CLINICO-DIAGNOSТIC STUDIES OF SELLEK-FRADE'S ТЕSТ IN PAТIENTS WIТH EPIDEMIC HEPATIТIS

In recent years, due to its mass spread, epidemic hepatitis became а medical рroblem of world-wide importance. Оnе of the still unsatisfactorily solved prblems in connection with this infectious disease is the question related to its diagnosis. Extensive efforts are made to introduce appropriate and reliable clinico-laboratory test particularly having in mind the lack of trustworthy аnd practical applicble specific microbiological tests for etiological diagnosis.Informed from the literature about а new test - that of Sellek-Frade, we set ourselves the task to prove the diagnootic value of this test in оur patients, suffering from epidemic hepatitis.The test is introduced bу Sellek and Fгade in 1957 in Наbапа. The author recommend this test as а very easily performed flocculation test with а considerble diagпostic importance in hepatic disorders. The test is examined later in the clinic of А. Lemaire in Paris.Тhe test of Sellek and Frade is а colloid-stability test, in which cuprum acetate being used as а substance, which disturbs the colloid stability of blооd serum. Similar to most other colloid-stability tests this best becomes positive when the relation between albumins and globulins is disturbed

Solvation dynamics in a Brownian dipole lattice: a comparison between theory and computer simulation

Papazyan and Maroncelli [J. Chem. Phys. 95, 9219 (1991)] recently reported computer simulations of solvation dynamics of an ion in a Brownian dipole lattice solvent. In the present article we compare these results to predictions of a number of theories of solvation dynamics in the diffusive limit. The frequency-dependent dielectric response functions needed as input to many of the theories are derived from further simulations of the lattice solvent [H. X. Zhou and B. Bagchi, J. Chem. Phys. 97, 3610 (1992)]. When properly applied, all of the currently popular molecular theories yield reasonable predictions for the time scale of the solvation response. The dynamical MSA model [P. G. Wolynes, J. Chem. Phys. 86, 5133 (1987)] and the memory function theory of Fried and Mukamel [J. Chem. Phys. 93, 932 (1990)] both provide nearly quantitative agreement with all aspects of the solvation dynamics observed in these simulations

Перспективы производства и использования сока ревеня

The review is devoted to the nutritional significance and prospects of garden rhubarb (Rheum rhabarbarum L.) utilization in food industry. High yield of juice reaching 90% and the ability to complex rhubarb stems processing for juice production and pectin extraction from rhubarb stem pomace are empathized. Rhubarb stems pomace recorded up to 21-23 % of pectin, which is significantly higher than in natural industrial sources of pectin. Medicinal value of rhubarb juice is discussed: antioxidant, anti-inflammatory, anti-carcinogenic, cardioprotective and anti-diabetic properties are indicated. Examples of high antioxidant content and unique organic acids composition of rhubarb juice are highlighted. Sorbic and benzoic acids are indicated as important components of juice widely used in food industry as food preservatives. Citric acid is shown to be the main component of rhubarb organic acids in spring. Special attention is paid to the non-waste production of juice thanks to the possibility of pomace processing for pectin recovery. Juice biochemical characteristics of four garden rhubarb cultivars (selection of Federal Scientific Center of Vegetable Production) are described: Udalets, Malakhit, Zaryanka and Krupnochereshkovy). Expediency of further selection on high anthocyanin content in rhubarb stems are empathized.Обзор посвящен пищевой ценности и перспективам использования сока ревеня садового (Rheum rhabarbarum L.) в пищевой промышленности. Отмечается высокий выход сока, достигающий 90%, а также возможность комплексной переработки сырья для получения сока и пектина из жмыха. Показано, что жмых черешков ревеня содержит от 21 до 23% пектина, что достоверно выше, чем в используемых в настоящее время природных источниках пектина. Обсуждается лекарственная ценность продукта, проявляющего антиоксидантное, противовоспалительное, противораковое, кардиопротекторное и антидиабетическое действие. Приводятся примеры высокого содержания антиоксидантов в соке и уникальный компонентный состав органических кислот, включая сорбиновую и бензойную, применяемых в качестве стабилизаторов в продуктах питания. Отмечается, что основной органической кислотой ревеня садового на ранней стадии развития (весной) является лимонная кислота. Особое внимание уделяется безотходности производства сока из черешков ревеня благодаря перспективам применения жмыха как значимого источника пектина. Приведена биохимическая характеристика сока 4-х сортов ревеня садового селекции ФГБНУ ФНЦО: Удалец, Малахит, Зарянка и Крупночерешковый. Отмечается целесообразность осуществления селекции ревеня на повышенное содержание антоцианов

Free Energy Simulations of a GTPase: GTP and GDP Binding to Archaeal Initiation Factor 2

International audienceArchaeal initiation factor 2 (aIF2) is a protein involved in the initiation of protein biosynthesis. In its GTP-bound, "ON" conformation, aIF2 binds an initiator tRNA and carries it to the ribosome. In its GDP-bound, "OFF" conformation, it dissociates from tRNA. To understand the specific binding of GTP and GDP and its dependence on the ON or OFF conformational state of aIF2, molecular dynamics free energy simulations (MDFE) are a tool of choice. However, the validity of the computed free energies depends on the simulation model, including the force field and the boundary conditions, and on the extent of conformational sampling in the simulations. aIF2 and other GTPases present specific difficulties; in particular, the nucleotide ligand coordinates a divalent Mg(2+) ion, which can polarize the electronic distribution of its environment. Thus, a force field with an explicit treatment of electronic polarizability could be necessary, rather than a simpler, fixed charge force field. Here, we begin by comparing a fixed charge force field to quantum chemical calculations and experiment for Mg(2+):phosphate binding in solution, with the force field giving large errors. Next, we consider GTP and GDP bound to aIF2 and we compare two fixed charge force fields to the recent, polarizable, AMOEBA force field, extended here in a simple, approximate manner to include GTP. We focus on a quantity that approximates the free energy to change GTP into GDP. Despite the errors seen for Mg(2+):phosphate binding in solution, we observe a substantial cancellation of errors when we compare the free energy change in the protein to that in solution, or when we compare the protein ON and OFF states. Finally, we have used the fixed charge force field to perform MDFE simulations and alchemically transform GTP into GDP in the protein and in solution. With a total of about 200 ns of molecular dynamics, we obtain good convergence and a reasonable statistical uncertainty, comparable to the force field uncertainty, and somewhat lower than the predicted GTP/GDP binding free energy differences. The sign and magnitudes of the differences can thus be interpreted at a semiquantitative level, and are found to be consistent with the experimental binding preferences of ON- and OFF-aIF2

Sochi State University as a Driver of Event Volunteering in the Region

The development of the volunteer movement is one of the important tasks of the state policy of modern Russia. Today, many universities create on their basis volunteer centers (EC), which are one of the best mechanisms for attracting young people to solving urgent problems of the development of society, an additional pedagogical and personal-developing resource. This article presents the results of the implementation by Sochi State University (SSU), which is a pillar higher education institution of the Krasnodar Territory, the strategic project “Resource Center for Event Volunteering”, aimed at ensuring the sustainable development of volunteering in the region and the Russian Federation as a whole. Volunteering is viewed as an institution of civil society and the basis for implementing long-term socio-cultural projects. The article describes the experience of the SSU in the development of volunteerism in the region, the improvement of its resource provision, the establishment of cooperation in solving these problems with the authorities, administration and the management of volunteer organizations of pillar universities and the region. A flexible universal model of training volunteers and organizers of the volunteer movement, organizational and managerial conditions and scientific and methodological approaches to the organization of student volunteering are presented. The results of their implementation in the region are described. The authors substantiate the expediency of the creation of volunteering resource centers in the regions, providing educational organizations with a range of information, advisory and methodological services in the field of volunteering

Effect of organic selenium on goose reproduction

For the last few years a great body of scientific information indicates that organic selenium is more effective in transferring to the egg and the developing embryo in comparison to sodium selenite. The aim of the study was to evaluate effects of the replacement of sodium selenite by organic selenium in the form of Sel-Plex (Altech, USA) in goose diets in commercial conditios. In the experiment 5672 Italian White Goose Breeders were divided into 3 groups. Control group was fed on the commercial diet without Se supplementation, second group was supplemented by sodium selenite (0.3 ppm Se) and third group was supplemented with the same amopunt of Se (0.3 ppm) in the form of Sel-Plex during the laying period. Fertility was shown to be 93.4, 96.7 and 97.1%, while hatchability of fertile eggs was 67.2, 71.3 and 75% in the control and experimental groups respectively. The weight of the day-old gooslings was shown to be 93.9, 96.4 and 98.8 g respectively. Therefore, these data indicate that the replacement of the sodium selenite by Sel-Plex could improve reproductive performance of geese in commercial conditions.vokMyynti MTT Tietopalvelut 31600 Jokioine