Correction: Application of a qPCR Assay in the Investigation of Susceptibility to Malaria Infection of the M and S Molecular Forms of An. gambiae s.s. in Cameroon.

[This corrects the article DOI: 10.1371/journal.pone.0054820.]

Bland-Altman plot.

<p>The plot shows the difference of infection prevalence between the M and S molecular forms in mosquito populations collected in allopatric (blue triangles) and sympatric (red circles) conditions. Each triangle or circle represents an experiment for which M and S mosquitoes were fed on a same blood donor. A positive value indicates a higher infection rate for the M form, and a negative one a higher infection rate for the S form. The M form was more infected than the S one for 76% (19/25) of feedings, and the difference is significant (X² = 11.52, <i>P</i><0.001).</p

Comparison of standard curves between qPCR assays targeting the cox1 and SSU rRNA genes.

<p>Composite plots were obtained by linear regression analysis of Ct values versus log₁₀ copy numbers of cultured parasites in DNA standards (6 to 6×10⁴ genomes/µl). The slopes were generated from 53 standard curves for the cox1 assay (blue diamonds) and 38 for the SSU rRNA assay (red circles), respectively. Error bars indicate the standard deviation. The SSU rRNA qPCR assay was not reproducible in reactions containing 6 genomes and was not taken into account in the plot.</p

Results of the meta-analysis on infection prevalence for the different combinations of mosquito populations.

*<p>The P value was computed using random effects model. Significant values are indicated in bold italics.</p

Results of the meta-analysis on infection intensity for the different combinations of mosquito populations.

*<p>The P value was computed using random effects model.</p

Standard curve of qPCR using serial dilutions of DNAs from cultured parasites.

<p>A, Standard curve was obtained by linear regression analysis of Ct values versus log₁₀ copy number of cultured parasites in DNA standards (6 to 6×10⁴ genomes/µl). The slope was calculated from 53 independent reactions for each serial dilution. Errors bars show the standard deviation. B, Calibration curve for absolute quantification representing the starting concentrations of DNA standards (N0), expressed as arbitrary fluorescent units, versus log₁₀ input copy numbers of cultured parasites (6 to 6×10⁴ genomes/µl). N0 were calculated for each sample of the serial dilutions using the LinRegPCR software. The curve shows a good correlation of the N0 values with the estimate parasite number in serial dilutions (slope = 0.952, and R²>0.998). The calibration curve was used to convert the starting concentration of parasites in each mosquito midgut into the number of genomes/µl.</p

Taxonomic hierarchical classification of the major bacterial groups for each locality.

<p>Bray Curtis similarities were calculated for genera abundance and the dendrogram was constructed using the “complete” method. The bubble plot schematically represents major genera abundances (>1%). Nkolb (Nkolbisson), Mvan, Nkdom (Nkolondon), and Nkum (Nkolkumu) indicate the locality where the mosquitoes came from.</p