Different types of events presented by the CellSearch System.

<p>1–2. Two classical examples of CTC fulfilling all the Veridex criteria: A) intact round to oval cells positive for epithelial cell marker (CK-PE) of more than 4 µm in size; B) positivity for the nuclear dye (DAPI) in an area smaller than the cytoplasmic area inside the cytoplasm (at least 50%); C) Negativity for the leucocyte marker (CD45/APC); D) negativity in the blank channel. 3. A suspicious object satisfying only A, C, and D criteria. 4. A large naked nucleus. 5. A cluster of naked nuclei.</p

Comparison of <i>EGFR</i> mutations in primary tumors and CTC preparations.

<p>Footnotes: CTCs, Circulating Tumor Cells; SS, Sanger sequencing; NGS, next generation sequencing.</p><p>*case with double or multiple mutations.</p><p>**blood-derived material in the Cell Search cartridge containing CTCs or potential neoplastic elements.</p