The Effect of Service Quality and Performance of Cooperative on Cooperative Image and Its Impact to the Satisfaction of Cooperative Member in the Central Sulawesi Province

This study aims to analyze the effect of service quality and performance of cooperatives on the cooperative image and its impact to the satisfaction of cooperative member in Central Sulawesi Province. The relation between the research variables corroborated by some marketing experts opinions, among others, Kotler (2013), Bateson (2005), Tjiptono (2014; 266), Rust, et al (1996) in Tjiptono (2014; 268), Lovelock (1988) in Tjiptono (2014; 268), Grönroos in Tjiptono (2014: 268) and Harrison in Iman (2010). The populations in this research are members of the cooperative that received the rating "quality" of the Department of Cooperatives, Micro, Small and Medium Enterprises of Central Sulawesi province in 2015. The number of cooperatives received the rating of "Quality" in 2015 as many as 33 units of cooperative. Total of population in this study are 7663 members of the cooperative, sampling techniques performed is Proportionate Random Sampling of 7663 members of the cooperative, the number of the sample are150 members of cooperative as well as respondents. The data collection was conducted using questionnaires and data collected are tested for their validity, reliability, and normality. Research hypothesis are proven by the path analysis. The results of the study are summarized in the conclusion: 1) Quality of Service significantly and positively impact on the image of cooperatives in the Central Sulawesi Province. 2) The Performance of Cooperative has significant and positive impact on the image of cooperatives in the Province of Central Sulawesi. 3) Service Quality has significant and positive impact on Cooperative Members satisfaction in Central Sulawesi province. 4) Cooperative Performance has significant and positive impact on satisfaction Cooperative Members in Central Sulawesi province. 5) The image of Cooperative significant and positive impact on satisfaction Cooperative Members in Central Sulawesi province. 6) Service quality has no significant effect on the satisfaction of Cooperative Members in Central Sulawesi mediated by Citra Cooperative. 7) Performance of the Cooperative significantly affect the satisfaction of Cooperative Members in Central Sulawesi which mediated by Citra Cooperative. Keywords: Quality, Performance, Image and Satisfactio

Molecular phylogeny of mangroves V. Analysis of genome relationships in mangrove species using RAPD and RFLP markers.

Abstract DNA from pooled leaf samples of 11 true major mangrove, three true minor mangrove, two mangrove associate, two mangrove parasite, three terrestrial and one cultivated species were isolated for the present study. In total, 198 random amplified polymorphic DNAs (RAPDs) and 180 restriction fragment length polymorphism (RFLP) loci were scored by using ten primers and 14 enzyme-probe combinations respectively. The polymorphism observed for these markers revealed a high degree of genetic diversity in mangroves at both inter-specific or inter-generic levels. A dendrogram, constructed after pooling both RAPD and RFLP data, using a similarity index was analysed for genome relationships among these species. The dendrogram showed clustering of all the major mangroves, except for Nypa fruticans (Arecaceae), into one group. All species under the tribe Rhizophorae formed a subcluster, to which Xylocarpus granatum was found to be the most closesly related species. The clustering pattern implied that Excoecaria agallocha and Acanthus ilicifolius should be considered as true minor mangroves. The present study also provided molecular data favouring the separation of Avicennia spp. from the Verbenaceae to create a monotypic family the Avicenniaceae. The separation of »iscum orientale into the Viscaceae was also favoured

Seminar sejarah nasional v subtema sejarah kesenian

Buku ini berisi tulisan-tulisan dari Seminar sejarah nasional v dengan subtema sejarah kesenian

De novo assembly and transcriptome analysis of five major tissues of Jatropha curcas L. using GS FLX titanium platform of 454 pyrosequencing

<p>Abstract</p> <p>Background</p> <p><it>Jatropha curcas </it>L. is an important non-edible oilseed crop with promising future in biodiesel production. However, factors like oil yield, oil composition, toxic compounds in oil cake, pests and diseases limit its commercial potential. Well established genetic engineering methods using cloned genes could be used to address these limitations. Earlier, 10,983 unigenes from Sanger sequencing of ESTs, and 3,484 unique assembled transcripts from 454 pyrosequencing of uncloned cDNAs were reported. In order to expedite the process of gene discovery, we have undertaken 454 pyrosequencing of normalized cDNAs prepared from roots, mature leaves, flowers, developing seeds, and embryos of <it>J. curcas</it>.</p> <p>Results</p> <p>From 383,918 raw reads, we obtained 381,957 quality-filtered and trimmed reads that are suitable for the assembly of transcript sequences. <it>De novo </it>contig assembly of these reads generated 17,457 assembled transcripts (contigs) and 54,002 singletons. Average length of the assembled transcripts was 916 bp. About 30% of the transcripts were longer than 1000 bases, and the size of the longest transcript was 7,173 bases. BLASTX analysis revealed that 2,589 of these transcripts are full-length. The assembled transcripts were validated by RT-PCR analysis of 28 transcripts. The results showed that the transcripts were correctly assembled and represent actively expressed genes. KEGG pathway mapping showed that 2,320 transcripts are related to major biochemical pathways including the oil biosynthesis pathway. Overall, the current study reports 14,327 new assembled transcripts which included 2589 full-length transcripts and 27 transcripts that are directly involved in oil biosynthesis.</p> <p>Conclusion</p> <p>The large number of transcripts reported in the current study together with existing ESTs and transcript sequences will serve as an invaluable genetic resource for crop improvement in jatropha. Sequence information of those genes that are involved in oil biosynthesis could be used for metabolic engineering of jatropha to increase oil content, and to modify oil composition.</p

Last Men Standing: Chlamydatus Portraits and Public Life in Late Antique Corinth

Notable among the marble sculptures excavated at Corinth are seven portraits of men wearing the long chlamys of Late Antique imperial office. This unusual costume, contemporary portrait heads, and inscribed statue bases all help confirm that new public statuary was created and erected at Corinth during the 4th and 5th centuries. These chlamydatus portraits, published together here for the first time, are likely to represent the Governor of Achaia in his capital city, in the company of local benefactors. Among the last works of the ancient sculptural tradition, they form a valuable source of information on public life in Late Antique Corinth

Cloning and Characterization of a Gene Encoding Ubiquitin Conjugating Enzyme from the Mangrove Species, <i>Avicennia marina </i>(Forsk.) Vierh.

164-169Covalent attachment of ubiquitin has been implicated in mediating proteolysis of the cellular proteins by Ubiquitin- proteasome pathway. Ubiquitin activating enzyme (E1), ubiquitin conjugating enzyme (E2), and ubiquitin protein ligase (E3) are the three enzymes involved in this process. This paper reports the isolation of a gene that codes for the ubiquitin conjugating enzyme in Avicennia marina (AmUBC2), and regulation of its expression at RNA level under salt stress. Deduced amino acid sequence of AmUBC2 showed 96% identity with UBC2 of Arabidopsis thaliana and also 73-78% identity with RAD6 DNA repair protein of Homo sapiens, Rattus norvegicus, Caenorhabditis elegans, Drosophila melanogaster, Arabidopsis thaliana and Saccharomyces cerevisiae. Multiple alignment analysis showed that the amino acid residues in the core region of UBC2 were highly conserved across different taxa in the evolutionary hierarchy. While some ubiquitin conjugating enzymes were induced under salt, heat and heavy metal stress in different tissues in plants, Northern analysis in the present study has clearly shown that the expression of UBC2 is not induced by salt stress either in root or in leaf tissues in A. marina. Southern hybridization of genomic DNA with gene-specific probe showed that AmUBC2 is a single copy gene

Species identification in seven small millet species using polymerase chain reaction - restriction fragment length polymorphism of trnS-psbC gene region

The chloroplast trnS-psbC gene regions from total genomic DNA of 119 accessions from seven small millet species were amplified by polymerase chain reaction (PCR) and digested with eight restriction enzymes individually as well as in combinations of two enzymes to generate restriction fragment length polymorphism (PCR-RFLP). PCR-RFLP with individual enzymes revealed polymorphism between only some species. However, all the species could be distinguished by using a combination of two enzymes, specifically HaeIII and MspI. PCR-RFLP of 11 to 20 accessions with the same enzyme combination showed no intraspecific variation, which established that the differential banding patterns were species specific. In contrast, the same enzyme combination was not useful for differentiating different species of the genera Cajanus, Rhyncosia, Abies, Rhizophora, Ceriops, and Bruguiera, and it also revealed intraspecies variation in three species of Abies. The present study indicated that digestion of trnS-psbC with two four-base recognizing enzymes reveals more variation than with either enzyme alone and that it may be a method of choice for species identification in some genera