Cotrimoxazole reduces systemic inflammation in HIV infection by altering the gut microbiome and immune activation

Wellcome TrustCanadian Institutes of Health ResearchMedical Research CouncilDepartment for International Development under MRC/DFID Concordat agreement and EDCTP2 programme supported by the European UnionMRC Clinical Trials Unit at UC

Completion of Hepatitis C Virus Replication Cycle in Heterokaryons Excludes Dominant Restrictions in Human Non-liver and Mouse Liver Cell Lines

Hepatitis C virus (HCV) is hepatotropic and only infects humans and chimpanzees. Consequently, an immunocompetent small animal model is lacking. The restricted tropism of HCV likely reflects specific host factor requirements. We investigated if dominant restriction factors expressed in non-liver or non-human cell lines inhibit HCV propagation thus rendering these cells non-permissive. To this end we explored if HCV completes its replication cycle in heterokaryons between human liver cell lines and non-permissive cell lines from human non-liver or mouse liver origin. Despite functional viral pattern recognition pathways and responsiveness to interferon, virus production was observed in all fused cells and was only ablated when cells were treated with exogenous interferon. These results exclude that constitutive or virus-induced expression of dominant restriction factors prevents propagation of HCV in these cell types, which has important implications for HCV tissue and species tropism. In turn, these data strongly advocate transgenic approaches of crucial human HCV cofactors to establish an immunocompetent small animal model

Electrochemically synthesized polymers in molecular imprinting for chemical sensing

This critical review describes a class of polymers prepared by electrochemical polymerization that employs the concept of molecular imprinting for chemical sensing. The principal focus is on both conducting and nonconducting polymers prepared by electropolymerization of electroactive functional monomers, such as pristine and derivatized pyrrole, aminophenylboronic acid, thiophene, porphyrin, aniline, phenylenediamine, phenol, and thiophenol. A critical evaluation of the literature on electrosynthesized molecularly imprinted polymers (MIPs) applied as recognition elements of chemical sensors is presented. The aim of this review is to highlight recent achievements in analytical applications of these MIPs, including present strategies of determination of different analytes as well as identification and solutions for problems encountered

Update on gemstone mining in Luc Yen, Vietnam

In 1987, gems were discovered in the Luc Yen area of Vietnam's Yen Bai province. Ruby, sapphire, and spinel from primary and secondary deposits are the most valuable of these; tourmaline, gem-quality feldspar, pargasite, and humite are also mined there. As a result of this discovery, the Luc Yen district has become Vietnam's most important gemstone-trading center over the past 30 years. The markets of Yen The, the capital of Luc Yen, offer an additional assortment of natural gems from Vietnam and elsewhere (including peridot, beryl, aquamarine, topaz, and quartz); synthetic gems are also found in these markets

Pink and red spinels in marble: trace elements, oxygen isotopes, and sources

The oxygen isotopic composition of pink to red and other colored spinels hosted by marbles worldwide have been combined with trace elements investigated by electron microprobe analysis (EMPA). For the first time, this database enables the characterization of the geographic origin of the main productive and historical sources of gem spinel. Three consistent sets of delta O-18 values were found for primary and placer deposits: (1) between 5.6 and 8.6% for the historical Kuh-i-Lal spinels from Tajikistan; (2) from 12.1 to 18.5% for spinels from An Phu and Cong Troi in Vietnam, and Namya from Myanmar; and (3) between 19.0 and 24.2% for spinels from Paigutan in Nepal, Kiswila and Ipanko in Tanzania, Pamreso in Kenya, Mogok in Myanmar, and An Phu in Vietnam. The variation in the delta O-18 values for most of the deposits can be related to the buffering of the metamorphic fluids by the oxygen isotope composition of the impure marbles. The delta O-18 values of Kuh-i-Lal spinels result from a metasomatic skarn process between granitic intrusives and marble. Trace elements, such as Fe-Zn-Cr-V-Ti, presented in various new chemical discrimination diagrams allow for the differentiation of all the pink to red spinels. The slight overlaps observed for some chemical domains can be resolved by combination with the O-isotope composition of spinel. The present study shows that it is possible to discriminate gem spinels hosted by marbles from different geographic regions of the world and provides new insights into the traceability of historical spinels such as those from Kuh-i-Lal

Ruby deposits : a review and geological classification

Corundum is not uncommon on Earth but the gem varieties of ruby and sapphire are relatively rare. Gem corundum deposits are classified as primary and secondary deposits. Primary deposits contain corundum either in the rocks where it crystallized or as xenocrysts and xenoliths carried by magmas to the Earth's surface. Classification systems for corundum deposits are based on different mineralogical and geological features. An up-to-date classification scheme for ruby deposits is described in the present paper. Ruby forms in mafic or felsic geological environments, or in metamorphosed carbonate platforms but it is always associated with rocks depleted in silica and enriched in alumina. Two major geological environments are favorable for the presence of ruby: (1) amphibolite to medium pressure granulite facies metamorphic belts and (2) alkaline basaltic volcanism in continental rifting environments. Primary ruby deposits formed from the Archean (2.71 Ga) in Greenland to the Pliocene (5 Ma) in Nepal. Secondary ruby deposits have formed at various times from the erosion of metamorphic belts (since the Precambrian) and alkali basalts (from the Cenozoic to the Quaternary). Primary ruby deposits are subdivided into two types based on their geological environment of formation: (Type I) magmatic-related and (Type II) metamorphic-related. Type I is characterized by two sub-types, specifically Type IA where xenocrysts or xenoliths of gem ruby of metamorphic (sometimes magmatic) origin are hosted by alkali basalts (Madagascar and others), and Type IB corresponding to xenocrysts of ruby in kimberlite (Democratic Republic of Congo). Type II also has two sub-types; metamorphic deposits sensu stricto (Type IIA) that formed in amphibolite to granulite facies environments, and metamorphic-metasomatic deposits (Type IIB) formed via high fluid-rock interaction and metasomatism. Secondary ruby deposits, i.e., placers are termed sedimentary-related (Type III). These placers are hosted in sedimentary rocks (soil, rudite, arenite, and silt) that formed via erosion, gravity effect, mechanical transport, and sedimentation along slopes or basins related to neotectonic motions and deformation

¹⁸O/¹⁶O and V/Cr ratios in gem tsavorites from the Neoproterozoic Mozambique metamorphic belt: a clue towards their origins?

The combination of oxygen isotope composition with V–Cr–Mn trace element concentrations of V-bearing garnets (tsavorites) originating from the main deposits of the Neoproterozoic Mozambique Metamorphic Belt is reported for the first time. The database enables the identification of the geological and geographical sources of the main productive areas from northern and southern Tanzania, Kenya, and Madagascar. Three consistent sets of δ<sup>18</sup>O values between 9.5‰ and 11.0‰, 11.6‰ and 14.5‰, and 15.5‰ and 21.1‰ have been recognized for primary deposits hosted in graphitic gneisses related to the Neoproterozic metasedimentary series. The δ<sup>18</sup>O value of tsavorite is a good tracer of the environment of its formation; the δ<sup>18</sup>O of the fluid in equilibrium with tsavorite was buffered by the host rock during metamorphism and fluid-rock interaction. This study is a first step in characterizing the geochemistry of gem tsavorite from most of the deposits and occurrences worldwide

Lithostratigraphic and structural controls of 'tsavorite' deposits at Lemshuku, Merelani area, Tanzania

The first study of the Lemshuku 'tsavorite' mining district is presented. From bottom to top, the lithostratigraphic column corresponds to a metasedimentary sequence composed of quartzite, fine-grained graphitic gneiss, kyanite-graphite gneiss, biotite-almandine gneiss, metasomatized graphitic gneiss and dolomitic marble. 'Tsavorite' occurs in quartz veins and rarely as nodular concretions. Two factors control mineralization: (1) lithostratigraphy, with 'tsavorite' in association with pyrite and graphite confined to quartz veins within the metasomatized graphitic gneiss: and (2) structure, with the mineralized veins characteristically controlled by tight isoclinal folds associated with shearing