The clock gene period in the medfly Ceratitis capitata.

We have isolated the clock gene period (per) from the medfly Ceratitis capitata, one of the most economically important insect pest species. The overall pattern of conserved, non-conserved and functional domains that are observed within dipteran and lepidopteran per orthologues is preserved within the coding sequence. Expression analysis from fly heads revealed a daily oscillation in per mRNA in both light[ratio ]dark cycles and in constant darkness. However PER protein levels from head extracts did not show any significant evidence for cycling in either of these two conditions. When the Ceratitis per transgene under the control of the Drosophila per promoter and 3′UTR was introduced into Drosophila per-null mutant hosts, the transformants revealed a low level of rescue of behavioural rhythmicity. Nevertheless, the behaviour of the rhythmic transformants showed some similarities to that of Ceratitis, suggesting that Ceratitis per carries species-specific information that can evidently affect the Drosophila host's downstream rhythmic behaviour

Description of Euplotes margherensis nov. spec.(Ciliophora, Hypotrichida) from the Lagoon ofVenice

A new Euplotes morphospecies, E. margherensis nov. spec, belonging to the group of species characterized by a dorsal silverline system of the \uabdouble\ubb type and a set of 10 frontoventral cirri, is described. It was isolated from a sample collected in a central area of the Lagoon of Venice. It is of small dimensions, about 50 7 40 \u3bcm, and is endowed with a small projection on the right ventral front and with an indented margin which bounds the right side of the adoral zone of membranelles

Scrivere testi in 9 mosse. Curricolo verticale di scrittura per la scuola primaria e secondaria di primo grado

Il volume propone un metodo innovativo per accompagnare docenti e alunni di scuola primaria e secondaria di primo grado nell\u2019insegnamento e apprendimento dei processi di scrittura, con proposte operative flessibili.Facendo riferimento agli studi di matrice cognitivista e socio-costruttivista, si forniscono indicazioni metodologiche e materiali per allestire contesti laboratoriali centrati sulle tipologie di testo narrativo, espositivo, argomentativo.Una serie di Guide procedurali, metacognitive e per l\u2019autoregolazione con schede, questionari e percorsi di automonitoraggio promuovono l\u2019autonomia degli alunni nell\u2019impiego di strategie per la produzione del testo scritto.All\u2019insegnante sono rivolti suggerimenti e strumenti per allestire ambienti funzionali all\u2019apprendimento dello scrivere, condurre percorsi laboratoriali per insegnare a comporre testi, valutare le produzioni scritte.All\u2019alunno sono destinate schede e attivit\ue0 per imparare a scrivere per raccontare, scrivere per studiare e per esporre, scrivere per sostenere idee e convincere.La sezione dei Repertori presenta ricche risorse per produrre testi in un contesto partecipativo ancora pi\uf9 coinvolgente e motivante

Leigh syndrome in Drosophila melanogaster: morphological and biochemical characterization of Surf1 post-transcriptional silencing.

Leigh Syndrome (LS) is the most common early-onset, progressive mitochondrial encephalopathy usually leading to early death. The single most prevalent cause of LS is occurrence of mutations in the SURF1 gene, and LS(Surf1) patients show a ubiquitous and specific decrease in the activity of mitochondrial respiratory chain complex IV (cytochrome c oxidase, COX). SURF1 encodes an inner membrane mitochondrial protein involved in COX assembly. We established a Drosophila melanogaster model of LS based on the post-transcriptional silencing of CG9943, the Drosophila homolog of SURF1. Knockdown of Surf1 was induced ubiquitously in larvae and adults, which led to lethality; in the mesodermal derivatives, which led to pupal lethality; or in the central nervous system, which allowed survival. A biochemical characterization was carried out in knockdown individuals, which revealed that larvae unexpectedly displayed defects in all complexes of the mitochondrial respiratory chain and in the F-ATP synthase, while adults had a COX-selective impairment. Silencing of Surf1 expression in Drosophila S2R(+) cells led to selective loss of COX activity associated with decreased oxygen consumption and respiratory reserve. We conclude that Surf1 is essential for COX activity and mitochondrial function in D. melanogaster, thus providing a new tool that may help clarify the pathogenic mechanisms of LS

Post-transcriptional Silencing and Functional Characterization of the Drosophila melanogaster Homolog of Human Surf1

Mutations in Surf1, a human gene involved in the assembly of cytochrome c oxidase (COX), cause Leigh syndrome, the most common infantile mitochondrial encephalopathy, characterized by a specific COX deficiency. We report the generation and characterization of functional knockdown (KD) lines for Surf1 in Drosophila. KD was produced by post-transcriptional silencing employing a transgene encoding a dsRNA fragment of the Drosophila homolog of human Surf1, activated by the UAS transcriptional activator. Two alternative drivers, Actin5C–GAL4 or elav–GAL4, were used to induce silencing ubiquitously or in the CNS, respectively. Actin5C–GAL4 KD produced 100% egg-to-adult lethality. Most individuals died as larvae, which were sluggish and small. The few larvae reaching the pupal stage died as early imagos. Electron microscopy of larval muscles showed severely altered mitochondria. elav–GAL4-driven KD individuals developed to adulthood, although cephalic sections revealed low COX-specific activity. Behavioral and electrophysiological abnormalities were detected, including reduced photoresponsiveness in KD larvae using either driver, reduced locomotor speed in Actin5C–GAL4 KD larvae, and impaired optomotor response as well as abnormal electroretinograms in elav–GAL4 KD flies. These results indicate important functions for SURF1 specifically related to COX activity and suggest a crucial role of mitochondrial energy pathways in organogenesis and CNS development and function