Performance evaluation of flooding in MANETs in the presence of multi-broadcast traffic

Broadcasting has many important uses and several mobile ad hoc networks (MANETs) protocols assume the availability of an underlying broadcast service. Applications, which make use of broadcasting, include LAN emulation, paging a particular node. However, broadcasting induces what is known as the "broadcast storm problem" which causes severe degradation in network performance, due to excessive redundant retransmission, collision, and contention. Although probabilistic flooding has been one of the earliest suggested approaches to broadcasting. There has not been so far any attempt to analyse its performance behaviour in MANETs. This paper investigates using extensive ns-2 simulations the effects of a number of important parameters in a MANET, including node speed, pause time and, traffic load, on the performance of probabilistic flooding. The results reveal that while these parameters have a critical impact on the reachability achieved by probabilistic flooding, they have relatively a lower effect on the number of saved rebroadcast packets

Performance evaluation of adjusted probabilistic broadcasting in MANETs

Appropriate use of a probabilistic broadcasting method in MANETs can decrease the number of rebroadcasts, and as a result reduce the opportunity of contention and collision among neighbouring nodes. In this paper we evaluate the performance of adjusted probabilistic flooding by comparing it to "simple" flooding as used with the ad hoc on demand distance vector (AODV) routing protocol as well as a fixed probabilistic approach. The results reveal that the adjusted probabilistic flooding exhibits superior performance in terms of both reachability and saved rebroadcast

Improving route discovery in on-demand routing protocols using local topology information in MANETs

Most existing routing protocols proposed for MANETs use flooding as a broadcast technique for the propagation of network control packets; a particular example of this is the dissemination of route requests (RREQs), which facilitate route discovery. In flooding, each mobile node rebroadcasts received packets, which, in this manner, are propagated network-wide with considerable overhead. This paper improves on the performance of existing routing protocols by reducing the communication overhead incurred during the route discovery process by implementing a new broadcast algorithm called the adjusted probabilistic flooding on the Ad-Hoc on Demand Distance Vector (AODV) protocol. AODV [3] is a well-known and widely studied algorithm which has been shown over the past few years to maintain an overall lower routing overhead compared to traditional proactive schemes, even though it uses flooding to propagate RREQs. Our results, as presented in this paper, reveal that equipping AODV with fixed and adjusted probabilistic flooding, instead, helps reduce the overhead of the route discovery process whilst maintaining comparable performance levels in terms of saved rebroadcasts and reachability as achieved by conventional AODV\@. Moreover, the results indicate that the adjusted probabilistic technique results in better performance compared to the fixed one for both of these metrics

On the performance of probabilistic flooding in mobile ad hoc networks

This paper investigates using extensive simulations the effects of a number of important system parameters in a typical MANETs, including node speed, pause time, traffic load, and node density on the performance of probabilistic flooding. The results reveal that most of these parameters have a critical impact on the reachability and the number of saved rebroadcast messages achieved by probabilistic flooding, prompting the need for dynamically adjusting nodal retransmission probabilities depending on the current state of the network

Impact of thixotropy on flow patterns induced in a stirred tank : numerical and experimental studies

Agitation of a thixotropic shear-thinning fluid exhibiting a yield stress is investigated both experimentally and via simulations. Steady-state experiments are conducted at three impeller rotation rates (1, 2 and 8 s−1) for a tank stirred with an axial-impeller and flow-field measurements are made using particle image velocimetry (PIV) measurements. Threedimensional numerical simulations are also performed using the commercial CFD code ANSYS CFX10.0. The viscosity of the suspension is determined experimentally and is modelled using two shear-dependant laws, one of which takes into account the flow instabilities of such fluids at low shear rates. At the highest impeller speed, the flow exhibits the familiar outward pumping action associated with axial-flow impellers. However, as the impeller speed decreases, a cavern is formed around the impeller, the flow generated in the vicinity of the agitator reorganizes and its pumping capacity vanishes. An unusual flow pattern, where the radial velocity dominates, is observed experimentally at the lowest stirring speed. It is found to result from wall slip effects. Using blades with rough surfaces prevents this peculiar behaviour and mainly resolves the discrepancies between the experimental and computational results

Stringent and reproducible tetracycline-regulated transgene expression by site-specific insertion at chromosomal loci with pre-characterised induction characteristics

<p>Abstract</p> <p>Background</p> <p>The ability to regulate transgene expression has many applications, mostly concerning the analysis of gene function. Desirable induction characteristics, such as low un-induced expression, high induced expression and limited cellular heterogeneity, can be seriously impaired by chromosomal position effects at the site of transgene integration. Many clones may therefore need to be screened before one with optimal induction characteristics is identified. Furthermore, such screens must be repeated for each new transgene investigated, and comparisons between clones with different transgenes is complicated by their different integration sites.</p> <p>Results</p> <p>To circumvent these problems we have developed a "screen and insert" strategy in which clones carrying a transgene for a fluorescent reporter are first screened for those with optimal induction characteristics. Site-specific recombination (SSR) is then be used repeatedly to insert any new transgene at the reporter transgene locus of such clones so that optimal induction characteristics are conferred upon it. Here we have tested in a human fibrosarcoma cell line (HT1080) two of many possible implementations of this approach. Clones (e.g. Rht14-10) in which a GFP reporter gene is very stringently regulated by the tetracycline (tet) transactivator (tTA) protein were first identified flow-cytometrically. Transgenes encoding luciferase, I-<it>Sce</it>I endonuclease or Rad52 were then inserted by SSR at a <it>LoxP </it>site adjacent to the GFP gene resulting stringent tet-regulated transgene expression. In clone Rht14-10, increases in expression from essentially background levels (+tet) to more than 10⁴-fold above background (-tet) were reproducibly detected after Cre-mediated insertion of either the luciferase or the I-<it>Sce</it>I transgenes.</p> <p>Conclusion</p> <p>Although previous methods have made use of SSR to integrate transgenes at defined sites, none has effectively combined this with a pre-selection step to identify integration sites that support optimal regulatory characteristics. Rht14-10 and similar HT1080-derived clones can now be used in conjunction with a convenient delivery vector (pIN2-neoMCS), in a simple 3-step protocol leading to stringent and reproducible transgene regulation. This approach will be particularly useful for transgenes whose products are very active at low concentrations and/or for comparisons of multiple related transgenes.</p

Synthesis and Evaluation of Nifurtimox-Adamantane Adducts with Trypanocidal Activity.

The synthesis and pharmacological evaluation of C1-substituted adamantane hydrazones, their C2-substituted isomers, and C1-substituted adamantane furanoic carboxamides is described. These new adamantane derivatives exhibited an interesting pharmacological profile in terms of trypanocidal activity and selectivity. The most active adduct with the best selectivity in this study was found to be the phenylacetoxy hydrazone 1 b (2-[4-(tricyclo[3.3.1.13,7 ]dec-1-yl)phenyl]-N'-[(5-nitrofuran-2-yl)methylene]acetohydrazide; EC50 =11±0.9 nm, SITb =770)