4 research outputs found
Chromosome-Centric Human Proteome Project Allies with Developmental Biology: A Case Study of the Role of Y Chromosome Genes in Organ Development
<i>DDX3Y</i>, a Male-Specific Region of Y Chromosome Gene, May Modulate Neuronal Differentiation
Although
it is apparent that chromosome complement mediates sexually
dimorphic expression patterns of some proteins that lead to functional
differences, there has been insufficient evidence following the manipulation
of the male-specific region of the Y chromosome (MSY) gene expression
during neural development. In this study, we profiled the expression
of 23 MSY genes and 15 of their X-linked homologues during neural
cell differentiation of NTERA-2 human embryonal carcinoma cell line
(NT2) cells in three different developmental stages using qRT-PCR,
Western blotting, and immunofluorescence. The expression level of
12 Y-linked genes significantly increased over neural differentiation,
including <i>RBMY1</i>, <i>EIF1AY</i>, <i>DDX3Y</i>, <i>HSFY1</i>, <i>BPY2</i>,<i> PCDH11Y</i>, <i>UTY</i>, <i>RPS4Y1</i>, <i>USP9Y</i>, <i>SRY</i>, <i>PRY</i>, and <i>ZFY</i>. We showed that siRNA-mediated knockdown of DDX3Y, a
DEAD box RNA helicase enzyme, in neural progenitor cells impaired
cell cycle progression and increased apoptosis, consequently interrupting
differentiation. Label-free quantitative shotgun proteomics based
on a spectral counting approach was then used to characterize the
proteomic profile of the cells after <i>DDX3Y</i> knockdown.
Among 917 reproducibly identified proteins detected, 71 proteins were
differentially expressed following <i>DDX3Y</i> siRNA treatment
compared with mock treated cells. Functional grouping indicated that
these proteins were involved in cell cycle, RNA splicing, and apoptosis,
among other biological functions. Our results suggest that MSY genes
may play an important role in neural differentiation and demonstrate
that <i>DDX3Y</i> could play a multifunctional role in neural
cell development, probably in a sexually dimorphic manner
<i>DDX3Y</i>, a Male-Specific Region of Y Chromosome Gene, May Modulate Neuronal Differentiation
Although
it is apparent that chromosome complement mediates sexually
dimorphic expression patterns of some proteins that lead to functional
differences, there has been insufficient evidence following the manipulation
of the male-specific region of the Y chromosome (MSY) gene expression
during neural development. In this study, we profiled the expression
of 23 MSY genes and 15 of their X-linked homologues during neural
cell differentiation of NTERA-2 human embryonal carcinoma cell line
(NT2) cells in three different developmental stages using qRT-PCR,
Western blotting, and immunofluorescence. The expression level of
12 Y-linked genes significantly increased over neural differentiation,
including <i>RBMY1</i>, <i>EIF1AY</i>, <i>DDX3Y</i>, <i>HSFY1</i>, <i>BPY2</i>,<i> PCDH11Y</i>, <i>UTY</i>, <i>RPS4Y1</i>, <i>USP9Y</i>, <i>SRY</i>, <i>PRY</i>, and <i>ZFY</i>. We showed that siRNA-mediated knockdown of DDX3Y, a
DEAD box RNA helicase enzyme, in neural progenitor cells impaired
cell cycle progression and increased apoptosis, consequently interrupting
differentiation. Label-free quantitative shotgun proteomics based
on a spectral counting approach was then used to characterize the
proteomic profile of the cells after <i>DDX3Y</i> knockdown.
Among 917 reproducibly identified proteins detected, 71 proteins were
differentially expressed following <i>DDX3Y</i> siRNA treatment
compared with mock treated cells. Functional grouping indicated that
these proteins were involved in cell cycle, RNA splicing, and apoptosis,
among other biological functions. Our results suggest that MSY genes
may play an important role in neural differentiation and demonstrate
that <i>DDX3Y</i> could play a multifunctional role in neural
cell development, probably in a sexually dimorphic manner
<i>DDX3Y</i>, a Male-Specific Region of Y Chromosome Gene, May Modulate Neuronal Differentiation
Although
it is apparent that chromosome complement mediates sexually
dimorphic expression patterns of some proteins that lead to functional
differences, there has been insufficient evidence following the manipulation
of the male-specific region of the Y chromosome (MSY) gene expression
during neural development. In this study, we profiled the expression
of 23 MSY genes and 15 of their X-linked homologues during neural
cell differentiation of NTERA-2 human embryonal carcinoma cell line
(NT2) cells in three different developmental stages using qRT-PCR,
Western blotting, and immunofluorescence. The expression level of
12 Y-linked genes significantly increased over neural differentiation,
including <i>RBMY1</i>, <i>EIF1AY</i>, <i>DDX3Y</i>, <i>HSFY1</i>, <i>BPY2</i>,<i> PCDH11Y</i>, <i>UTY</i>, <i>RPS4Y1</i>, <i>USP9Y</i>, <i>SRY</i>, <i>PRY</i>, and <i>ZFY</i>. We showed that siRNA-mediated knockdown of DDX3Y, a
DEAD box RNA helicase enzyme, in neural progenitor cells impaired
cell cycle progression and increased apoptosis, consequently interrupting
differentiation. Label-free quantitative shotgun proteomics based
on a spectral counting approach was then used to characterize the
proteomic profile of the cells after <i>DDX3Y</i> knockdown.
Among 917 reproducibly identified proteins detected, 71 proteins were
differentially expressed following <i>DDX3Y</i> siRNA treatment
compared with mock treated cells. Functional grouping indicated that
these proteins were involved in cell cycle, RNA splicing, and apoptosis,
among other biological functions. Our results suggest that MSY genes
may play an important role in neural differentiation and demonstrate
that <i>DDX3Y</i> could play a multifunctional role in neural
cell development, probably in a sexually dimorphic manner