A Molecular Epidemiological Study of Human Parainfluenza 4 in the Western Cape, South Africa

Background Human parainfluenza 4 (HPIV 4) is a recognised cause of acute respiratory infection (ARI). However, there is no published data on the epidemiology of this virus in South Africa. This thesis describes the molecular epidemiology of HPIV 4 over a 4-year period (2014-2017). Respiratory samples from infants, children and adults presenting with respiratory illness in the Western Cape, South Africa were studied. Method A retrospective 4-year study using routine diagnostic samples from patients with ARI was conducted in Western Cape, South Africa. A database search of positive HPIV 4 samples detected by the Seegene Anyplex RV 16 diagnostic assay was extracted. Epidemiological information was recorded to determine age, gender, hospital ward (used as a proxy for disease severity), specimen type (upper or lower respiratory tract) and collection date (to indicate seasonality). To determine genetic evolution, novel primers targeting the haemagglutinin-neuraminidase (HN) in both HPIV 4 subtypes were designed to amplify a 733 bp and 738 bp sequence for HPIV 4A and HPIV 4B respectively. This product was then sequenced and aligned with known reference sequences from GenBank, using BioEdit. These aligned sequences were analysed using the phylogenetic analysis tool, MEGA 6, and Highlighter plots to determine sequence divergence events and evolution. A real-time PCR assay, targeting the phosphoprotein, was developed to rapidly distinguish subtype A and B viruses. Results HPIVs were the 6th most common respiratory viruses detected in diagnostic samples. In all, there were 312/7456 (4.2 %) HPIV 4 positive samples in patients with a median age of 12 months. Males had a higher infection rate. HPIV 4 was the most prevalent of the HPIVs accounting for 47% of all HPIVs. Respiratory infections due to HPIV 4 were seasonal, peaking in autumn and mid-winter (March to August). The overall prevalence of HPIV 4 increased over the study period. Of the HPIV 4-positive samples that were subtyped, 59 were subtype A and 26 subtype B. Both subtypes co-circulated during each season. 71 % of patients who were positive for HPIV 4 were co-infected with one or more additional respiratory virus with Adenovirus (27 %), Human Rhinovirus (23 %) and Bocavirus (19 %) as the most common. HPIV 1 and HPIV 3 were both able to co-infect patients with HPIV 4, but no co-infections with HPIV 2 were detected. Phylogenetic trees constructed using neighbour joining (NJ) method showed that most of the South African HPIV 4 subtypes did not group with the closest significant reference sequences from GenBank. The phylogenetic tree for HPIV 4A revealed 4 genetic groupings. There were many nucleotide changes increasing with time as well as a non-synonymous change in HPIV 4A, at location N161D. HPIV 4B had an amino acid change in location G198R in the HN protein sequenced. Conclusion HPIV 4 with an overall prevalence of 4 % over the study period was identified as a significant cause of ARI in the Western Cape, South Africa. Mono-infection with HPIV4 was associated with severe disease. In hospitalized infants who were HPIV 4 positive, between ¼ to 1/3 were from patients in ICU. Of these almost half (46 %) had HPIV 4 as a single infection. Further studies are needed to fully understand the molecular epidemiology of this infection

Physical capability and the advantages and disadvantages of ageing : Perceptions of older age by men and women in two British cohorts

In an increasingly ageing society, its older members are receiving considerable political and policy attention. However, much remains to be learnt about public perceptions of older age, particularly the views and experiences of older individuals themselves. Drawing on qualitative interviews carried out with members of two British cohorts (N = ??) who have reached the ‘third age’, this paper discusses perceptions of age, focusing particularly on how perceived advantages and disadvantages differ by respondents’ self-reported physical capability. The interviews were carried out in ???? as part of the HALCyon (Healthy Ageing across the Life Course) collaborative research programme. Findings suggest there is some difference in the way older people view aspects of ageing by capability and that although advantages are widely perceived, physical decline and associated health concerns were the overwhelming theme across the conversations. The article concludes by making tentative suggestions to inform the positive ageing agenda and its related policies

Regulation of the Base Excision Repair Pathway by Ubiquitination

Genome integrity is under constant threat from cellular reactive oxygen species generated by endogenous and exogenous mutagens. The base excision repair (BER) pathway consequently plays a crucial role in the repair of DNA base damage, sites of base loss and DNA single strand breaks that can cause genome instability and ultimately the development of human diseases, including premature ageing, neurodegenerative disorders and cancer. Proteins within the base excision repair pathway are increasingly being found to be regulated and controlled by post-translational modifications, and indeed ubiquitination performs a key role in the maintenance of repair protein levels but may also impact on protein activity and cellular localisation. This process is therefore important in maintaining an efficient cellular DNA damage response, and if not accurately controlled, can cause DNA damage accumulation and promote mutagenesis and genomic instability. In this chapter, we will present up-to-date information on the evidence of ubiquitination of base excision repair proteins, the enzymes involved and the molecular and cellular consequences of this process

Producing a Multilingual Audiotape Walking Tour of the Parks Library

Each year thousands of international students enter U.S, colleges and universities for the first time. Typically, these students will tour the library as part of their orientation. Tours, however, are almost always in English, and many of these students have not yet developed the English comprehension skills necessary to fully benefit from the tour. To address this problem the Iowa State University Library developed a multilingual audiotape tour of the Library. The 45 minute tour was first developed and tested in English, then translated into Arabic, Chinese, Japanese, Korean, Malay, and Spanish. A booklet, in English, accompanies the tour and provides maps and basic library information. While many of the problems of producing the English tape were anticipated, problems unique to the translated version were not. Keeping translations down to 45 minutes, knowing if the translations were correct, and recording and editing the tape when the sound technician doesn\u27t understand the language were among the many problems encountered. Evaluations of the English tape were consistently positive. Feedback on the non-English tapes is not yet sufficient to draw conclusions

Planning, Producing, and Implementing a Multilingual Audiotape Walking Tour of the Iowa State University Parks Library

Providing physical orientation to a library remains a standard function of a bibliographic instruction program. Librarians, faced with diminished resources and increased user demands with diverse needs, have responded in creative ways to provide orientation. One such way is the self-guided audiocassette taped library tour. To assist librarians in developing, planning, and implementing self-guided audiocassette walking tours in a variety of languages, a packet of information representing the development of the multilingual audiocassette taped tour is presented. Included here are: the grant proposal, a Parks Library tour script in English, progress and final reports, tour packet provided to library patrons, and guidelines for planning

'The All-Excelling Actress Blushing Bow'd': A Practice-as-Research Exploration of the Performance of Gender in 'Restoration Theatre' by Mid-Eighteenth-Century Actresses

This thesis aims to update and correct the performance of gender in both modern actor-training and modern performance of 'Restoration theatre', by using Practice-as-Research as a methodology to investigate current theories and arguments regarding the performance of gender by mid-eighteenth-century actresses. Practice-as-Research has been used to critically embody traditional archival evidence concerning specific performances by mid-eighteenth-century actresses in order to see how they spoke to the perception of gender at the time. What was discovered was then compared with modern approaches towards performing 'Restoration theatre' to challenge or complement current prevailing narratives in drama schools and the professional stage regarding the performance of gender in 'Restoration theatre'. The research here demonstrates that the performance of gender in plays from the period defined as 'Restoration theatre' did not fit into the binary categories of 'masculine' and 'feminine' as currently taught and performed. Additionally, it shows that though eighteenth-century society would not recognise the terms 'gender play' and 'feminism', the eighteenth-century stage did have performances and characters whose actions and beliefs would now be seen through the lens of these terms. Understanding this not only challenges existing approaches and skills for the performance and teaching of 'Restoration theatre' but introduces new approaches and skills for performers and actor-trainers alike

Structure of isochorismate synthase in complex with magnesium

The structure of the menaquinone-specific isochorismate synthase (MenF) from Escherichia coli has been refined at a resolution of 2.0 Å in complex with magnesium. The magnesium-bound structure has a well defined and organized active site which better represents the active conformation of the enzyme than the currently available structure

A randomized controlled trial and economic evaluation of the Parents Under Pressure Program for parents in substance abuse treatment

Background There is growing interest in the provision of parenting support to substance misusing parents. Methods This pragmatic, multi-center randomized controlled trial compared an intensive one-to-one parenting program (Parents under Pressure, PuP) with Treatment as Usual (TAU) in the UK. Parents were engaged in community-based substance misuse services and were primary caregivers of children less than 2.5 years of age. The primary outcome was child abuse potential, and secondary outcomes included measures of parental emotional regulation assessed at baseline, 6 and 12-months. A prospective economic evaluation was also conducted. Results Of 127 eligible parents, 115 met the inclusion criteria, and subsequently parents were randomly assigned to receive PuP (n = 48) or TAU (n = 52). Child abuse potential was significantly improved in those receiving the PuP program while those in TAU showed a deterioration across time in both intent-to-treat (p < 0.03) and per-protocol analyses (p < 0.01). There was also significant reliable change (recovery/improvement) in 30.6% of the PuP group compared with 10.3% of the TAU group (p < 0.02), and deterioration in 3% compared with 18% (p < 0.02). The probability that the program is cost-effective was approximately 51.8% if decision-makers are willing to pay £1000 for a unit improvement in the primary outcome, increasing to 98.0% at a £20,000 cost-effectiveness threshold for this measure. Conclusions Up to one-third of substance dependent parents of children under 3-years of age can be supported to improve their parenting, using a modular, one-to-one parenting program. Further research is needed

Pennsylvania Folklife Vol. 34, No. 1

• American News in Eighteenth and Nineteenth Century Swiss Almanacs: An Overview • The Stuff of Which Folksongs are Made: Dialect Poetry of Lina Somer (1862-1932) • Word Gain and Loss in the English of the Pennsylvania Germans • The Autograph Album: A Victorian Girl\u27s Best Friend • Cobwebs on My Mind: Untangling Family Relationships • Aldes un Neieshttps://digitalcommons.ursinus.edu/pafolklifemag/1105/thumbnail.jp