The Subsurface Geology and Landscape Evolution of the Volturno Coastal Plain, Italy: Interplay between Tectonics and Sea-Level Changes during the Quaternary

The Volturno alluvial-coastal plain is a relevant feature of the Tyrrhenian side of Southern Italy. Its plan-view squared shape is due to Pliocene-Quaternary block-faulting of the western flank of the south-Apennines chain. On the basis of the stratigraphic analysis of almost 700 borehole logs and new geomorphological survey, an accurate paleoenvironmental reconstruction before and after the Campania Ignimbrite (CI; about 40 ky) eruption is here presented. Tectonics and eustatic forcing have been both taken into account to completely picture the evolution of the coastal plain during Late Quaternary times. The upper Pleistocene-Holocene infill of the Volturno plain has been here re-organized in a new stratigraphic framework, which includes seven depositional units. Structural analysis showed that two sets of faults displaced the CI, so accounting for recent tectonic activity. Yet Late Quaternary tectonics is rather mild, as evidenced by the decametric vertical separations operated by those faults. The average slip rate, which would represent the tectonic subsidence rate of the plain, is about 0.5 mm/year. A grid of cross sections shows the stratigraphic architecture which resulted from interactions among eustatic changes, tectonics and sedimentary input variations. On the basis of boreholes analysis, the trend of the CI roof was reconstructed. An asymmetrical shape of its ancient morphology - with a steeper slope toward the north-west border - and the lack of coincidence between the present course of the Volturno River and the main buried bedrock incision, are significant achievements of this study. Finally, the morpho-evolutionary path of the Volturno plain has been discussed

Regenerating the human heart : direct reprogramming strategies and their current limitations

Cardiovascular diseases are the leading cause of death in the Western world. Unfortunately, current therapies are often only palliative, consequently essentially making heart transplantation necessary for many patients. However, several novel therapeutic approaches in the past two decades have yielded quite encouraging results. The generation of induced pluripotent stem cells, through the forced expression of stem cell-specific transcription factors, has inspired the most promising strategies for heart regeneration by direct reprogramming of cardiac fibroblasts into functional cardiomyocytes. Initial attempts at this reprogramming were conducted using a similar approach to the one used with transcription factors, but during years, novel strategies have been tested, e.g., miRNAs, recombinant proteins and chemical molecules. Although preliminary results on animal models are promising, the low reprogramming efficiency, as well as the incomplete maturation of the cardiomyocytes, still represents important obstacles. This review covers direct transdifferentiation strategies that have been proposed and developed and illustrates the pros and cons of each approach. Indeed, as described in the manuscript, there are still many unanswered questions and drawbacks that require a better understanding of the basic signaling pathways and transcription factor networks before functional cells, suitable for cardiac regeneration and safe for the patients, can be generated and used for human therapies

Stratigrafia ed assetto geometrico dell’Unità del Sannio nel settore settentrionale dei monti del Matese

New stratigraphic and biostratigraphic data arising from the realization of the Sheet No. 405 "Campobasso" of the new Geological map of Italy (1:50.000 scale - CARG Project) allowed, for the first time in this area, to stratigraphically and cartographically define all the ranges composing the basinal Sannio Unit Auct.. Structural analysis and the chronostratigraphic redefinition of siliciclastic deposits covering the Sannio Unit and the carbonate platform successions of the Matese- Frosolone Units, indicate two main evolutionary stages in the Miocene- Pliocene structuring of this portion of the Southern Apennines. In the first stage, starting before Serravallian times, E-verging contraction affected exclusively the basinal units together with their siliciclastic cover. During the second stage, beginning after early Messinian times, NE-verging compression involved both the basinal Sannio Unit and the Matese-Frosolone Units.UnpublishedISPRA - Roma, Italy2.2. Laboratorio di paleomagnetismorestricte

Genotype-Phenotype Correlation in a Family with Brugada Syndrome Harboring the Novel p.Gln371* Nonsense Variant in the SCN5A Gene

Brugada syndrome (BrS) is marked by coved ST-segment elevation and increased risk of sudden cardiac death. The genetics of this syndrome are elusive in over half of the cases. Variants in the SCN5A gene are the single most common known genetic unifier, accounting for about a third of cases. Research models, such as animal models and cell lines, are limited. In the present study, we report the novel NM_198056.2:c.1111C>T (p.Gln371*) heterozygous variant in the SCN5A gene, as well as its segregation with BrS in a large family. The results herein suggest a pathogenic effect of this variant. Functional studies are certainly warranted to characterize the molecular effects of this variant

Low incidence of permanent complications during catheter ablation for atrial fibrillation using open-irrigated catheters: A multicentre registry

Aims Despite catheter ablation (CA) has become an accepted treatment option for symptomatic, drug-resistant atrial fibrillation (AF), the safety of this procedure continues to be cause for concern. The aim of the present study was to assess the incidence of complications with permanent sequelae of CA for AF using open-irrigated catheters in a contemporary, unselected population of consecutive patients. Methods and results From 1 January 2011 to 31 December 2011, data from 2167 consecutive patients who underwent CA for AF using an open-irrigated catheter in 29 Italian centres were collected. All the complications occurring to the patient from admission to the 30th post-procedural day were recorded. No procedure-related death was observed. Complications occurred in 81 patients (3.7%): 46 patients (2.1%) suffered vascular access complications; 13 patients (0.6%) cardiac tamponade, successfully drained in all the cases; six patients (0.3%) arterial thromboembolism (four transient ischaemic attack and two ischaemic strokes); five (0.2%) patients conservatively treated pericardial effusion; three patients (0.1%) phrenic nerve paralysis; three patients (0.1%) pericarditis; three patients (0.1%) haemothorax, and two patients (0.1%) other isolated adverse events. At multivariate analysis, only female sex [odds ratio (OR) 2.5, confidence interval (CI): 1.5-3.7, P < 001] and the operator experience (OR 0.5, CI: 0.4-0.7, P < 001) related to the complications. Only five (0.2%) patients developed permanent sequelae from their complications. Conclusion Catheter ablation for AF with the use of open-irrigated catheters is currently affected by a very low rate of complications leading to permanent sequelae. \uc2\ua9 2014 Published on behalf of the European Society of Cardiology

Influence of Age and Gender on Complications of Catheter Ablation for Atrial Fibrillation

Background: Despite catheter ablation (CA) has become an accepted treatment option for symptomatic, drug-resistant atrial fibrillation (AF), safety of this procedure continues to be cause for concern. Objective: Aim of the present multicenter study was to assess the influence of age and gender on incidence and severity of early CA complications. Methods: From January 1, 2011 to December 31, 2011, data from 2,323 consecutive patients who underwent CA (mean age 59.1+10.9; 72.3% male) for AF in 29 Italian centres were collected. All complications occurring to the patients from admission to 30th post-procedural day were recorded. Results: Complications occurred in 94 patients (4.0%); of these 7 (0.30%) developed permanent sequelae. There was a significant trend toward a greater incidence of complications with increasing age-group. In particular, the incidence of complications was 35/1066 (3.3%) in patients 60 year-old (p=0.03). All 7 patients with permanent sequeale were older than 60. Females had a higher incidence of complications both among younger [13/231 (5.6%) vs 22/915 (2.5%), p=0.02] and older patients [32/405 (7.9%) vs 27/739 (3.5%) p=0.001]. In subjects older than 60, 5/405 (1.2%) females and 2/176 (0.3%) males (p=0.04) suffered from permanent sequelae. Conclusions: Older patients and females are a subgroup at higher risk of complications during AF ablation. A particular care should be taken when performing CA in this clinical setting

GM1 Ganglioside Promotes Osteogenic Differentiation of Human Tendon Stem Cells

Gangliosides, the sialic acid-conjugated glycosphingolipids present in the lipid rafts, have been recognized as important regulators of cell proliferation, migration, and apoptosis. Due to their peculiar localization in the cell membrane, they modulate the activity of several key cell receptors, and increasing evidence supports their involvement also in stem cell differentiation. In this context, herein we report the role played by the ganglioside GM1 in the osteogenic differentiation of human tendon stem cells ( hTSCs). In particular, we found an increase of GM1 levels during osteogenesis that is instrumental for driving the process. In fact, supplementation of the ganglioside in the medium significantly increased the osteogenic differentiation capability of hTSCs. Mechanistically, we found that GM1 supplementation caused a reduction in the phosphorylation of the platelet-derived growth factor receptor-ss ( PDGFR-ss), which is a known inhibitor of osteogenic commitment. These results were further corroborated by the observation that GM1 supplementation was able to revert the inhibitory effects on osteogenesis when the process was inhibited with exogenous PDGF