การพัฒนาผลิตภัณฑ์พุดดิ้งผักเพื่อผู้สูงอายุและผลของการเก็บรักษาผลิตภัณฑ์ต่อปริมาณโพลีฟีนอลทั้งหมดและฤทธิ์การต้านอนุมูลอิสระ(FORMULATION OF VEGETABLE PUDDING FOR ELDERLY AND THEIR STORAGE EFFECT ON TOTAL PHENOLICS AND ANTIOXIDANT ACTIVITIES)

พุดดิ้งเป็นอาหารที่อุดมไปด้วยสารอาหารต่างๆ ซึ่งมีส่วนประกอบหลักคือ นม น้ำตาล น้ำมัน และสารก่อเจล อีกทั้งพุดดิ้งมีเนื้อสัมผัสที่นุ่มและสามารถกลืนได้ง่าย ดังนั้นผลิตภัณฑ์พุดดิ้งจึงเหมาะสมสำหรับผู้สูงอายุที่มีปัญหาด้านการบดเคี้ยวเนื่องจากมีการสูญเสียฟัน ซึ่งงานวิจัยนี้มีวัตถุประสงค์เพื่อพัฒนาผลิตภัณฑ์พุดดิ้งที่มีสารอาหารครบถ้วน โดยมีการใช้ผักผง 3 ชนิด ได้แก่ มันเทศหวาน ข้าวโพดหวาน และฟักทอง ที่ระดับความเข้มข้นร้อยละ 8 เติมลงในพุดดิ้งสูตรต้นแบบ เพื่อศึกษาการยอมรับทางประสาทสัมผัส สี ปริมาณโพลีฟีนอลทั้งหมด ฤทธิ์การต้านอนุมูลอิสระ และผลของระยะเวลาในการเก็บรักษาผลิตภัณฑ์ จากการศึกษาพบว่าผลิตภัณฑ์พุดดิ้งผักจะมีสีเข้มกว่าพุดดิ้งสูตรต้นแบบ แต่ผลิตภัณฑ์พุดดิ้งทุกสูตรยังได้รับการยอมรับทางประสาทสัมผัส โดยมีความชอบโดยรวมที่ระดับความชอบปานกลาง สำหรับปริมาณโพลีฟีนอลทั้งหมดและฤทธิ์การต้านอนุมูลอิสระ พบว่าผลิตภัณฑ์พุดดิ้งผักทุกสูตรมีปริมาณโพลีฟีนอลทั้งหมดและฤทธิ์การต้านอนุมูลอิสระมากกว่าพุดดิ้งสูตรต้นแบบ โดยเฉพาะอย่างยิ่งในผลิตภัณฑ์พุดดิ้งข้าวโพดหวานและพุดดิ้งฟักทอง ส่วนผลิตภัณฑ์พุดดิ้งมันเทศหวานมีปริมาณโพลีฟีนอลทั้งหมดใกล้เคียงกับพุดดิ้งสูตรต้นแบบ แต่มีฤทธิ์การต้านอนุมูลอิสระมากกว่าพุดดิ้งสูตรต้นแบบ ผลิตภัณฑ์ทั้งหมดทำการเก็บรักษาที่อุณหภูมิ 35 องศาเซลเซียส เป็นเวลา 12 สัปดาห์ พบว่า ผลิตภัณฑ์มีสีคล้ำขึ้นตามระยะเวลาในการเก็บรักษา ส่วนปริมาณโพลีฟีนอลทั้งหมดของผลิตภัณฑ์ทุกสูตรมีการเพิ่มขึ้นสูงสุดที่สัปดาห์ที่ 6 และหลังจากนั้นมีการลดลงจนถึงสัปดาห์ที่ 12 สำหรับฤทธิ์การต้านอนุมูลอิสระพบว่ามีการเพิ่มขึ้นในระหว่างการเก็บรักษา และมีการลดลงที่สัปดาห์ที่ 12 ดังนั้นในการศึกษานี้จะเห็นได้ว่าการเติมผักผงและระยะเวลาในการเก็บรักษามีผลต่อปริมาณโพลีฟีนอลทั้งหมดและฤทธิ์การต้านอนุมูลอิสระ ผลิตภัณฑ์พุดดิ้งที่พัฒนาขึ้นนี้สามารถเป็นต้นแบบของผลิตภัณฑ์เสริมอาหารสำหรับผู้สูงอายุ ที่มีประโยชน์ต่อสุขภาพ นอกจากนี้ผลิตภัณฑ์นี้สามารถเป็นผลิตภัณฑ์สำหรับเด็กและผู้บริโภคที่ดูแลสุขภาพได้อีกด้วยคำสำคัญ: พุดดิ้งผัก  ปริมาณโพลีฟีนอลทั้งหมด  ฤทธิ์การต้านอนุมูลอิสระ  การเก็บรักษาPudding is a nutrient-rich dessert commonly served for elderly who is lack of chewing ability due to tooth loss because of its soft texture. Pudding is usually composed of milk, oil, sugar and hydrocolloids. The aim of this study was to formulate ready-to-eat pudding containing essential nutrients with varying types of vegetable powder added, including sweet potato (SP), sweet corn (SC), and pumpkin (PK) powder. The pudding was studied the sensory acceptability, color, total phenolic contents (TPC) and antioxidant activities (AA) measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and oxygen radical absorbance capacity (ORAC) assays. The effect of storage time on the color, TPC and AA of each developed formula was also investigated. The vegetable powder was added at 8% (w/w) separately into the control. All developed puddings were accepted by the panelists at like moderately of overall acceptability. Although, the color of vegetable puddings were darker than the control, the panelists still accepted. The TPC and AA of vegetable puddings were greater than that of the control, particularly SC and PK pudding. The TPC content of SP pudding was similar to that of the control, whilst the AA of it was higher than that of the control. The developed products stored at 35ºC for 12 weeks showed that the color was darker. TPC of all formulas were highest at week 6, and then declined until week 12. The AA of the vegetable pudding increase somewhere during storage time, then reduced at the week 12. This study demonstrated that vegetable powder affected TPC and AA. Furthermore, the storage time played an important role on TPC and AA. These products are prototype of supplement for elderly which have health benefits beyond basic nutrition. In addition, these products can be extended to children and health conscious consumers as well.Keywords: Vegetable Pudding, Total Phenolic Contents, Antioxidant Activities, Storag

Anti-Inflammatory Activity and Mechanism of Sweet Corn Extract on Il-1β-Induced Inflammation in a Human Retinal Pigment Epithelial Cell Line (ARPE-19)

Age-related macular degeneration (AMD) is an eye disease associated with aging. Development of AMD is related to degeneration and dysfunction of the retinal pigment epithelium (RPE) caused by low-grade chronic inflammation in aged RPE cells leading to visual loss and blindness. Sweet corn is a good source of lutein and zeaxanthin, which were reported to exert various biological activities, including anti-inflammatory activity. The present study aims to investigate the anti-inflammatory activity and mechanisms of SCE to inhibit the production of inflammatory biomarkers related to AMD development. Cells were pretreated with SCE for 1 h followed by stimulation with IL-1β for another 24 h. The results demonstrated that SCE attenuated IL-1β-induced production of IL-6, IL-8, and MCP-1 and the expression of ICAM-1 and iNOS in a dose-dependent manner. In addition, SCE suppressed the phosphorylation of ERK1/2, SAPK/JNK, p38, and NF-κB (p65) in IL-1β-stimulated ARPE-19 cells. These results proved that SCE protected ARPE-19 cells from IL-1β-induced inflammation by inhibiting inflammatory markers partly via suppressing the activation of MAPK and NF-κB signaling pathways. Overall, SCE is a potential agent for the prevention of AMD development, which should be further evaluated in animals

Anti-Inflammatory Activity and Mechanism of Sweet Corn Extract on Il-1β-Induced Inflammation in a Human Retinal Pigment Epithelial Cell Line (ARPE-19)

Age-related macular degeneration (AMD) is an eye disease associated with aging. Development of AMD is related to degeneration and dysfunction of the retinal pigment epithelium (RPE) caused by low-grade chronic inflammation in aged RPE cells leading to visual loss and blindness. Sweet corn is a good source of lutein and zeaxanthin, which were reported to exert various biological activities, including anti-inflammatory activity. The present study aims to investigate the anti-inflammatory activity and mechanisms of SCE to inhibit the production of inflammatory biomarkers related to AMD development. Cells were pretreated with SCE for 1 h followed by stimulation with IL-1β for another 24 h. The results demonstrated that SCE attenuated IL-1β-induced production of IL-6, IL-8, and MCP-1 and the expression of ICAM-1 and iNOS in a dose-dependent manner. In addition, SCE suppressed the phosphorylation of ERK1/2, SAPK/JNK, p38, and NF-κB (p65) in IL-1β-stimulated ARPE-19 cells. These results proved that SCE protected ARPE-19 cells from IL-1β-induced inflammation by inhibiting inflammatory markers partly via suppressing the activation of MAPK and NF-κB signaling pathways. Overall, SCE is a potential agent for the prevention of AMD development, which should be further evaluated in animals

Nutritional Security: Carbohydrate Profile and Folk Remedies of Rare Edible Mushrooms to Diversify Food and Diet: Thailand Case Study

The aim of this study was to explore the current knowledge and practice of agrobiodiversity to improve nutrition and health. The study focused on wild mushrooms commonly consumed in Northeastern Thailand in terms of ecology, cooking and preservation methods, and folk remedies. The monosaccharide, sugar alcohol, glucan, and carbohydrate polymer content and the glycemic index of these wild mushrooms were determined using the enzymatic method. The mushrooms collected belonged to three biological groups and were mostly saprotrophic and symbiotic. The most abundant mushrooms were Amanita, Boletus, and Russula, followed by Calostoma sp., Astraeus asiaticus C. Phosri, and Astraeus odoratus C. Phosri. Wild edible mushrooms can be used for food and medicinal purposes. Cooking methods utilized in the area consist of steaming, boiling, and grilling. Glucose was the major monosaccharide detected in all mushroom samples. Xylitol and inositol were found in all mushroom samples, while some contained mannitol and arabitol. Glucan was present in all mushroom samples, ranging from 8.03 to 31.1 mg/g DW. All mushrooms were classified as having a low glycemic index. These findings provide important information to potentially enhance and promote the utilization of wild mushrooms to improve the accessibility, availability, and sustainability of nutritious food

Phytochemicals and In Vitro Bioactivities of Aqueous Ethanolic Extracts from Common Vegetables in Thai Food

Non-communicable diseases (NCDs) are the leading global cause of death. The World Health Organization (WHO) has endorsed the consumption of fruits and vegetables because they are rich in phytochemicals that sustainably ameliorate the occurrence of NCDs. Thai food contains many spices and vegetables with recognized health benefits. Quality control of plant samples encountered a bottleneck in the field and comparative studies of plant control origins including species or cultivar identification, growing area and appropriate harvesting time are limited. To address this issue, all plant samples used in this study were cultivated and controlled by the Department of Agriculture, Ministry of Agriculture and Cooperatives, Thailand. The samples were phytochemically screened and determined their health-promoting bioactivities via antioxidant activities and inhibition of NCD-related enzymes including lipase (obesity), α-amylase and α-glucosidase (diabetes), angiotensin-converting enzyme (hypertension), as well as acetylcholinesterase, butyrylcholinesterase and β-secretase (Alzheimer’s disease). The non-enzymatic reaction toward glycation was also evaluated. The results showed that Senegalia pennata subsp. insuavis (Lace) Maslin, Seigler & Ebinger, Citrus hystrix DC. and Solanum melongena ‘Kermit’ extracts exhibited high antioxidant activities. Moreover, Citrus hystrix DC. extract was a potent inhibitor against lipase, angiotensin-converting enzyme and butyrylcholinesterase, while Coriandrum sativum L. and Psophocarpus tetragonolobus (L.) DC. were potent anti-diabetic agents and Senegalia pennata subsp. insuavis (Lace) Maslin, and Seigler & Ebinger was a potent anti-glycation agent. Our data provide a comparative analysis of ten vegetables to encourage healthy food consumption and development to control NCDs in Thailand in the future

Astaxanthin-Loaded Pickering Emulsions Stabilized by Nanofibrillated Cellulose: Impact on Emulsion Characteristics, Digestion Behavior, and Bioaccessibility

Astaxanthin (AX) is one of the major bioactives that has been found to have strong antioxidant properties. However, AX tends to degrade due to its highly unsaturated structure. To overcome this problem, a Pickering O/W emulsion using nanofibrillated cellulose (NFC) as an emulsifier was investigated. NFC was used because it is renewable, biodegradable, and nontoxic. The 10 wt% O/W emulsions with 0.05 wt% AX were prepared with different concentrations of NFC (0.3–0.7 wt%). After 30 days of storage, droplet size, ζ-potential values, viscosity, encapsulation efficiency (EE), and color were determined. The results show that more stable emulsions are formed with increasing NFC concentrations, which can be attributed to the formulation of the NFC network in the aqueous phase. Notably, the stability of the 0.7 wt% NFC-stabilized emulsion was high, indicating that NFC can improve the emulsion’s stability. Moreover, it was found that fat digestibility and AX bioaccessibility decreased with increasing NFC concentrations, which was due to the limitation of lipase accessibility. In contrast, the stability of AX increased with increasing NFC concentrations, which was due to the formation of an NFC layer that acted as a barrier and prevented the degradation of AX during in vitro digestion. Therefore, high concentrations of NFC are useful for functional foods delivering satiety instead of oil-soluble bioactives

Exploration of the nutritional and carotenoids profiles of vegetables in Thai cuisine as potential nutritious ingredients

Missing information on plant origin control and nutritional data on herbs, spices and vegetables could lead to sample quality deficit and misusage of the plant database. In this study, twenty vegetables that were collected and managed based on the recommendations of the Department of Agriculture, Ministry of Agriculture and Cooperatives, Thailand, were investigated regarding their proximate mineral, vitamin and carotenoid contents using the standard procedures of the Association of Official Analytical Chemists (AOAC). The results showed that these plants (100 g dry weight) exhibited similar energy levels (337.11–420.48 kcal), which were mainly distributed from high carbohydrate content (21.01–88.17 g), while protein (3.14–66.07 g) and fat (0.00–10.33 g) levels were quite low. As a form of carbohydrate, dietary fiber was found to be high in Cymbopogon citratus (DC.) Stapf (Cy. citratus) and Solanum torvum Sw. (So. torvum) (57.00–59.54 g). Interestingly, Senegalia pennata subsp. insuavis (Lace) Maslin, Seigler & Ebinger (S. pennata) exhibited exceptionally high protein content, which was between 2.3 and 3.1 times higher than its carbohydrates. High mineral contents were detected in S. pennata, Ocimum africanum Lour. (O. africanum), Ocimum basilicum L. (O. basilicum), Ocimum gratissimum L. var. macrophyllum Briq. (O. gratissimum) and Coriandrum sativum L. (Co. sativum), while Mentha cordifolia Opiz ex Fresen (M. cordifolia) was observed to be a good source of vitamin C (381.36–547.47 mg). High carotenoids were mostly found in Eryngium foetidum L. (E. foetidum), O. gratissimum, Co. sativum and O. basilicum (75.23–119.96 mg). Interestingly, the location of sample collection seemed to have minimal effect on the nutritional and carotenoid compositions. The results of this study provide reliable information concerning the nutritional and carotenoid contents in plant sources with control of origin, which could be used in the future for food development with specific nutritional requirements

The Effect of Steaming and Fermentation on Nutritive Values, Antioxidant Activities, and Inhibitory Properties of Tea Leaves

Fermented tea (Cha-miang in Thai) is a local product made by traditional food preservation processes in Northern Thailand that involve steaming fresh tea leaves followed by fermenting in the dark. Information on changes in nutritive values, bioactive compounds, antioxidant activities, and health properties that occur during the steaming and fermenting processes of tea leaves is, however, limited. Changes in nutritive values, phenolics, antioxidant activities, and in vitro health properties through inhibition of key enzymes that control obesity (lipase), diabetes (α-amylase and α-glucosidase), hypertension (angiotensin-converting enzyme (ACE)), and Alzheimer’s disease (cholinesterases (ChEs) and β-secretase (BACE-1)) of fermented tea were compared to the corresponding fresh and steamed tea leaves. Results showed that energy, carbohydrate, and vitamin B1 increased after steaming, while most nutrients including protein, dietary fiber, vitamins (B2, B3, and C), and minerals (Na, K, Ca, Mg, Fe, and Zn) decreased after the steaming process. After fermentation, energy, fat, sodium, potassium, and iron contents increased, while calcium and vitamins (B1, B2, B3, and C) decreased compared to steamed tea leaves. However, the contents of vitamin B1 and iron were insignificantly different between fresh and fermented tea leaves. Five flavonoids (quercetin, kaempferol, cyanidin, myricetin, and apigenin) and three phenolic acids (gallic acid, caffeic acid, and p-coumaric acid) were identified in the tea samples. Total phenolic content (TPC) and antioxidant activities increased significantly after steaming and fermentation, suggesting structural changes in bioactive compounds during these processes. Steamed tea exhibited high inhibition against lipase, α-amylase, and α-glucosidase, while fermented tea possessed high anti-ChE and anti-ACE activities. Fresh tea exhibited high BACE-1 inhibitory activity. Results suggest that tea preparations (steaming and fermentation) play a significant role in the amounts of nutrients and bioactive compounds, which, in turn, affect the in vitro health properties. Knowledge gained from this research will support future investigations on in vivo health properties of fermented tea, as well as promote future food development of fermented tea as a healthy food

Guava and Star gooseberry leaf extracts improve growth performance, innate immunity, intestinal microbial community, and disease resistance in Nile tilapia (Oreochromis niloticus) against Aeromonas hydrophila

The aim of this study was to investigate the antimicrobial activity of guava (Psidium guajava) and star gooseberry (Phyllanthus acidus) leaf extracts and their effects as dietary supplements on the growth performance, intestinal (hindgut) microflora composition, immuno-hematological parameters, and disease resistance of Nile tilapia (Oreochromis niloticus) against Aeromonas hydrophila. Three hundred and sixty Nile tilapia (8.9 ± 0.2 g/fish) were randomly distributed into twelve aquaria (30 fish per aquarium) within a recirculation system. The fish were fed diets supplemented with leaf extracts of guava (GLE), star gooseberry (SGLE), or a mixture of GLE and SGLE (MxLE) at 10 g/kg feed for a duration of 12 weeks, after which their growth performance and intestinal microbial composition were determined. A bacterial challenge with A. hydrophila was performed, and the immune-hematological parameters of the fish and their relative percentage survival (RPS) were determined. Results showed that gallic acid, p-coumaric acid, quercetin, and kaempferol were present in the GLE, SGLE, and MxLE extracts. The GLE and MxLE extracts had significantly higher antimicrobial activity against A. hydrophila than the SGLE. Growth and feed utilization were significantly improved in Nile tilapia after dietary supplementation with all the plant extracts. The intestinal microbial composition of the plant extract groups differed significantly and marginally from the control group. Notably, the post-challenge immune and hematological parameters were considerably enhanced. Furthermore, the RPS of tilapia was significantly higher in diets supplemented with GLE, SGLE, and MxLE extracts compared to the control. These results demonstrated that supplementing the tilapia diets with GLE, SGLE, and MxLE extracts could improve their growth and feed utilization, immuno-hematological response, and disease resistance against A. hydrophila. These plant extracts could also change the composition of the intestinal microbiota. Hence, GLE, SGLE, and MxLE extracts appear to be effective prophylactic and antimicrobial agents for use in Nile tilapia aquaculture