Maternal diet-induced obesity during pregnancy alters lipid supply to mouse E18.5 fetuses and changes the cardiac tissue lipidome in a sex- dependent manner

Maternal obesity during pregnancy has immediate and long-term detrimental effects on the offspring heart. In this study, we characterized the cardiac and circulatory lipid profiles in late gestation E18.5 fetuses of diet-induced obese pregnant mice and established the changes in lipid abundance and fetal cardiac transcriptomics. We used untargeted and targeted lipidomics and transcriptomics to define changes in the serum and cardiac lipid composition and fatty acid metabolism in male and female fetuses. From these analyses we observed: (1) maternal obesity affects the maternal and fetal serum lipidome distinctly; (2) female fetal heart lipidomes are more sensitive to maternal obesity than males; (3) changes in lipid supply might contribute to early expression of lipolytic genes in mouse hearts exposed to maternal obesity. These results highlight the existence of sexually dimorphic responses of the fetal heart to the same in utero obesogenic environment and identify lipids species that might mediate programming of cardiovascular health.MRC Metabolic Diseases Unit [MRC_MC_UU_00014/4] Cambridge Home and EU Student Scholarship British Heart Foundation studentship [FS/12/64/30001] II was supported by a British Heart Foundation studentship [FS/18/56/35177

Effects of maternal diet-induced obesity on metabolic disorders and age-associated miRNA expression in the liver of male mouse offspring.

OBJECTIVE: This study investigated the effect of maternal obesity on aged-male offspring liver phenotype and hepatic expression of a programmed miRNA. METHODS: A mouse model (C57BL/6 J) of maternal diet-induced obesity was used to investigate fasting-serum metabolites, hepatic lipid content, steatosis, and relative mRNA levels (RT-PCR) and protein expression (Western blotting) of key components involved in hepatic and mitochondrial metabolism in 12-month-old offspring. We also measured hepatic lipid peroxidation, mitochondrial content, fibrosis stage, and apoptosis in the offspring. To investigate potential mechanisms leading to the observed phenotype, we also measured the expression of miR-582 (a miRNA previously implicated in liver cirrhosis) in 8-week-old and 12-month-old offspring. RESULTS: Body weight and composition was similar between 8-week-old offspring, however, 12-month-old offspring from obese mothers had increased body weight and fat mass (19.5 ± 0.8 g versus 10.4 ± 0.9 g, p < 0.001), as well as elevated serum levels of LDL and leptin and hepatic lipid content (21.4 ± 2.1 g versus 12.9 ± 1.8 g, p < 0.01). This was accompanied by steatosis, increased Bax/Bcl-2 ratio, and overexpression of p-SAPK/JNK, Tgfβ1, Map3k14, and Col1a1 in the liver. Decreased levels of Bcl-2, p-AMPKα, total AMPKα and mitochondrial complexes were also observed. Maternal obesity was associated with increased hepatic miR-582-3p (p < 0.001) and miR-582-5p (p < 0.05). Age was also associated with an increase in both miR-582-3p and miR-582-5p, however, this was more pronounced in the offspring of obese dams, such that differences were greater in 12-month-old animals (-3p: 7.34 ± 1.35 versus 1.39 ± 0.50, p < 0.0001 and -5p: 4.66 ± 1.16 versus 1.63 ± 0.65, p < 0.05). CONCLUSION: Our findings demonstrate that maternal diet-induced obesity has detrimental effects on offspring body composition as well as hepatic phenotype that may be indicative of accelerated-ageing phenotype. These whole-body and cellular phenotypes were associated with age-dependent changes in expression of miRNA-582 that might contribute mechanistically to the development of metabolic disorders in the older progeny

Associação entre risco de disfagia e risco nutricional em idosos internados em hospital universitário de Brasília Association between risk of dysphagia and nutritional risk in elderly inpatients at a univesity hospital of Brasília, Brazil

OBJETIVOS: Investigar a associação entre o grau de risco de disfagia e o risco nutricional dos pacientes idosos internados na clínica médica do Hospital Universitário de Brasília. MÉTODOS: Estudo transversal, analítico, original, observacional, não-controlado, com amostra de conveniência e estratificada. Foram aplicados a Mini Avaliação Nutricional e o questionário de risco de disfagia em 49 pacientes idosos nas primeiras 48 horas após admissão na clínica médica, em setembro de 2006. RESULTADOS: A freqüência de idosos foi maior na faixa etária entre 60-74 anos 11meses (78%), com média de idade de 69 anos, sendo 41% mulheres e 59% homens. A proporção de risco de disfagia dos pacientes idosos foi de 69% e do estado nutricional inadequado de 71%, valor elevado principalmente entre as mulheres. Ambos os sexos se encontram em risco nutricional, masculino (20,5, Desvio-padrão - DP=5) e feminino (19, DP=6). O estudo mostrou que as mulheres apresentam uma tendência maior para risco de disfagia, porém os homens apresentam um quadro de maior gravidade (risco moderado). Foi encontrada uma correlação negativa moderada entre os valores de risco de disfagia e seus respectivos scores da Mini Avaliação Nutricional. CONCLUSÃO: Foi verificado neste estudo que os idosos apresentam dois agravantes de sua qualidade de vida: estado nutricional e disfagia, que podem ser agudizados na internação hospitalar.<br>OBJECTIVES: This study aimed to verify the association between the degree of risk of dysphagia and nutritional risk of elderly inpatients at the medical clinic of Brasília´s University Hospital. METHODS: This is a cross-sectional, analytic, original, observational, uncontrolled study with convenience and stratified sample. The Mini Nutritional Assessment and a questionnaire to assess risk of dysphagia were administered to 49 elderly patients within the first 48 hours after admission to the medical clinic, in September 2006. RESULTS: Most of the elderly inpatients aged from 60 to 74 years and 11 months (78%) with a mean age of 69 years; 41% were females and 59% were males. Sixty-nine percent of the elderly inpatients were at risk of dysphagia and 71% presented inadequate nutritional status, especially women. Both males (20.5, standard deviation= 5) and females (19, standard deviation= 6) were at nutritional risk. The study showed that women are more likely to develop dysphagia yet men presented a higher degree of risk (moderate risk). There is a moderate negative correlation between the values obtained for the risk of dysphagia and their respective Mini Nutritional Assessment scores. CONCLUSION: This study verified that two factors aggravate the quality of life of the elderly: their nutritional status and dysphagia. Both can worsen dramatically during hospitalization