circulating lymphocyte subpopulations in advanced renal cancer patients treated with nivolumab

Background: In clinical trials with immunotherapy, histological features such as tumor-infiltrating lymphocytes (TILs) are investigated as potential predictive biomarkers, with the limit of an outdated parameter for a typically dynamic element. Methods: This explorative study compared, in metastatic renal cell carcinoma (mRCC) patients, basal pathological data about TILs on diagnostic histological specimens with circulating lymphocyte subpopulations measured before and during therapy with nivolumab. Results: Of 11 mRCC patients, 5 had low presence of TILs (L-TILs), 3 moderate amount (M-TILs) and 3 high number (H-TILs). Overall, 8 patients had low intratumoral pathological CD4+/CD8+ ratio (LIPR) ≤1 and 3 cases high intratumoral pathological ratio (HIPR) ≥2. Of 8 patients with LIPR, only 2 matched with low circulating CD4+/CD8+ ratio (LCR) ≤1; 5 had high circulating ratio (HCR) ≥2. All 3 cases with HIPR (≥2) conversely had LCR (≤1). Circulating CD4+/CD8+ ratio remained unchanged during therapy (mean-0.12 in 8 weeks). The respective percentage values of CD4+ and CD8+ circulating T cells also remained stable (variation 0%); the absolute value of CD4+ was more likely to increase (mean +46.3/mm3); the level of CD8+ tended to slightly decrease (mean-6.5/mm3). No correlation of lymphocyte subpopulations with treatment outcome was found. Of note, we did not evidence correspondence between histopathological and circulating findings in terms of T-lymphocyte subpopulations, also suggesting the inconsistency of circulating data in terms of relative variations. Conclusions: Considering the likely high dynamism of TILs, rebiopsy before therapy might be proposed to assess the utility of TILs characterization for predictive purpose. (www.actabiomedica.it)

A Machine Learning Approach for Using the Postmortem Skin Microbiome to Estimate the Postmortem Interval

Research on the human microbiome, the microbiota that live in, on, and around the human person, has revolutionized our understanding of the complex interactions between microbial life and human health and disease. The microbiome may also provide a valuable tool in forensic death investigations by helping to reveal the postmortem interval (PMI) of a decedent that is discovered after an unknown amount of time since death. Current methods of estimating PMI for cadavers discovered in uncontrolled, unstudied environments have substantial limitations, some of which may be overcome through the use of microbial indicators. In this project, we sampled the microbiomes of decomposing human cadavers, focusing on the skin microbiota found in the nasal and ear canals. We then developed several models of statistical regression to establish an algorithm for predicting the PMI of microbial samples. We found that the complete data set, rather than a curated list of indicator species, was preferred for training the regressor. We further found that genus and family, rather than species, are the most informative taxonomic levels. Finally, we developed a k-nearest- neighbor regressor, tuned with the entire data set from all nasal and ear samples, that predicts the PMI of unknown samples with an average error of ±55 accumulated degree days (ADD). This study outlines a machine learning approach for the use of necrobiome data in the prediction of the PMI and thereby provides a successful proof-of- concept that skin microbiota is a promising tool in forensic death investigations

Patient-centred measurement in ophthalmology – a paradigm shift

Ophthalmologists and researchers in ophthalmology understand what a rapidly evolving field ophthalmology is, and that to conduct good research it is essential to use the latest and best methods. In outcomes research, one modern initiative has been to conduct holistic measurement of outcomes inclusive of the patient's point of view; patient-centred outcome. This, of course, means including a questionnaire. However, the irony of trying to improve outcomes research by being inclusive of many measures is that the researcher may not be expert in all measures used. Certainly, few people conducting outcomes research in ophthalmology would claim to be questionnaire experts. Most tend to be experts in their ophthalmic subspecialty and probably simply choose a popular questionnaire that appears to fit their needs and think little more about it. Perhaps, unlike our own field, we assume that the field of questionnaire research is relatively stable. This is far from the case. The measurement of patient-centred outcomes with questionnaires is a rapidly evolving field. Indeed, over the last few years a paradigm shift has occurred in patient-centred measurement