Implicit attitude toward caregiving: The moderating role of adult attachment styles

Attachment and caregiving are separate motivational systems that share the common evolutionary purpose of favoring child security. In the goal of studying the processes underlying the transmission of attachment styles, this study focused on the role of adult attachment styles in shaping preferences toward particular styles of caregiving. We hypothesized a correspondence between attachment and caregiving styles: we expect an individual to show a preference for a caregiving behavior coherent with his/her own attachment style, in order to increase the chance of passing it on to offspring. We activated different representations of specific caregiving modalities in females, by using three videos in which mothers with different Adult Attachment states of mind played with their infants. Participants' facial expressions while watching were recorded and analyzed with FaceReader software. After each video, participants' attitudes toward the category "mother" were measured, both explicitly (semantic differential) and implicitly (single target-implicit association task, ST-IAT). Participants' adult attachment styles (experiences in close relationships revised) predicted attitudes scores, but only when measured implicitly. Participants scored higher on the ST-IAT after watching a video coherent with their attachment style. No effect was found on the facial expressions of disgust. These findings suggest a role of adult attachment styles in shaping implicit attitudes related to the caregiving system

Like grandparents, like parents: Empirical evidence and psychoanalytic thinking on the transmission of parenting styles

The authors discuss the issue of intergenerational transmission of parenting from an empirical and psychoanalytic perspective. After presenting a framework to explain their conception of parenting, they describe intergenerational transmission of parenting as a key to interpreting and eventually changing parenting behaviors. Then they present (1) the empirical approach aimed at determining if there is actually a stability across generations that contributes to harsh parenting and eventually maltreatment and (2) the psyphoanalytic thinking that seeks to explain the continuity in terms of representations and clinical phenomena. The authors also discuss the relationship between the attachment and the caregiving systems and hypothesize a common base for the two systems in childhood experience. Finally, they propose the psychoanalytic perspective as a fruitful theoretical framework to integrate the evidence for the neurophysiological mediators and moderators of intergenerational transmission. Psychoanalytically informed research can provide clinically relevant insights and hypotheses to be tested

Insight into phenotypic and genotypic differences between vaginal lactobacillus crispatus bc5 and lactobacillus gasseri bc12 to unravel nutritional and stress factors influencing their metabolic activity

The vaginal microbiota, normally characterized by lactobacilli presence, is crucial for vaginal health. Members belonging to L. crispatus and L. gasseri species exert crucial protective functions against pathogens, although a total comprehension of factors that influence their dominance in healthy women is still lacking. Here we investigated the complete genome sequence and comprehensive phenotypic profile of L. crispatus strain BC5 and L. gasseri strain BC12, two vaginal strains featured by anti-bacterial and anti-viral activities. Phenotype microarray (PM) results revealed an improved capacity of BC5 to utilize different carbon sources as compared to BC12, although some specific carbon sources that can be associated to the human diet were only metabolized by BC12, i.e. uridine, amygdalin, tagatose. Additionally, the two strains were mostly distinct in the capacity to utilize the nitrogen sources under analysis. On the other hand, BC12 showed tolerance/resistance towards twice the number of stressors (i.e. antibiotics, toxic metals etc.) with respect to BC5. The divergent phenotypes observed in PM were supported by the identification in either BC5 or BC12 of specific genetic determinants that were found to be part of the core genome of each species. The PM results in combination with comparative genome data provide insights into the possible environmental factors and genetic traits supporting the predominance of either L. crispatus BC5 or L. gasseri BC12 in the vaginal niche, giving also indications for metabolic predictions at the species level

β\beta-NMR of Isolated 8^{8}Li+^{+} Implanted into a Thin Copper Film

Depth-controlled $\beta$-NMR was used to study highly spin-polarized $^8$Li in a Cu film of thickness 100 nm deposited onto a MgO substrate. The positive Knight Shifts and spin relaxation data show that $^8$Li occupies two sites at low temperatures, assigned to be the substitutional ($S$) and octahedral ($O$) interstitial sites. Between 50 to 100 K, there is a site change from $O$ to $S$. The temperature dependence of the Knight shifts and spin-lattice relaxation rates at high temperatures, i.e. when all the Li are in the $S$ site, is consistent with the Korringa Law for a simple metal.Comment: Accepted for publication in Phys. Rev.

Hyperfine Fields in an Ag/Fe Multilayer Film Investigated with 8Li beta-Detected Nuclear Magnetic Resonance

Low energy $\beta$-detected nuclear magnetic resonance ($\beta$-NMR) was used to investigate the spatial dependence of the hyperfine magnetic fields induced by Fe in the nonmagnetic Ag of an Au(40 \AA)/Ag(200 \AA)/Fe(140 \AA) (001) magnetic multilayer (MML) grown on GaAs. The resonance lineshape in the Ag layer shows dramatic broadening compared to intrinsic Ag. This broadening is attributed to large induced magnetic fields in this layer by the magnetic Fe layer. We find that the induced hyperfine field in the Ag follows a power law decay away from the Ag/Fe interface with power $-1.93(8)$, and a field extrapolated to $0.23(5)$ T at the interface.Comment: 5 pages, 4 figure. To be published in Phys. Rev.

Metabolic Variability of a Multispecies Probiotic Preparation Impacts on the Anti-inflammatory Activity

Background: In addition to strain taxonomy, the ability of probiotics to confer beneficial effects on the host rely on a number of additional factors including epigenetic modulation of bacterial genes leading to metabolic variability and might impact on probiotic functionality. Aims: To investigate metabolism and functionality of two different batches of a probiotic blend commercialized under the same name in Europe in models of intestinal inflammation. Methods: Boxes of VSL#3, a probiotic mixture used in the treatment of pouchitis, were obtained from pharmacies in UK subjected to metabolomic analysis and their functionality tested in mice rendered colitis by treatment with DSS or TNBS. Results: VSL#3-A (lot DM538), but not VSL#3-B (lot 507132), attenuated "clinical" signs of colitis in the DSS and TNBS models. In both models, VSL#3-A, but not VSL#3-B, reduced macroscopic scores, intestinal permeability, and expression of TNFα, IL-1β, and IL-6 mRNAs, while increased the expression of TGFβ and IL-10, occludin, and zonula occludens-1 (ZO-1) mRNAs and shifted colonic macrophages from a M1 to M2 phenotype (P < 0.05 vs. TNBS). In contrast, VSL#3-B failed to reduce inflammation, and worsened intestinal permeability in the DSS model (P < 0.001 vs. VSL#3-A). A metabolomic analysis of the two formulations allowed the identification of two specific patterns, with at least three-folds enrichment in the concentrations of four metabolites, including 1-3 dihydroxyacetone (DHA), an intermediate in the fructose metabolism, in VSL#3-B supernatants. Feeding mice with DHA, increased intestinal permeability. Conclusions: Two batches of a commercially available probiotic show divergent metabolic activities. DHA, a product of probiotic metabolism, increases intestinal permeability, highlighting the complex interactions between food, microbiota, probiotics, and intestinal inflammation

Local Magnetic Properties of a Monolayer of Mn12 Single Molecule Magnets

The magnetic properties of a monolayer of Mn12 single molecule magnets grafted onto a Si substrate have been investigated using depth-controlled $\beta$-detected nuclear magnetic resonance. A low energy beam of spin polarized radioactive 8Li was used to probe the local static magnetic field distribution near the Mn12 monolayer in the Si substrate. The resonance linewidth varies strongly as a function of implantation depth as a result of the magnetic dipolar fields generated by the Mn12 electronic magnetic moments. The temperature dependence of the linewidth indicates that the magnetic properties of the Mn12 moments in this low dimensional configuration differ from bulk Mn12.Comment: 6 pages, 4 figure

Giant Vortices Below the Surface of NbSe2_2 Detected Using Low Energy β\beta-NMR

A low energy radioactive beam of polarized $^8$Li has been used to observe the vortex lattice near the surface of superconducting NbSe$_2$. The inhomogeneous magnetic field distribution associated with the vortex lattice was measured using depth-resolved $\beta$-detected NMR. Below $T_c$ one observes the characteristic lineshape for a triangular vortex lattice which depends on the magnetic penetration depth and vortex core radius. The size of the vortex core varies strongly with magnetic field. In particular in a low field of 10.8 mT the core radius is much larger than the coherence length. The possible origin of these giant vortices is discussed.Comment: 5 pages, 3 figures. Submitted to Phys. Rev. Let