8,569 research outputs found
Dual apoptotic DNA fragmentation system in the fly: Drep2 is a novel nuclease of which activity is inhibited by Drep3
AbstractDNA fragmentation is the hallmark of apoptotic cells and mainly mediated by the DNA fragmentation factor DFF40(CAD)/DFF45(ICAD). DFF40 is a novel nuclease, whereas DFF45 is an inhibitor that can suppress the nuclease activity. Apoptotic DNA fragmentation in the fly is controlled by four DFF-related proteins, known as Drep1, 2, 3 and 4. However, the functions of Drep2 and Drep3 are totally unknown. Here, we found that Drep2 is a novel nuclease whose activity is inhibited by Drep3 through a tight interaction with the CIDE domain. Our results suggest that the fly has dual apoptotic DNA fragmentation systems: Drep1: Drep4 and Drep2: Drep3 complexes.Structured summary of protein interactionsDrep2 CIDE and Drep-3 CIDE bind by blue native page (View interaction)Drep2 CIDE and Drep-3 CIDE bind by molecular sieving (View interaction
The structure of gauge-invariant ideals of labelled graph -algebras
In this paper, we consider the gauge-invariant ideal structure of a
-algebra associated to a set-finite,
receiver set-finite and weakly left-resolving labelled space
, where is a labelling map assigning
an alphabet to each edge of the directed graph with no sinks. Under the
assumption that an accommodating set is closed under taking
relative complement, it is obtained that there is a one to one correspondence
between the set of all hereditary saturated subsets of and the
gauge-invariant ideals of . For this, we
introduce a quotient labelled space arising
from an equivalence relation on and show the existence
of the -algebra generated by a
universal representation of . Also the
gauge-invariant uniqueness theorem for is
obtained.
For simple labelled graph -algebras
, where is the
smallest accommodating set containing all the generalized vertices, it is
observed that if for each vertex of , a generalized vertex is
finite for some , then is simple if
and only if is strongly cofinal and
disagreeable. This is done by examining the merged labelled graph
of and the common properties that
and
share
General interaction mode of CIDE:CIDE complex revealed by a mutation study of the Drep2 CIDE domain
AbstractThe CIDE domain is a well known protein–protein interaction module that is initially detected at the apoptotic DNA fragmentation factor (DFF40/45). The interaction mechanism via the CIDE domain is not well understood. To elucidate CIDE domain mediated interactions in the apoptotic DNA fragmentation system, we conducted biochemical and mutational studies and found that the surface of CIDE domains can be divided into an acidic side and a basic side. In addition, a mutagenesis study revealed that the basic surface side of Drep2 CIDE is involved in the interaction with the acidic surface side of Drep1 CIDE and Drep3 CIDE. Our research supports the idea that a charge–charge interaction might be the general interaction mode of the CIDE:CIDE interaction.Structured summary of protein interactionsDrep2andDrep2bindbymolecular sieving(View Interaction:1,2)Drep1andDrep2bindbymolecular sieving(View interaction)Drep3andDrep2bindbymolecular sieving(View interaction)Drep2andDrep3bindbyblue native page(View interaction)Drep2andDrep1bindbyblue native page(View interaction
Optimal design of quadratic electromagnetic exciter
The vibration acceleration of collecting plates, which is the core indicator of rapping performance in an electrostatic precipitator’s vibration rapping process, is determined by magnetic force of a quadratic electromagnetic exciter. The larger exciter provides the larger magnetic force, but the installation space for the exciter is limited. Accordingly, this paper presents the optimal design of quadratic electromagnetic exciter to maximize the magnetic force with constraint that the size of exciter is constant. A design optimization problem was formulated in order to find the quadratic electromagnetic exciter shape parameters that maximized the magnetic force. The magnetic force of the quadratic electromagnetic exciter was evaluated using the commercial electromagnetic analysis software “MAXWELL”. For efficient design, we employed metamodel-based design optimization using design of experiments (DOE), metamodels, and an optimization algorithm equipped in PIAnO (Process Integration, Automation and Optimization), a commercial PIDO (Process Integration and Design Optimization) tool. Using the proposed design approach, the optimal magnetic force was increased by 1.68 % compared to the initial one. This result demonstrates the effectiveness of the established analysis and design procedure for the quadratic electromagnetic exciter
Efficiency Analysis of Major Container Ports in Asia: Using DEA and Shannon’s Entropy
This paper attempts to evaluate performance (i.e. efficiency) of Asias container ports. Measurement of the ports performance is critical to increase the competitiveness of maritime transport, ultimately leading to one nations competitive advantages over other countries. Data Envelopment Analysis (DEA), which is a non-parametric method widely used for assessing efficiency of units which have similar characteristics, was selected to analyse the data. Due to the limitations of the DEA method producing diverse results according to different models, and to the complexities of choosing a specific model among several DEA models, Shannons Entropy was also employed. By including Shannons Entropy, the efficiency results calculated from each model were integrated in order to rank the ports. The results in this study will provide port managers with valuable information in order to understand the current status of Asias container ports in terms of their efficiency
Dynamical mean-field theory of Hubbard-Holstein model at half-filling: Zero temperature metal-insulator and insulator-insulator transitions
We study the Hubbard-Holstein model, which includes both the
electron-electron and electron-phonon interactions characterized by and
, respectively, employing the dynamical mean-field theory combined with
Wilson's numerical renormalization group technique. A zero temperature phase
diagram of metal-insulator and insulator-insulator transitions at half-filling
is mapped out which exhibits the interplay between and . As () is
increased, a metal to Mott-Hubbard insulator (bipolaron insulator) transition
occurs, and the two insulating states are distinct and can not be adiabatically
connected. The nature of and transitions between the three states are
discussed.Comment: 5 pages, 4 figures. Submitted to Physical Review Letter
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